Staphylococcus Aureus uye Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Product name
HWTS-OT062 Staphylococcus Aureus uye Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)
Chitupa
CE
Epidemiology
Staphylococcus aureus imwe yeakakosha pathogenic mabhakitiriya ehutachiona hweNosocomial.Staphylococcus aureus (SA) ndeye staphylococcus uye inomiririra Gram-positive bacteria, iyo inogona kubereka zvakasiyana-siyana zvehupfu uye invasive enzymes.Iwo mabhakitiriya ane hunhu hwekugovera kwakakura, hwakasimba pathogenicity uye yakakwirira yekupikisa mwero.Thermostable nuclease gene (nuc) ijeni rakachengetedzwa zvakanyanya re staphylococcus aureus.
Channel
| FAM | Methicillin-resistant mecA gene |
| ROX | Kudzora Kwemukati |
| CY5 | staphylococcus aureus nuc gene |
Technical Parameters
| Storage | ≤-18 ℃ & yakachengetedzwa kubva kuchiedza |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | sputum, ganda uye zvinyoro zvinyoro zvehutachiona hwehutachiona, uye masampuri emumhino |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL methicillin-resistant mabhakitiriya.Kana iyo kiti yaona iyo yenyika LoD referensi, 1000/mL staphylococcus aureus inogona kuonekwa. |
| Zvakananga | Iyo yekuyambuka-tarisiro yeyekuti iyi kit iyi yekufunga nedzimwe nzira dzinoponesa, methicillo nii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR Systems Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd. inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Wedzera 200µL yesaline yakajairwa kumvura yakagadziriswa, uye nhanho dzinotevera dzinofanirwa kutorwa zvinoenderana nemirairo, uye yakakurudzirwa elution vhoriyamu i80µL.
Sarudzo 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Wedzera 1mL yesaline yakajairika kune inonaya mushure mekugeza nesaline yakajairika, wozosanganisa zvakanaka.Centrifuge pa13,000r/min kwemaminetsi mashanu, bvisa supernatant (chengeta 10-20µL ye supernatant), uye tevera mirairo yekuzochera kunotevera.
Inokurudzirwa yekubvisa reagent: Nucleic Acid Extraction kana Purification Reagent (YDP302) neTiangen Biotech (Beijing) Co.,Ltd.Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nedanho 2 rebhuku rekuraira.Zvinokurudzirwa kushandisa RNase uye DNase-isina mvura yerution ine huwandu hwe100µL.
