Mycobacterium Tuberculosis Isoniazid Resistance Mutation

Tsanangudzo Pfupi:

Iyi kit inokodzera kuongororwa kwehutano hwenzvimbo huru dzekuchinja mumasampumu emunhu akaunganidzwa kubva kuTubercle bacillus positive varwere vanotungamira mycobacterium tuberculosis isoniazid resistance: InhA promotioner region -15C>T, -8T>A, -8T>C;AhpC mutsigiri dunhu -12C>T, -6G>A;homozygous mutation yeKatG 315 codon 315G>A, 315G>C .


Product Detail

Product Tags

Product name

HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Melting Curve)

Epidemiology

Mycobacterium tuberculosis, kwenguva pfupi seTubercle bacillus (TB), utachiona hwepathogenic hunokonzera tibhii.Parizvino, mishonga yemutsara wekutanga inorwisa tibhii inowanzoshandiswa inosanganisira isoniazid, rifampicin nehexambutol, nezvimwewo. Mishonga yepiriviri inorwisa tuberculosis inosanganisira fluoroquinolones, amikacin uye kanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa inonzi linezolid, bedaquiline nedelamani, nezvimwewo. .Zvisinei, nekuda kwekusashandiswa zvisirizvo kwemishonga inorwisa tibhii uye hunhu hwesero rusvingo rwemycobacterium tuberculosis, mycobacterium tuberculosis inokura kurwisa zvinodhaka kumishonga inorwisa tibhii, izvo zvinounza matambudziko akakomba mukudzivirira uye kurapwa kwetibhii.

Channel

FAM MP nucleic acid
ROX

Kudzora Kwemukati

Technical Parameters

Storage

≤-18℃

Sherufu-hupenyu 12 mwedzi
Type Type sputum
CV ≤5%
LoD Muganho wekuona wemusango-mhando isoniazid inoshingirira mabhakitiriya i2x103 mabhakitiriya/mL, uye muganho wekuona mabhakitiriya anoshanduka ndeye 2x103 mabhakitiriya/mL.
Zvakananga a.Iko hakuna kuita kwemuchinjikwa pakati pejenome remunhu, mamwe asingatuberculous mycobacteria uye pneumonia pathogens akaonekwa nekiti iyi.b.Nzvimbo dzekuchinja dzemamwe majini asingarambike nemishonga mune yemusango-rudzi rweMycobacterium tuberculosis, senge nharaunda inotaridza kurwisa kwerifampicin rpoB gene, zvakaonekwa, uye mhedzisiro yebvunzo yakaratidza kusapokana neisoniazid, zvichiratidza kuti hapana chinjika reactivity.
Zvishandiso Zvishandiso SLAN-96P Chaiyo-Nguva PCR SystemsBioRad CFX96 Chaiyo-Nguva PCR Systems

LightCycler480® Chaiyo-Nguva PCR System

Kuyerera Kwebasa

Kana ukashandisa iyo Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) naJiangsu Macro & Micro-Test. Med-Tech Co., Ltd. yekubvisa, wedzera mazana maviriμL yekutonga kwakashata uye yakagadziriswa sputum sampuli kuti iongororwe mukutevedzana, uye wedzera gumiμL yekutonga kwemukati zvakaparadzana mukati mekutonga kwakashata, yakagadziriswa sputum sampu kuti iongororwe, uye matanho anotevera anofanirwa kuitwa zvakanyatsoenderana nemirairo yekubvisa.Iyo yakabudiswa sampuli yevhoriyamu ndeye 200μL, uye yakakurudzirwa elution vhoriyamu i100μL.


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