Clostridium difficile toxin A/B gene (C.diff)
Zita rechigadzirwa
HWTS-OT031A Nucleic Acid Detection Kit yeClostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Chitupa
CE
Epidemiology
Clostridium difficile (CD), chirwere chinorwisa utachiona chinonzi anaerobic sporogenic chinonzi Clostridium difficile, chimwe chezvirwere zvikuru zvinokonzera hutachiona hwemudumbu hwemudumbu. Panyaya dzekurapa, inenge 15% ~ 25% yemanyoka ane chekuita nehutachiona, 50% ~ 75% yechirwere checolitis chinokonzerwa nehutachiona uye 95% ~ 100% ye pseudomembranous enteritis zvinokonzerwa nehutachiona hweClostridium difficile (CDI). Clostridium difficile ihutachiona hunokonzera zvirwere, hunosanganisira zvirwere zvinouraya utachiona uye zvirwere zvisina chepfu.
Chiteshi
| FAM | tcdAmajini |
| ROX | tcdBmajini |
| VIC/HEX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | chigaro |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Kunyatsojeka | Shandisa kit iyi kuona zvimwe zvirwere zvemudumbu zvakaita seEscherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus uye human genomic DNA, mhedzisiro yacho yese haina kunaka. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A，HangzhouTekinoroji yeBioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Wedzera 180μL ye lysozyme buffer kune precipitate (sanganisa lysozyme kusvika 20mg/mL ne lysozyme diluent), pipette kuti isangane zvakanaka, wobva wagadzirisa pa37°C kwemaminitsi anopfuura 30. Tora 1.5mL yeRNase/DNase-free centrifuge tube, wobva wawedzera180μL yekutonga kwakanaka uye kutonga kusina kunaka zvakatevedzana. Wedzera10μL yekudzora kwemukati kumuenzaniso uchaedzwa, kudzora kwakanaka, uye kudzora kusina kunaka zvakatevedzana, uye kushandisa Nucleic Acid Extraction kana Purification Reagent (YDP302) neTiangen Biotech (Beijing) Co., Ltd. kuti muwane muenzaniso wekubvisa DNA, uye ndapota teverai mirairo yekushandisa pamatanho chaiwo. Shandisai DNase/RNase isina H2O yekudzikisa, uye huwandu hwekudzikisa hunokurudzirwa i100μL.
Sarudzo yechipiri.
Tora 1.5mL yechubhu yeRNase/DNase isina centrifuge, wobva wawedzera 200μL ye positive control uye negative control zvakatevedzana.10μL yekudzora kwemukati kune sampuro inofanira kuongororwa, kudzora kwakanaka, uye kudzora kusina kunaka zvakatevedzana, uye shandisa Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kubvisa kunofanirwa kuitwa zvichienderana nemirairo yekushandisa, uye huwandu hwekudzikisa hunokurudzirwa i80μL.
