Kiti yeKuona Acid yeDengue I/II/III/IV (Fluorescence PCR)-RUO ine Hwando Hwakaoma neFrize
Zita rechigadzirwa
Kiti yeKuona Acid yeDengue I/II/III/IV (Fluorescence PCR)-RUO ine Hwando Hwakaoma neFrize
Epidemiology
Dengue fever (DF), iyo inokonzerwa nehutachiona hwedenguevirus (DENV), ndeimwe yezvirwere zvinotapukira zve arbovirus zvakanyanya. Nzira yayo yekutapurirana inosanganisira Aedes aegypti neAedes albopictus. DF inonyanya kuwanikwa munzvimbo dzinopisa uye dziri pasi pemvura. DENV ndeye flavivirus iri pasi pe flaviviridae, uye inogona kuiswa muzvikamu zvina zvichienderana ne surface antigen. Zviratidzo zvechirwere cheDENV zvinosanganisira musoro, fivha, kushaya simba, kuwedzera kwe lymph node, leukopenia nezvimwewo, uye kubuda ropa, shock, kukuvara kwechiropa kana kunyange kufa muzviitiko zvakakomba. Mumakore achangopfuura, shanduko yemamiriro ekunze, kukura kwemaguta, kukura nekukurumidza kwevashanyi nezvimwe zvinhu zvakapa mamiriro ekukurumidza uye ari nyore ekutapurirana nekupararira kweDF, zvichikonzera kuwedzera nguva dzose kwenzvimbo yedenda yeDF. Nokudaro, kugadzwa kwenzira iri nyore, chaiyo uye inokurumidza yekuongorora chirwere cheDENV kune kukosha kwakakosha pakuongororwa kwechirwere cheDF.
Magadzirirwo ehunyanzvi
| Kuchengetera | 2-8℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | seramu |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Makopi 500/mL |
| Kunyatsojeka | Miedzo haina kunaka pakuongorora serum ye virus yedengue; mhinduro dzeongororo dzinoratidza kuti kana huwandu hwe bilirubin mu serum husingapfuure 168.2μmol/mL, huwandu hwe hemoglobin hunogadzirwa ne hemolysis husingapfuure 130g/L, huwandu hwe lipid muropa husingapfuure 65mmol/mL, uye huwandu hwese hwe IgG mu serum husingapfuure 5mg/mL, hapana chinokanganisa pakuona virus yedengue; shandisa virus yeHepatitis A, virus yeHepatitis B, virus yeHepatitis C, virus yeHerpes, virus yeEastern equine encephalitis, virus yeHantaan, virus yeBunia, virus yeWest Nile, virus yeZika uye sampuli dze serum ye genomic yevanhu kuti uongorore cross-reactivity, uye mhedzisiro yacho inoratidza kuti hapana cross reaction pakati pe kit iyi nehutachiona huri pamusoro apa. |
| Zvishandiso Zvinoshanda | Rudzi I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Rudzi rwechipiri: EudemonTMAIO800 (HWTS-EQ007) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Kufamba Kwebasa
Ma reagents anoda asi haana kupihwa:
Rudzi I: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Rudzi rwechipiri: Macro & Micro-Test General DNA/RNA Kit (HWTS-3023-8), inogona kushandiswa neEudemonTM AIO800 (HWTS-EQ007) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Usati washandisa Type II test reagent, mutengi ane reagent inotsigira yekuburitsa uye amplification, uye mainjiniya achatungamira mutengi kuti aite oparesheni yekuedza paanenge achishandisa kekutanga.
Zvinoshandiswa zvinodiwa asi hazvipihwe:Mazano eDNase/RNase Asina Kushandiswa, magirovhosi anoraswa, machubhu eEP Asina Kushandiswa eDNase/RNase, centrifuge, magnetic stand, 8-tube strips dzePCR.
