Virusi reZika
Zita rechigadzirwa
HWTS-FE002 Kiti yeKuona Zvirwere zveNucleic Acid yeZika Virus (Fluorescence PCR)
Chitupa
CE
Epidemiology
Virusi reZika riri murudzi rweFlaviviridae, ivhairasi reRNA rine tambo imwe chete rine dhayamita ye40-70nm. Rine kaburi, rine manucleotides 10794, uye rine maamino acids 3419. Zvichienderana nerudzi rwejenomu, rakakamurwa kuva rudzi rweAfrica nerudzi rweAsia. Chirwere cheZika chirwere chinotapukira chinozviparadzanisa chinokonzerwa nevhairasi reZika, iro rinonyanya kutapuriranwa kuburikidza nekurumwa neumhutu hweAedes aegypti. Zviratidzo zvekiriniki zvinonyanya kuve fivha, mapundu, arthralgia kana conjunctivitis, uye hazviwanzo kuuraya. Zvichienderana neWorld Health Organization, neonatal microcephaly uye Guillain-Barre syndrome (Guillain-Barré syndrome) zvinogona kunge zvakabatana nehutachiona hweZika.
Chiteshi
| FAM | Asidhi yeNucleic Acid yeZika virus |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤30℃ & kudzivirirwa kubva kuchiedza |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | seramu itsva |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Makopi 500/mL |
| Kunyatsojeka | Shandisa kit iyi kuona sampuli dzemuropa dzine Zika virus negative, uye mhedzisiro yacho inenge isina. Mhedzisiro yeongororo yekupindira inoratidza kuti kana huwandu hwebilirubin muropa husingapfuure 168.2μmol/ml, huwandu hwehemoglobin hunogadzirwa nehemolysis haupfuure 130g/L, huwandu hwemafuta muropa haupfuure 65mmol/ml, huwandu hwese hweIgG muropa haupfuure 5mg/mL, hapana mhedzisiro pahutachiona hwedengue, kuonekwa kwehutachiona hweZika kana chikungunya. Virusi reHepatitis A, hutachiona hweHepatitis B, hutachiona hweHepatitis C, hutachiona hweHerpes, hutachiona hweEastern equine encephalitis, Hantavirus, hutachiona hweBunya, hutachiona hweWest Nile uye sampuli dze serum dze genomic dzevanhu dzinosarudzwa kuti dziitwe bvunzo ye cross-reactivity, uye mhedzisiro yacho inoratidza kuti hapana cross reaction pakati pekit iyi nehutachiona hwataurwa pamusoro apa. |
| Zvishandiso Zvinoshanda | Masisitimu eABI 7500 PCR Enguva YechokwadiMasisitimu ePCR eABI 7500 Anokurumidza Kushanda Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Kiti Yediki yeQIAamp Viral RNA (52904), Kubvisa Nucleic Acid kana Reagent Yekuchenesa (YDP315-R) naTiangen Biotech(Beijing) Co.,Ltd.Kubviswainofanira kutorwa zvichienderana nemirayiridzo yekubviswa, uye huwandu hunokurudzirwa hwekubviswa i140 μL uye huwandu hunokurudzirwa hwekugadziriswa i60 μL.
Sarudzo yechipiri.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kubviswa kunofanirwa kutorwa zvichienderana nemirairo. Vhoriyamu yekubvisa i200 μL, uye vhoriyamu yekudzikisa inokurudzirwa i80μL.
