Kiti yeKuona Staphylococcus Aureus uye Staphylococcus Aureus Inodzivirira Methicillin Nucleic Acid
Zita rechigadzirwa
Kiti yekuona Staphylococcus Aureus uye Staphylococcus Aureus Inodzivirira Methicillin Nucleic Acid (Fluorescence PCR)
Kushandiswa Kwakarongwa
Kit iyi inoshandiswa pakuona staphylococcus aureus uye staphylococcus aureus nucleic acids isingapindi methicillin musampuli dzemapute emunhu, sampuli dzemhino dzinoshandiswa pakurapa uye sampuli dzehutachiona hweganda nenyama yakapfava mu vitro. Kuongororwa kwesampuli yemhino nesampuli yemhino dzinoshandiswa pakurapa kudzivirira nekudzora hutachiona hweMRSA muvarwere vari muchipatara, kusanganisira varwere vanochengetwa zvakanyanya, varwere vanovhiyiwa uye varwere vanochengetwa kwenguva refu; Inogona zvakare kushandiswa pakuongorora varwere vanofungidzirwa kuti vane hutachiona hweStaphylococcus Aureus neMethicillin-Resistant Staphylococcus Aureus; Kana pamwe chete nedzimwe bvunzo dzemurabhoritari dzakadai sema microbial cultures kubatsira pakuongorora hutachiona hweMRSA/SA paganda nenyama yakapfava. Mhedzisiro yebvunzo haishandiswe zvakananga kutungamira kushandiswa kwemishonga inorwisa mabhakitiriya.
Epidemiology
Staphylococcus aureus ndeimwe yemabhakitiriya akakosha anokonzera hutachiona hwepasocomial. Staphylococcus aureus (SA) ndeye staphylococcus uye inomiririra mabhakitiriya eGram-positive, anogona kugadzira chepfu dzakasiyana-siyana uye ma enzymes anorwisa utachiona. Mabhakitiriya ane hunhu hwekupararira kwakafara, hutachiona hwakasimba uye kumhanya kwakanyanya. Thermostable nuclease gene (nuc) ijini rakachengetedzwa zvakanyanya re staphylococcus aureus.
Mumakore achangopfuura, nekuda kwekushandiswa zvakanyanya kwemahormone uye mishonga yekudzivirira muviri uye kushandiswa zvisina kunaka kwemishonga inorwisa mabhakitiriya, hutachiona hwemunosocomial hunokonzerwa neMethicillin-resistant Staphylococcus aureus (MRSA) muStaphylococcus hwave huchiwedzera. Avhareji yehuwandu hweMRSA munyika yese yaive 30.2% muna 2019 muChina. MRSA yakakamurwa kuva MRSA (HA-MRSA) yehutano, MRSA (CA-MRSA) yemunharaunda, uye MRSA (LA-MRSA) yemhuka. CA-MRSA, HA-MRSA, LA-MRSA dzine musiyano mukuru mu microbiology, resistance yemabhakitiriya (semuenzaniso, HA-MRSA inoratidza resistance yakawanda yemishonga kupfuura CA-MRSA) uye hunhu hwekiriniki (semuenzaniso nzvimbo yehutachiona). Zvichienderana nehunhu uhwu, CA-MRSA neHA-MRSA zvinogona kusiyaniswa. Zvisinei, musiyano uripo pakati peCA-MRSA neHA-MRSA uri kudzikira nekuda kwekufamba kwevanhu nguva dzose pakati pezvipatara nenharaunda. MRSA haingopindwe nemishonga yakawanda, haingopindwe nemishonga inorwisa mabhakitiriya inonzi β-lactam chete, asiwo neaminoglycosides, macrolides, tetracyclines uye quinolones kusvika padanho rakasiyana. Kune misiyano mikuru munzvimbo dzakasiyana-siyana muhuwandu hwekusapindwa nemishonga uye maitiro akasiyana. MRSA inogona kupinda mumuviri kuburikidza nemaronda eganda, bvudzi, ropa nezvimwe zvinokonzera hutachiona hwemapundu, uye varwere vane zvirwere zveganda nekutsva vanonyanya kubatwa nehutachiona hweMRSA. Pneumonia ndeimwe yehutachiona hweMRSA hunowanzoonekwa, uye kurapwa kusina kunaka ndicho chikonzero chikuru cherufu rwakawanda.
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤ -18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Sputum, swab yemhino, sampuro yehutachiona hweganda nenyama yakapfava, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Kunyatsojeka | Bvunzo yekuongorora hutachiona hwehutachiona inoratidza kuti kit iyi haina hutachiona hwehutachiona hwehutachiona hwehutachiona hwekufema hwakadai se methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Zvishandiso Zvinoshanda | Rudzi I: Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Rudzi rwechipiri: HWTS-EudemonTM AIO800 |
Kufamba Kwebasa
Zvinodiwa zvekugadzirisa zvinhu nemidziyo asi hazvina kupihwa
Rudzi I
(1) Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) kubva kuJiangsu Macro & Micro-Test Med-Tech Co., Ltd. (Inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2) Muenzaniso wemusanganiswa: 4% NaOH kana Sputasol kubva kuJiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Munyu wakajairika.
Rudzi rwechipiri:
Kit yeMacro & Micro-Test General DNA/RNA Kit (HWTS-3019-8) kubva kuJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Zvinoshandiswa zvinodiwa asi hazvipihwe:Mazano ekushandisa RNase/DNase, Magorovhosi anoraswa, machubhu eEP asina RNase/DNase, zvidimbu zvemachubhu masere zvePCR.
