Zaire Ebola Virus
Product name
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Utachiona hweEbola ndehweFiloviridae, inova hutachiona hweRNA husina kupatsanurwa.Utachiona hurefu hurefu hurefu hwevirion hunosvika 1000nm uye dhayamita ingangoita 100nm.Ebola virus genome is an unsegmented negative-strand RNA ine saizi ye18.9kb, encoding 7 structural proteins uye 1 non-structural protein.Ebola virus inogona kukamurwa kuita mhando dzakaita seZaire, Sudan, Bundibugyo, Tai Forest uye Reston.Pakati pavo, mhando yeZaire neSudan zvinonzi zvakakonzera kufa kwevanhu vakawanda neutachiona.EHF (Ebola Hemorrhagic Fever) chirwere chinotapukira chinotapukira chinokonzerwa neutachiona hweEbola.Vanhu vanonyanya kutapukirwa nekubatana nemvura yemumuviri, kuvharwa uye tsvina yevarwere kana mhuka dzine utachiona, uye zviratidzo zvekiriniki zvinonyanya kubuda kupisa, kubuda ropa uye kukanganisa kwenhengo dzakawanda.EHF ine mwero wepamusoro wekufa kwe50% -90%.
Channel
| FAM | MP nucleic acid |
| ROX | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 9 mwedzi |
| Type Type | Fresh serum, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Makopi/μL |
| Zvakananga | Shandisa makiti kuti uedze kambani yakaipa mareferensi, mhedzisiro inosangana nezvinodiwa. |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System(FQD-96A, Hangzhou Bioer tekinoroji) MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Chaiyo-Nguva PCR System, uye BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
Inokurudzirwa kuchera reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction kana Purification Reagent (YDP315-R) neTiangen Biotech (Beijing) Co.,Ltd.Inofanira kutorwa zvakanyatsoenderana nemirairo, uye yakakurudzirwa yekuwedzera vhoriyamu yesampuli ndeye 140μL uye yakakurudzirwa elution vhoriyamu i60μL.
Sarudzo 2.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). inofanira kutorwa maererano nemirairo.Iyo yekubvisa sampuli vhoriyamu i200μL, uye yakakurudzirwa elution vhoriyamu i80μL.
Sarudzo 3.
Zvinokurudzirwa kutora reagents: Nucleic Acid Extraction Reagent (1000020261) uye High Throughput Automated Sample Preparation System (MGISP-960) neBGI inofanira kutorwa maererano nemirairo.Vhoriyamu yekubvisa i160μL, uye yakakurudzirwa elution vhoriyamu i60μL.
