Staphylococcus Aureus uye Staphylococcus Aureus Inodzivirira Methicillin (MRSA/SA)
Zita rechigadzirwa
HWTS-OT062 Staphylococcus Aureus uye Staphylococcus Aureus Inodzivirira Methicillin (MRSA/SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Chitupa
CE
Epidemiology
Staphylococcus aureus ndeimwe yemabhakitiriya akakosha anokonzera hutachiona hwepasocomial. Staphylococcus aureus (SA) ndeye staphylococcus uye inomiririra mabhakitiriya eGram-positive, anogona kugadzira chepfu dzakasiyana-siyana uye ma enzymes anorwisa utachiona. Mabhakitiriya ane hunhu hwekupararira kwakafara, hutachiona hwakasimba uye kumhanya kwakanyanya. Thermostable nuclease gene (nuc) ijini rakachengetedzwa zvakanyanya re staphylococcus aureus.
Chiteshi
| FAM | jini remecA rinodzivirira methicillin |
| ROX | Kudzora Kwemukati |
| CY5 | jini re staphylococcus aureus nuc |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ & kudzivirirwa kubva kuchiedza |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | sampuli dzemapute, ganda nezvirwere zvenyama yakapfava, uye sampuli dzemushonga wemhino |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL mabhakitiriya anodzivirira methicillin. Kana kit ikaona LoD reference yenyika, 1000/mL staphylococcus aureus inogona kuonekwa |
| Kunyatsojeka | Bvunzo yekuongorora hutachiona hwehutachiona inoratidza kuti kit iyi haina hutachiona hwehutachiona hwehutachiona hwehutachiona hwekufema hwakadai se methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Wedzera 200µL yemunyu wakajairika kune precipitate yakagadziriswa, uye matanho anotevera anofanira kutorwa zvichienderana nemirairo, uye huwandu hwekudzikisa hunokurudzirwa i80µL.
Sarudzo yechipiri.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Wedzera 1mL yemunyu wakajairika kumushonga mushure mekusuka nemunyu wakajairika, wobva wasanganisa zvakanaka. Centrifuge pa13,000r/min kwemaminitsi mashanu, bvisa mushonga wacho (chengeta 10-20µL yemunyu wakanyanya), uye tevera mirairo yekuburitsa mushonga uyu mushure mezvo.
Chinorayirwa kubvisa: Nucleic Acid Extraction kana Purification Reagent (YDP302) naTiangen Biotech (Beijing) Co., Ltd. Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nedanho rechipiri rebhuku remirayiridzo. Zvinokurudzirwa kushandisa mvura isina RNase neDNase kuti ibvise mvura ine huwandu hwe100µL.
