Kiti yeKuona Kuchinja kweGene 29 yeHuman EGFR (Fluorescence PCR)-RUO

Kenza yemapapu yave chinhu chikuru chinokonzera kufa kwekenza pasi rose, zvichikonzera njodzi huru kuhutano hwevanhu. Kenza yemapapu isiri diki inoumba vanhu vanosvika 80%-85% yevarwere vane kenza yemapapu. EGFR ndiyo inonyanya kukosha pakurapa kenza yemapapu isiri diki. Kuumbwa kwe phosphorylation yeEGFR kunogona kukurudzira kukura kwemasero ekenza, kusiyanisa, kupinda mumasero, kupararira kwemasero, kurwisa apoptosis, uye kukurudzira angiogenesis yekenza. EGFR tyrosine kinase inhibitors (TKI) inogona kuvharira nzira yechiratidzo cheEGFR nekudzivisa EGFR autophosphorylation, nokudaro ichidzivisa kuwanda nekusiyana kwemasero ekenza, kukurudzira apoptosis yemasero ekenza, kuderedza angiogenesis yekenza, nezvimwewo, kuitira kuti iwane kurapwa kwakatariswa nekenza [1]. Ongororo dzakawanda dzakaratidza kuti kushanda kweEGFR-TKI pakurapa kwakabatana zvikuru nemamiriro ekuchinja kwemajini eEGFR, uye kunogona kudzivirira kukura kwemasero ekenza nekuchinja kwemajini eEGFR [2,3]. Jini reEGFR riri paruoko rupfupi rwechromosome 7 (7p12), rune hurefu hwakazara hwe200Kb uye rine maexons 28. Nzvimbo yekuchinja inowanikwa zvakanyanya mu exons 18 kusvika 21, codons 746 kusvika 753 deletion mutation pa exon 19 inosvika 45% uye L858R mutation pa exon 21 inosvika 40% kusvika 45% [4]. NCCN Guidelines for the Diagnosis and Treatment of Non-Small Cell Lung Cancer inotaura zvakajeka kuti bvunzo yekuchinja kwemajini eEGFR inodiwa EGFR-TKI isati yapihwa. Iyi test kit inoshandiswa kutungamira kupihwa kwemishonga ye epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), uye inopa hwaro hwemishonga yakagadzirirwa varwere vane kenza yemapapu isiri diki. Iyi kit inoshandiswa chete pakuona shanduko dzakajairika mu gene reEGFR muvarwere vane kenza yemapapu isiri diki. Mhedzisiro yebvunzo ndeyekutarisa kukiriniki chete uye haifanirwe kushandiswa senzira chete yekurapa varwere vakasiyana. Vanachiremba vanofanirwa kufunga nezvemamiriro emurwere, zviratidzo zvemushonga, uye kurapwa. Maitiro ake nezvimwe zviratidzo zvebvunzo yerabhoritari nezvimwe zvinhu zvinoshandiswa kutonga zvakakwana mhedzisiro yebvunzo.