Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance
Product name
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance Detection Kit (Melting Curve)
Chitupa
CE
Epidemiology
Mycobacterium tuberculosis, munguva pfupi seTubercle bacillus, TB, utachiona hwepathogenic hunokonzera tibhii.Parizvino, mishonga yemutsara wekutanga inorwisa tibhii inowanzoshandiswa inosanganisira isoniazid, rifampicin nehexambutol, nezvimwewo. Mishonga yepiriviri inorwisa tuberculosis inosanganisira fluoroquinolones, amikacin uye kanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa inonzi linezolid, bedaquiline nedelamani, nezvimwewo. .Zvisinei, nekuda kwekusashandiswa zvisirizvo kwemishonga inorwisa tibhii uye hunhu hwesero rusvingo rwemycobacterium tuberculosis, mycobacterium tuberculosis inokura kurwisa zvinodhaka kumishonga inorwisa tibhii, izvo zvinounza matambudziko akakomba mukudzivirira uye kurapwa kwetibhii.
Rifampicin yakashandiswa zvakanyanya mukurapa varwere vepulmonary tuberculosis kubva mukupera kwema1970, uye ine simba rakakura.Yakave sarudzo yekutanga kupfupisa chemotherapy yevarwere vepulmonary tuberculosis.Kuramba kweRifampicin kunonyanya kukonzerwa nekushandurwa kwejene rerpoB.Kunyange zvazvo mishonga mitsva yekurwisa tibhii iri kuramba ichibuda, uye kushanda kwekiriniki kwevarwere vepulmonary tuberculosis kwakaramba kuchiwedzera, kuchine kushaikwa kwemishonga inorwisa tibhii, uye chiitiko chekushandisa zvinodhaka zvisina musoro mumakiriniki zvakanyanyisa.Zviri pachena kuti Mycobacterium tuberculosis muvarwere vane pulmonary tuberculosis haigoni kuurayiwa zvachose panguva yakakodzera, iyo inoguma inotungamirira kumadhigirii akasiyana-siyana ekudzivirira zvinodhaka mumuviri wemurwere, inowedzera nguva yechirwere, uye inowedzera ngozi yekufa kwemurwere.
Channel
| Channel | Zviteshi uye Fluorophores | Reaction Buffer A | Reaction Buffer B | Reaction Buffer C |
| FAM Channel | Mutori wenhau: FAM, Quencher: Hapana | rpoB 507-514 | rpoB 513-520 | 38KD uye IS6110 |
| CY5 Channel | Mutori wenhau: CY5, Quencher: Hapana | rpoB 520-527 | rpoB 527-533 | / |
| HEX (VIC) Channel | Mutori wenhau: HEX (VIC), Quencher: Hapana | Kudzora mukati | Kudzora mukati | Kudzora mukati |
Technical Parameters
| Storage | ≤-18℃ Murima |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | Sputum |
| CV | ≤5.0% |
| LoD | mycobacterium tuberculosis 50 mabhakitiriya / mL rifampicin-resistant mhando yemusango: 2x103utachiona/mL homozygous mutant: 2x103utachiona/mL |
| Zvakananga | Inoona mhuka yemusango mycobacterium tuberculosis uye nzvimbo dzekuchinja kwemamwe majini ekuramba zvinodhaka akadai se katG 315G>C\A, InhA-15C> T, mibairo yebvunzo inoratidza kusaramba rifampicin, zvinoreva kuti hapana kuyambuka-reactivity. |
| Zvishandiso Zvinoshanda: | SLAN-96P Chaiyo-Nguva PCR Systems BioRad CFX96 Chaiyo-Nguva PCR System LightCycler480® Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Kana ukashandisa iyo Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (iyo inogona kushandiswa neMacro & Micro-Test Otomatiki. Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) kana Macro & Micro-Test Viral DNA/RNA Column(HWTS-3022-50) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. 200μL yePositive Control, kudzora kwakashata uye yakagadziriswa sputum sampu kuti iongororwe munhevedzano, uye wedzera 10μL yekutonga kwemukati zvakasiyana muPositive Control, kudzora kwakashata uye kugadziridzwa sputum sampu kuti iongororwe, uye nhanho dzinotevera dzinofanira kuitwa zvakanyatsonaka. maererano nemirayiridzo yekuchera.Iyo yakabviswa sampuli vhoriyamu i200μL, uye yakakurudzirwa elution vhoriyamu i100μL.
