Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Product name
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Detection Kit (Fluorescence PCR)
Epidemiology
Carbapenem mishonga inorwisa mabhakitiriya ndeye atypical β-lactam mishonga inorwisa mabhakitiriya uye yakakura kwazvo inorwisa mabhakitiriya uye yakasimba inorwisa mabhakitiriya.Nekuda kwekugadzikana kwayo kune β-lactamase uye yakaderera toxicity, yave imwe yeanonyanya kukosha mishonga inorwisa mabhakitiriya yekurapa kwehutachiona hwakanyanya hwehutachiona.Carbapenems yakagadzikana zvakanyanya kune plasmid-mediated yakawedzera-spectrum β-lactamases (ESBLs), chromosomes, uye plasmid-mediated cephalosporinases (AmpC enzymes).
Channel
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Kudzora Kwemukati | Kudzora Kwemukati |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | Sputum, makoroni akachena, rectal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Zvakananga | a) Iyo kit inoona yakamisikidzwa kambani yakaipa mareferensi, uye mhedzisiro inosangana nezvinodiwa zvezvinoenderana mareferensi. b) Mhedzisiro yemuchinjiko-reactivity bvunzo inoratidza kuti kititi ichi hachina kuita muchinjiko nemamwe hutachiona hwekufema, seKlebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klexytobacter baumannii, Klexytobacinella muCleephilus Aeruginosa. junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, kana sampuli dzine mamwe majini asingaurayiwi nemishonga CTX, mecVA, TEM, etc. c) Anti-kupindira: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin inosarudzwa kuti iongorore kupindira, uye migumisiro inoratidza kuti zvinhu zvinopindira zvakataurwa pamusoro apa hazvina kukanganisa. kusvika pakuonekwa kwe carbapenem resistance genes KPC, NDM, OXA48, OXA23, VIM, uye IMP. |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR Systems QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System (FQD-96A,HangzhouBioer tekinoroji) MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
Inokurudzirwa yekubvisa reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Wedzera 200μL yesaline yakajairika ku iyo thallus inonaya.Matanho anotevera anofanira kutevedzera mirairo yekubvisa, uye yakakurudzirwa elution vhoriyamu ndiyo100μL.
Sarudzo 2.
Inokurudzirwa yekubvisa reagent: Nucleic Acid Extraction or Purification Reagent (YDP302) by Tiangen Biotech (Beijing) Co., Ltd. Kubvisa kunofanirwa kutangwa zvichinyatsoenderana nedanho rechipiri rekuraira kwekushandisa (wedzera 200μL yebuffer GA kune mvura ine thallus. , uye zunza kusvikira thallus yarembera zvachose).Shandisa iyo RNase/DNase yemahara mvura yerution, uye yakakurudzirwa elution vhoriyamu i100μL.
Sarudzo 3.
Inokurudzirwa kuburitsa reagent: Macro & Micro-Test Sample Release Reagent.Sampu yesputum inoda kugezwa nekuwedzera 1mL yesaline yakajairwa kune yataurwa pamusoro yakarapwa thallus precipitate, centrifuged pa13000r/min kwemaminitsi mashanu, uye supernatant inoraswa (chengeta 10-20µL ye supernatant).Kune yakachena colony uye rectal swab, wedzera 50μL yemuenzaniso wekuburitsa reagent zvakananga kune yataurwa pamusoro yakarapwa thallus precipitate, uye nhanho dzinotevera dzinofanirwa kutorwa zvinoenderana nekuraira kwekushandisa.
