Staphylococcus Aureus uye methicillin-inodzivirira staphylococcus aureus (Mrsa / SA)
Chigadzirwa Zita
HWTS-OT062 staphylococccus Aureus-anoramba staphylococccus aureus (Mrsa / sa) nucleic acid yekuona kit (fluorescence pcr)
Chitupa
CE
Epidemiology
Staphylococcus Aureus ndeimwe yeakakosha pathogenic mabhakitiriya ehutachiona. Staphylococcus Aureus (SA) ndezvehunioko uye mumiririri weGram-akanaka mabhakitiriya, ayo anogona kuburitsa akasiyana-siyana emabhonzo uye assasive enzymes. Buttia ine hunhu hwekuparadzira hwakawanda, pathogenicity yakasimba uye huwandu hwakanyanya hwekurwisa. Thermostable nuclease nuclease geru (NUC) chinhu chakachengetedzwa kwazvo chakachengetedzeka chetoleloccus aureus.
Chiteshi
| Nhamo | methicillin-inodzivirira meca gene |
| Rox | Kutonga kwemukati |
| CY5 | Staphylococcus Aureus Nuc |
Technical parameter
| Chengetedza | ≤-18 ℃ & akadzivirirwa kubva pachiedza |
| Shefu-Hupenyu | Mwedzi gumi nemaviri |
| Type Type | Sputum, ganda uye nyoro nyoro tishu kusungwa kwemasampula, uye nasal swab samples |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lod | 1000 CFU / ML STaphylococcus Aureus, 1000 CFU / ML MEMECHICILL-Rosers Buttia. Apo kit ichiona iyo National LOD Reference, 1000 / ml staphylococccus aureus inogona kuonekwa |
| Chokwadi | Iyo yekuyambuka-yekuona bvunzo inoratidza kuti iyi kit yekufunga nedzimwe humwe humwe hutachiona, methicillin staphinosa epidermidis, escherichia auneginosa Acinetabactor Baumannii, Proteus Mirabilis, Enterobacter Cloacae, Strectcocccus pneumoniae, Legidalla Pneumolis, Moraxella Cashrabhis, Moraxella |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva pcr system Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRR Kuongororwa system Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Sarudzo 1.
Macro & micro-test genomic DNA / rna kit (hwts-3019) naJiangsu Macro & micro-test otomatiki acidcrac (HWTS-3006C, HWTS- 3006B). Wedzera 200μL yeyakajairwa saline kune yakagadzirwa seyakagadziriswa, uye matanho anotevera anofanira kubviswa maererano nemirairo, uye vhoriyamu yakakurudzirwa ndeye 80μL.
Sarudzo 2.
Macro & micro-bvunzo sampula sununguko reagent (HWTS-3005-8) naJiangsu Macro & Cocrodha Med-Tech yeChinorina Centrifuge pa13,000R / min kwemaminitsi mashanu, bvisa iyo Supernatant (chengetedza 10-203GE yekunyora), uye tevera mirayiridzo yekubvisa inotevera.
Yakakurudzirwa Reagent Reagent: Nucleic acid yekubvisa kana kuchenesa Reagent (YDP302) naTiangoen Biotech (Beijing) Co, LTD. Iko kubviswa kunofanirwa kuitwa zvakanyatsoenderana maererano nenhanho yechipiri yebhuku rekuraira. Zvinokurudzirwa kushandisa rnase uye dnase-yemahara mvura yekukwevera nehoriyamu ye100μl.
