Kuumbwa kweMajini eCYP2C9 evanhu neVKORC1
Zita rechigadzirwa
HWTS-GE014A-Human CYP2C9 uye VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)
Chitupa
CE/TFDA
Epidemiology
Warfarin mushonga unorwisa ropa rinoita kuti ropa riome unoshandiswa zvakanyanya mukurapa pari zvino, uyo unonyanya kuitirwa kudzivirira nekurapa zvirwere zve thromboembolic. Zvisinei, warfarin ine mukana mudiki wekurapa uye inosiyana zvikuru pakati pemarudzi akasiyana nevanhu. Nhamba dzakaratidza kuti musiyano wemuyero wakagadzikana muvanhu vakasiyana unogona kuva kanopfuura makumi maviri. Kubuda ropa kwakashata kunoitika mu15.2% yevarwere vanotora warfarin gore rega rega, avo 3.5% vanobuda ropa rinouraya. Zvidzidzo zve Pharmacogenomic zvakaratidza kuti genetic polymorphism ye target enzyme VKORC1 uye metabolic enzyme CYP2C9 ye warfarin chinhu chakakosha chinokanganisa musiyano wemuyero we warfarin. Warfarin inodzivirira chaiyo ye vitamin K epoxide reductase (VKORC1), uye nokudaro inodzivirira kugadzirwa kwe clotting factor inosanganisira vitamin K uye inopa anticoagulation. Zvidzidzo zvakawanda zvakaratidza kuti gene polymorphism yeVKORC1 promoter ndiyo inonyanya kukanganisa mutsauko werudzi uye wega wega mumuyero unodiwa we warfarin. Warfarin inogadziriswa neCYP2C9, uye mishonga yayo inochinja-chinja inononoka kushandiswa kwewarfarin. Vanhu vanoshandisa warfarin vane njodzi yakakura (yakapetwa kaviri kusvika katatu) yekubuda ropa pakutanga kwekushandisa.
Chiteshi
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Ropa idzva reEDTA risingapindi ropa |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Kunyatsojeka | Hapana kubatanidzwa kwemasero pamwe chete nemamwe magene evanhu akafanana (gene remunhu reCYP2C19, gene remunhu reRPN2); kuchinja kweCYP2C9*13 neVKORC1 (3730G>A) kunze kwenzvimbo yekuona yekit iyi. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Zvinokurudzirwa kuti munhu abvise reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
