Kuchinja kweMycobacterium Tuberculosis INH
Zita rechigadzirwa
HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, iyo inonzi Tubercle bacillus (TB), ndiyo bhakitiriya inokonzera chirwere cherurindi. Parizvino, mishonga yekutanga inoshandiswa zvakanyanya yekurwisa TB inosanganisira INH, rifampicin nehexambutol, nezvimwewo. Mishonga yechipiri yekurwisa TB inosanganisira fluoroquinolones, amikacin nekanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa ndeye linezolid, bedaquiline nedelamani, nezvimwewo. Zvisinei, nekuda kwekushandiswa zvisirizvo kwemishonga yekurwisa TB uye hunhu hwemadziro emasero e mycobacterium tuberculosis, mycobacterium tuberculosis inoita kuti mishonga yekurwisa TB isashande, izvo zvinounza matambudziko makuru mukudzivirira nekurapa TB.
Chiteshi
| FAM | asidhi ye nucleic yeMP |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | pfapfaidzo |
| CV | ≤5% |
| LoD | Muganho wekuona mabhakitiriya emhando yeINH emusango i2x103 mabhakitiriya/mL, uye muganho wekuona mabhakitiriya akachinja i2x103 mabhakitiriya/mL. |
| Kunyatsojeka | a. Hapana kusangana pakati pemajini evanhu, mamwe mabhakitiriya asina TB uye pneumonia anowanikwa nekit iyi.b. Nzvimbo dzekuchinja kwemamwe majini asingapindi mishonga muMycobacterium tuberculosis yemhando yemusango, dzakadai senzvimbo inoona kusapindirana kwerifampicin rpoB gene, dzakaonekwa, uye mhinduro dzebvunzo dzakaratidza kusapindirana neINH, zvichiratidza kusapindirana kwemasero. |
| Zvishandiso Zvinoshanda | Masisitimu ePCR eSLAN-96P Enguva YechokwadiBioRad CFX96 Real-Time PCR SystemsLightCycler480®Sisitimu yePCR Yenguva Yechokwadi |
Kufamba Kwebasa
Kana ukashandisa Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. kuti ubvise tsvina, wedzera 200μL yenegative control uye processed sputum sample kuti iongororwe zvakatevedzana, uye wedzera 10μL yeinternal control yoga mu negative control, processed sputum sample kuti iongororwe, uye matanho anotevera anofanira kuitwa zvakanyatsonaka zvichienderana nemirairo yekubvisa tsvina. Vhoriyamu yesample yakabviswa i200μL, uye vhoriyamu yekudzikisa inokurudzirwa i100μL.
