Zika Virus
Product name
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Chitupa
CE
Epidemiology
Zika virus ndeyemhando yeFlaviviridae, ihutachiona hweRNA ine tambo imwe chete ine dhayamita ye40-70nm. Iine hamvuropu, ine 10794 nucleotides, uye encodes 3419 amino acids. Zvinoenderana neiyo genotype, yakakamurwa kuva Africa mhando uye Asia mhando. Chirwere cheZika virus chirwere chinotapukira chinokonzerwa nehutachiona hweZika, icho chinonyanya kutapurirwa kuburikidza nekurumwa kweAedes aegypti mosquito. Zvimiro zvekiriniki zvinonyanya kupisa kupisa, kuputika, arthralgia kana conjunctivitis, uye haiwanzouraya. Maererano neWorld Health Organization, neonatal microcephaly uye Guillain-Barre syndrome (Guillain-Barré syndrome) inogona kusanganiswa neZika virus infection.
Channel
| FAM | Zika virus nucleic acid |
| ROX | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤30 ℃ & yakachengetedzwa kubva kuchiedza |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | serum itsva |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Makopi/mL |
| Zvakananga | Shandisa kit kuona serum samples ine Zika virus negative, uye mhedzisiro yacho yakaipa. Kupindira kwekuongororwa kwemigumisiro inoratidza kuti kana huwandu hwebilirubin mu serum isingapfuuri 168.2μmol / ml, hemoglobin concentration inobudiswa nehemolysis haisi kupfuura 130g / L, ropa re lipid concentration haisi kupfuura 65mmol / ml, iyo yose yeIgG muserum haisi kupfuura 5mg / mL, hapana hutachiona hweZika kana chikungue hutachiona. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus uye human genomic serum samples inosarudzwa pamuchinjikwa-reactivity bvunzo, uye mhedzisiro inoratidza kuti hapana kupindirana pakati pekiti iyi uye hutachiona hwataurwa pamusoro apa. |
| Zvishandiso Zvishandiso | ABI 7500 Chaiyo-Nguva PCR SystemsABI 7500 Inokurumidza Chaiyo-Nguva PCR Systems Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Kubvisa kana Kuchenesa Reagent (YDP315-R) neTiangen Biotech(Beijing) Co.,Ltd.The extractioninofanira kutorwa maererano nemirairo yekuchera, uye yakakurudzirwa yekuwedzera vhoriyamu i140 μL uye yakakurudzirwa elution vhoriyamu i60 μL.
Sarudzo 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Iko kubviswa kunofanira kutorwa maererano nemirairo. Iyo yekubvisa sampuli vhoriyamu i200 μL, uye yakakurudzirwa elution vhoriyamu i80μL.
