KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
Zita rechigadzirwa
KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
Epidemiology
Kuchinja kwemapoinzi mujini reKRAS kwakawanikwa mumhando dzakasiyana dzegomarara revanhu, huwandu hwe17% ~ 25% mukuchinja kwegomarara, huwandu hwe15% ~ 30% mukuchinja kwegomarara remapapu, huwandu hwe20% ~ 50% mukuchinja kwegomarara remudumbu. Nekuti puroteni yeP21 inonyorwa nejini reK-ras iri pasi penzira yechiratidzo yeEGFR, mushure mekuchinja kwejini reK-ras, nzira yechiratidzo yekudzika inogara ichishandiswa uye haikanganisirwi nemishonga inotarirwa kumusoro paEGFR, zvichikonzera kuwanda kwemasero ane kenza. Kuchinja kwejini reK-ras kunowanzo kupa kuramba kweEGFR tyrosine kinase inhibitors muvarwere vekenza yemapapu uye kuramba mishonga inorwisa anti-EGFR antibody muvarwere vekenza yemudumbu [1, 2, 3]. Muna 2008, National Comprehensive Cancer Network (NCCN) yakaburitsa gwara rekurapa kenza yemudumbu, iro rakaratidza kuti nzvimbo dzinokonzera kuchinja kwemasero dzinoita kuti K-ras ishande dziri mu codons 12 ne13 ye exon 2, uye rakakurudzira kuti varwere vese vane kenza yemudumbu yemudumbu inogona kuongororwa K-ras mutation vasati varapwa[4]. Nokudaro, kuona nekukurumidza uye kwakarurama K-ras gene mutation kwakakosha zvikuru mukurapa kwekiriniki. Kit iyi inoshandisa DNA semuyero wekuona kuti ipe kuongororwa kwemamiriro ekuchinja kwemasero, izvo zvinogona kubatsira vanachiremba mukuongorora kenza yemudumbu, kenza yemapapu nevamwe varwere vane bundu vanobatsirwa nemishonga inotarirwa. Mhedzisiro yekuedzwa kwekit iyi ndeyekutarisa kukiriniki chete uye haifanirwe kushandiswa senzira chete yekurapa varwere. Vanachiremba vanofanirwa kuita sarudzo dzakakwana pamhedzisiro yebvunzo zvichibva pazvinhu zvakaita semamiriro emurwere, zviratidzo zvemushonga, mhinduro yekurapa uye zvimwe zviratidzo zvebvunzo yerabhoritari.
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | zvikamu zvezvirwere zveparafini zvakaiswa muvanhu |
| CV | ≤5.0% |
| LoD | a) K-ras Reaction Buffer A uye K-ras Reaction Buffer B inogona kuona 1% mutation rate pasi pe3ng/μL wild-type background; b) Mutation ye1×103Makopi/mL anogona kuonekwa zvakanaka muchimiro chemusango che1 × 105Makopi/mL kana chiyero chekuchinja chiri 1%; c) Kana kambani LoD reference SW3 ikaedzwa, hapana kukosha kweCt kana kukosha kweCt=0 yeReaction Buffer A uye Reaction Buffer B |
| Zvishandiso Zvinoshanda | Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7300 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, LightCycler® 480 Real-Time PCR system, BioRad CFX96 Real-Time PCR System. |
Kufamba Kwebasa
Reagent inodiwa asi haina kupihwa:DNase/RNase isina H2O, ethanol isina mvura. Pakuyedza sampuro yenyama yakaputirwa neparafini, zvinokurudzirwa kushandisa QIAGEN's QIAamp DNA FFPE Tissue Kit (56404) uye Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yakagadzirwa neTiangen Biotech(Beijing) Co., Ltd.
Zvinodiwa asi hazvipihwe:Matipi asina DNase/RNase, magirovhosi anoraswa, chubhu yeCentrifuge isina DNase/RNase, zvidimbu zvemachubhu masere, centrifuge.
