15 Mhando dzeHuman Papillomavirus E6/E7 Gene mRNA

Tsanangudzo Pfupi:

Iyi kit yakanangana nekuonekwa kwemhando ye15 high-risk human papillomavirus (HPV) E6/E7 gene mRNA kutaura mazinga mumaseru akaburitswa echibereko chemukadzi.


Product Detail

Product Tags

Product name

HWTS-CC005A-15 Mhando dzeHuman Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)

Epidemiology

Gomarara remuchibereko nderimwe remhando dzegomarara remunhukadzi pasi rose, uye kuitika kwaro kune hukama hwepapillomaviruses (HPV), asi chikamu chidiki cheutachiona hweHPV chinogona kuita gomarara.High-risk HPV inobata cervical epithelial cells uye inogadzira maoncoprotein maviri, E6 uye E7.Iyi puroteni inogona kukanganisa akasiyana-siyana mapuroteni emasero (akadai sebundu suppressor mapuroteni pRB uye p53), kurebesa kutenderera kwesero, kukanganisa DNA synthesis uye genome kugadzikana, uye kukanganisa antiviral uye antitumor immune mhinduro.

Channel

Channel Chikamu Genotype yakaedzwa
FAM HPV Reaction Buffer 1 HPV16,31,33,35,51,52,58
VIC/HEX Munhu β-actin gene
FAM HPV Reaction Buffer 2 HPV 18, 39, 45, 53, 56, 59, 66, 68
VIC/HEX Munhu INS gene

Technical Parameters

Storage Mvura: ≤-18℃
Sherufu-hupenyu 9 mwedzi
Type Type Cervical swab
Ct ≤38
CV <5.0%
LoD 500 Makopi/mL
Zvishandiso Zvishandiso Applied Biosystems 7500 Chaiyo-Nguva PCR SystemApplied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Chaiyo-Nguva PCR Systems

SLAN-96P Chaiyo-Nguva PCR Systems

LightCycler®480 Chaiyo-Nguva PCR system

LineGene 9600 Plus Chaiyo-Nguva PCR Detection System

MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler

BioRad CFX96 Chaiyo-Nguva PCR System

BioRad CFX Opus 96 Chaiyo-Nguva PCR System

Kuyerera Kwebasa

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanira kuitwa zvakanyatsoenderana nemirairo yekushandisa. .Iyo yakakurudzirwa elution vhoriyamu i50μL.Kana sampuli isina kunyatsogayiwa, idzorere kune nhanho 4 yekugaya zvakare.Uye wozoyedza zvinoenderana nemirairo yekushandisa.

Inokurudzirwa kuburitsa reagent: RNAprep Yakachena Mhuka Tissue Yese RNA Extraction Kit (DP431).Kubvisa kunofanirwa kuitwa zvinoenderana nemirairo yekushandisa zvakasimba (Mudanho rechishanu, kaviri kuwanda kweDNaseI kushanda mhinduro, kureva, tora 20μL yeRNase-Yemahara DNaseI (1500U) stock solution muRNase-Mahara centrifuge chubhu, wedzera 60μL yeRDD buffer, uye sanganisa zvinyoro nyoro).Iyo yakakurudzirwa elution vhoriyamu i60μL.Kana sampuli isina kunyatsogayiwa, idzorere kune nhanho yechishanu yekugaya zvakare.Uye wozoyedza zvinoenderana nemirairo yekushandisa.


  • Zvakapfuura:
  • Zvinotevera:

  • Nyora meseji yako pano ugotitumira