Mycobacterium Tuberculosis Isoniazid Resistance Mutation
Product name
HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, kwenguva pfupi seTubercle bacillus (TB), utachiona hwepathogenic hunokonzera tibhii.Parizvino, mishonga yemutsara wekutanga inorwisa tibhii inowanzoshandiswa inosanganisira isoniazid, rifampicin nehexambutol, nezvimwewo. Mishonga yepiriviri inorwisa tuberculosis inosanganisira fluoroquinolones, amikacin uye kanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa inonzi linezolid, bedaquiline nedelamani, nezvimwewo. .Zvisinei, nekuda kwekusashandiswa zvisirizvo kwemishonga inorwisa tibhii uye hunhu hwesero rusvingo rwemycobacterium tuberculosis, mycobacterium tuberculosis inokura kurwisa zvinodhaka kumishonga inorwisa tibhii, izvo zvinounza matambudziko akakomba mukudzivirira uye kurapwa kwetibhii.
Channel
| FAM | MP nucleic acid |
| ROX | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | sputum |
| CV | ≤5% |
| LoD | Muganho wekuona wemusango-mhando isoniazid inoshingirira mabhakitiriya i2x103 mabhakitiriya/mL, uye muganho wekuona mabhakitiriya anoshanduka ndeye 2x103 mabhakitiriya/mL. |
| Zvakananga | a.Iko hakuna kuita kwemuchinjikwa pakati pejenome remunhu, mamwe asingatuberculous mycobacteria uye pneumonia pathogens akaonekwa nekiti iyi.b.Nzvimbo dzekuchinja dzemamwe majini asingarambike nemishonga mune yemusango-rudzi rweMycobacterium tuberculosis, senge nharaunda inotaridza kurwisa kwerifampicin rpoB gene, zvakaonekwa, uye mhedzisiro yebvunzo yakaratidza kusapokana neisoniazid, zvichiratidza kuti hapana chinjika reactivity. |
| Zvishandiso Zvishandiso | SLAN-96P Chaiyo-Nguva PCR SystemsBioRad CFX96 Chaiyo-Nguva PCR Systems LightCycler480® Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Kana ukashandisa iyo Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) naJiangsu Macro & Micro-Test. Med-Tech Co., Ltd. yekubvisa, wedzera mazana maviriμL yekutonga kwakashata uye yakagadziriswa sputum sampuli kuti iongororwe mukutevedzana, uye wedzera gumiμL yekutonga kwemukati zvakaparadzana mukati mekutonga kwakashata, yakagadziriswa sputum sampu kuti iongororwe, uye matanho anotevera anofanirwa kuitwa zvakanyatsoenderana nemirairo yekubvisa.Iyo yakabudiswa sampuli yevhoriyamu ndeye 200μL, uye yakakurudzirwa elution vhoriyamu i100μL.
