Furuwenza A Virus Universal/H1/H3
Zita rechigadzirwa
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit (Fluorescence PCR)
Epidemiology
Utachiona hwefuruwenza imhando yeOrthomyxoviridae. Ihutachiona hunotyisidzira hutano hwevanhu zvakanyanya. Hunogona kutapurira munhu wacho zvakanyanya. Denda remwaka rinobata vanhu vanosvika mamiriyoni mazana matanhatu pasi rose uye rinokonzera kufa kwevanhu vanosvika 250,000 kusvika 500,000, uye hutachiona hwefuruwenza A ndihwo hunonyanya kukonzera kutapurirana nerufu. Utachiona hwefuruwenza A iRNA ine tambo imwe chete isina tambo. Zvichienderana nehemagglutinin yayo yepamusoro (HA) ne neuraminidase (NA), HA inogona kukamurwa kuita mhando gumi nematanhatu, NA Yakakamurwa kuita mhando pfumbamwe. Pakati pemavhairasi efuruwenza A, mhando dzemavhairasi efuruwenza anogona kutapurirana vanhu zvakananga ndeidzi: A H1N1, H3N2, H5N1, H7N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 neH10N8. Pakati pawo, mhando dzeH1 neH3 dzinotapurirana zvakanyanya, uye dzinonyanya kukodzera kutariswa.
Chiteshi
| FAM | furuwenza A universal type nucleic acid |
| VIC/HEX | furuwenza yerudzi rweH1 virus nucleic acid |
| ROX | furuwenza yerudzi rweH3 virus nucleic acid |
| CY5 | kudzora kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | swab yemumhino |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/μL |
| Kunyatsojeka | Hapana kusangana kwezvirwere zvakasiyana-siyana pamwe chete nezvimwe zviratidzo zvekufema zvakaita seInfluenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, nezvimwewo uye human genomic DNA. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Kubvisa kana Kuchenesa Nucleic Acid Reagent (YDP315-R) neTiangen Biotech (Beijing) Co., Ltd. Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakatorwa i140μL, uye vhoriyamu yekudzikisa inokurudzirwa i60μL.
Sarudzo yechipiri.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakabviswa i200μL, uye vhoriyamu yekudzikisa inokurudzirwa i80μL.
