Mhando makumi maviri nemapfumbamwe dzezvirwere zvinofema zvinosanganiswa neNucleic Acid
Zita rechigadzirwa
HWTS-RT160 -29 Mhando dzeUtachiona hweKufema Combined Nucleic Acid Detection Kit
Epidemiology
Utachiona hwemunzira yekufema ndicho chirwere chinowanzoitika muvanhu, chinogona kuitika chero muvanhurume kana muvanhukadzi, zera uye nzvimbo. Ndicho chimwe chezvikonzero zvikuru zvekurwara nekufa kwevanhu pasi rose[1]. Utachiona hunowanzoonekwa mukufema hunosanganisira coronavirus itsva, hutachiona hweInfluenza A, hutachiona hweInfluenza B, hutachiona hweRespiratory syncytial, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, uye Streptococcus pneumoniae, nezvimwewo[2,3]. Zviratidzo nezviratidzo zvinokonzerwa nehutachiona hwekufema zvakafanana, asi nzira dzekurapa, kushanda zvakanaka uye nzira yehutachiona hunokonzerwa nehutachiona hwakasiyana zvakasiyana[4,5]. Parizvino, nzira huru dzinoshandiswa mumarabhoritari kuona utachiona hwekufema hwataurwa pamusoro apa dzinosanganisira: kupatsanurwa kwehutachiona, kuonekwa kweantigen uye kuonekwa kwe nucleic acid, nezvimwewo. Kit iyi inoona uye inoziva viral nucleic acids muvanhu vane zviratidzo nezviratidzo zvehutachiona hwekufema, nekutarisa mavhairasi efuruwenza ne coronaviruses, uye inosanganiswa nemimwe mhedzisiro yerabhoritari kupa rubatsiro pakuongorora hutachiona hwekufema. Mhedzisiro yakaipa haibvisi hutachiona hwekufema uye haifanirwe kushandiswa senzira chete yekuongorora, kurapwa, kana dzimwe sarudzo dzekutarisira. Mhedzisiro yakanaka haigone kubvisa hutachiona hwebhakitiriya kana hutachiona hwakasanganiswa nemamwe mavhairasi huri kunze kwezviratidzo zvekuyedza. Vashandi vekuyedza vanofanirwa kunge vakadzidziswa nehunyanzvi mukuwedzera majini kana kuona molecular biology, uye vane hunyanzvi hwekuita bvunzo dzakakodzera. Rabhoritari inofanira kunge iine nzvimbo dzekudzivirira hutachiona hwehutachiona uye nzira dzekudzivirira.
Magadzirirwo ehunyanzvi
| Kuchengetera | -18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Chinoputira pahuro |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Makopi 200/mL |
| Kunyatsojeka | Mhedzisiro yebvunzo ye cross-reactivity yakaratidza kuti pakanga pasina kusangana pakati pe kit iyi neCytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii, uye human genomic nucleic acids. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu 7500 Masisitimu EPCR Enguva Yechokwadi,Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Nguva Yechokwadi, QuantStudio®Masisitimu mashanu ePCR eNguva Yechokwadi, Masisitimu ePCR eSLAN-96P eNguva Yechokwadi (Hongshi Medical Technology Co., Ltd.), Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time, Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer), MA-6000 Chaiyo-Nguva Yese Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.), Sisitimu yePCR yeBioRad CFX96 Real-Time, Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi. |
Kufamba Kwebasa
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), uye Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (inogona kushandiswa neEudemonTM AIO800 (HWTS-EQ007)) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Vhoriyamu yemuenzaniso yakatorwa i200μL uye vhoriyamu yekudzikisa inokurudzirwa i150μL.
