Clostridium difficile toxin A/B gene (C.diff)
Product name
HWTS-OT031A Nucleic Acid Detection Kit yeClostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Chitupa
CE
Epidemiology
Clostridium difficile (CD), a gram-positive anaerobic sporogenic Clostridium difficile, ndechimwe chezvirwere zvikuru zvinokonzera nosocomial intestinal infections.Kliniki, inenge 15% ~ 25% yemanyoka ane chokuita neantimicrobial, 50% ~ 75% yeantimicrobial-related colitis uye 95% ~ 100% yepseudomembranous enteritis inokonzerwa neClostridium difficile infection (CDI).Clostridium difficile is conditional pathogen, kusanganisira toxigenic strains uye non-toxigenic strains.
Channel
| FAM | tcdAgene |
| ROX | tcdBgene |
| VIC/HEX | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Zvakananga | shandisa kititi ichi kuona zvimwe zvirwere zvemudumbu zvakaita seEscherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Boka B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, furuwenza, utachiona hwefuruwenza, influenza yevanhu DNA, mhedzisiro yese yakaipa. |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System(FQD-96A,HangzhouBioer tekinoroji) MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
Wedzera 180μL ye lysozyme buffer kune precipitate (dilute lysozyme kusvika 20mg/mL ne lysozyme diluent), pipette kusanganisa zvakanaka, uye gadzira pa 37 ° C kwemaminitsi anopfuura 30. Tora 1.5mL yeRNase / DNase-free centrifuge tube, uye wedzera180μL yekutonga kwakanaka uye kutonga kwakashata mukutevedzana.Wedzera10μL yekutonga kwemukati kune sampuli kuti iongororwe, kutonga kwakanaka, uye kusadzora kwakashata mukutevedzana, uye shandisa iyo Nucleic Acid Kubvisa kana Kuchenesa Reagent (YDP302) neTiangen Biotech (Beijing) Co., Ltd. ndapota nyatsotevera mirairo yekushandisa kune chaiwo matanho.Shandisa DNase/RNase yemahara H2O ye elution, uye yakakurudzirwa elution vhoriyamu i100μL.
Sarudzo 2.
Tora 1.5mL yeRNase/DNase-isina centrifuge chubhu, uye wedzera 200μL yekutonga kwakanaka uye kutonga kwakashata mukutevedzana.Wedzera10μL yekutonga kwemukati kune sampuli kuti iongororwe, kutonga kwakanaka, uye kutonga kwakashata mukutevedzana, uye shandisa iyo Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Kudhirowa kunofanirwa kuitwa zvakanyatsoenderana nekuraira kwekushandisa, uye yakakurudzirwa elution vhoriyamu i80μL.
