Clostrium videicle toxin a / b gene (c.D.Diff)
Chigadzirwa Zita
HWTS-OT031A Nucleic Acid Kuona Kitha yeClostrium Diartile toxin A / B Gene (CGRE (C.Diff)
Chitupa
CE
Epidemiology
CLOSTrifsial uddale (CD), gram-yakanaka anaerobic sporgenic clostigenic clostrilies, ndeimwe yematehwe makuru anokonzeresa hosha yakaipa. Clinically, anenge 15% ~ 25% yeAntimicrobial-inoenderana neLarrhea, ine makumi manomwe neshanu muzana yeColitis uye 95% ~ 100% yePseudomembranous Encitis Clostrium midgele ndeye mamiriro ePathogen, kusanganisira Toxiganic starins uye isiri-toxigenic starins.
Chiteshi
| Nhamo | TcdaGene |
| Rox | tcdbGene |
| Vic / Hex | Kutonga kwemukati |
Technical parameter
| Chengetedza | ≤-18 ℃ |
| Shefu-Hupenyu | Mwedzi gumi nemaviri |
| Type Type | chitsiko |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | 200cfu / ml |
| Chokwadi | shandisa iyi kit kuti uone zvimwe zvekutambisa matanda akadai saEscherichia Aureus, Salvilla, Surmonelloly Dna, mhedzisiro yacho yese yakaipa. |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva PCR system (Hongshi Medical Technology Co, Ltd.) Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PCR Kuongororwa system (FQD-96A,HangzhouBioer tekinoroji) Ma-6000 chaiyo-nguva quolitative thermal cyler (Suzhou molarray co., Ltd.) Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Sarudzo 1.
Wedzera 180μL yeLysyme Buffer kune iyo Lysozoter (Dilute iyo Lysozyme Duliuent), pipette kuLysozyme Duliuent), pipette uye wedzera180μL yechokwadi kutonga uye kutonga kwakashata mukutevedzana. Wedzera10μl of yemukati kutonga kune iyo sampula kuti iongororwe, kutonga kwakanaka, uye kushandisa nuclec quid (YDP302) naTijin (Beijing) co. Ndokumbira utevere zvakarairwa mirairo yekushandisa kune yakatarwa nhanho. Shandisa dnase / rnase yemahara h2O kuti urege, uye iyo yakakurudzirwa elution vhoriyamu ndeye 100μl.
Sarudzo 2.
Tora 1.5ML yeRNASE / DNASE-YEMAHARA CENTRIFIGE TUBE, uye Wedzera 200μL yeKutonga Kwakanaka uye Kukanganisa Kwakashata Mukukosha. Wedzera10μl of yemukati kutonga kune iyo semuenzaniso kuti uongororwe, kutonga kwakanaka, uye kushandisa macro & micro-test-3004-32, HWTS-3004-48, HWTS-3004- 3004- 96) uye macro & micro-bvunzo otomatiki nucleic acid Excractor (HWTS-3006). Kuongororwa kunofanirwa kuitwa mukunyatsoenderana nemirairo yekushandiswa, uye iyo yakakurudzirwa vhoriyamu ndeye 80μL.
