Kiti yeKuona Nucleic Acid yeBundybugyo Virus Ebola (Fluorescence PCR)

Kiti yeKuona Acid yeBundybugyo Ebola Virus (Fluorescence PCR) Mufananidzo Unoratidzwa

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[Zita reChigadzirwa] Kiti yeKuona Nucleic Acid yeBundybugyo Virus Ebola (Fluorescence PCR)

[ChigadzirwaKodhi] HWTS-FE4023

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Chigadzirwa ichi chakagadzirirwa kuona nucleic acid kubva mu in vitro qualitative

Virusi reBundybugyo Ebola riri muropa rose revanhu, serum/plasma, uye sampuli dzemushonga wemuromo.

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Kiti yeKuona Nucleic Acid yeBundybugyo Virus Ebola (Fluorescence PCR)

Epidemiology

Filoviridae. Ndiyo hutachiona hwechitatu hweEbola hunogona kukonzera denda guru revanhu,zvichitevera Zaire ebolavirus (EBOV) neSudan ebolavirus (SUDV). BDBV chirwere chimwe chetehutachiona hweRNA hune tambo isina kunaka ine genome inosvika 19 kb, inokwana 7zvekuvakamapuroteni nemapuroteni maviri asina chimiro. Nguva yekuzvara kwehutachiona hweBDBV i2–21mazuva (avhareji mazuva 6.3). Zviratidzo zvayo zvakafanana zvikuru nezvemamwe mavhairasi eEbolauye hazvigone kusiyaniswa nezviratidzo zvekiriniki chete. Muna Nyamavhuvhu 2007,kubuda kwechirwere che "virusi hemorrhagic fever" chisina kutsanangurwa kwakaitika muBundibugyo District ofkumadokero kweUganda. Yakazivikanwa sechirwere chitsva cheEbola uye yakatumidzwa zita rekuti Bundibugyoebolavirus (BDBV). Paiva nevanhu makumi mapfumbamwe nematatu vaifungidzirwa kuti vane chirwere ichi, makumi mashanu nematanhatu akasimbiswa, uye makumi matatu nemanomwe vakafa.(huwandu hwevakafa ne25%). Muna 2012, kumwe kupararira kwechirwere ichi kwakaitika muOrientale Province yeDemocratic Republic of the Congo, ine vanhu 38 vakasimbiswa kuti vabatwa uye 13 vakafa (rufu rwevanhu vakabatwahuwandu hwe34%. Muna Chivabvu 2026, WHO yakazivisa denda reBDBV riripo parizvino muDemocratic Republic of the Congo neUganda idambudziko rehutano hwevanhuInternational Concern (PHEIC). Kusvika musi wa20 Chivabvu, paive nemhosva dzinofungidzirwa mazana maviri nemakumi mana nematanhatu uye makumi masererufu.

Magadzirirwo ehunyanzvi

Kuchengetera	≤-18℃
Nguva yekuzorora	12mwedzi
Rudzi rwemuenzaniso	ropa rose, serum/plasma,oswab yemuto
Ct	≤38
CV	≤5.0%
LoD	300 Makopi/mL
Zvishandiso Zvinoshanda	Rudzi I:Sisitimu yePCR yeABI 7500/7500 Inokurumidza Kushanda Nenguva Yechokwadi, Sisitimu yePCR yeQuantStudio®5 Yenguva Yechokwadi, Sisitimu yePCR yeQuantStudio®7 Yenguva Yechokwadi, Hongshi SLAN-96P/S Fully Automatic Medical PCR Analysis System, LightCycler®480 Real-Time PCR System, Sisitimu yePCR yeHangzhou Bioer FQD-96A Yenguva Yechokwadi, Sisitimu yePCR yeYarui MA-6000 Yenguva Yechokwadi, Sisitimu yePCR yeBioRad CFX96 Real-Time, Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi. Rudzi rwechipiri: Eudemon AIO800-Sisitimu Yekuona Molecular otomatiki (HWTS-AIO800)