Mycoplasma pneumoniae (mp)
Chigadzirwa Zita
HWTS-RT024 MyColasma pneumoniae (mp) nuclec acid yekuona kit (fluorescence pcr)
Epidemiology
Mycoplasma pneumoniae (MP) rudzi rwechinyararire madiki madiki, iri pakati pemabhakitiriya nehutachiona, ine madziro echitokisi asi pasina rusununguko. MP inonyanya kukonzera kutapurirana kwevanhu kutapurirana, kunyanya muvana nevechidiki. Zvinogona kukonzera kuti vanhu vanguplasma pneumonia, vana vanofema turakiti uye atypical pneumonia. Zviratidziro zvekiriniki zvakasiyana-siyana, zvakawanda zvacho zvakanyanyisa kukosora, fivha, kutonhora, kurwadziwa kwemusoro, kurwadziwa kwehuro. Pamusoro pekufema turakiti turakiti uye bronchial pneumonia inonyanya kujairika. Vamwe varwere vanogona kuvandudza kubva kune vekumusoro kufema turakiti kutapurukira kukunda zvakanyanya, kunetseka kwakanyanya uye kufa zvinogona kuitika.
Chiteshi
| Nhamo | Mycoplasma pneumoniae |
| Vic / Hex | Kutonga kwemukati |
Technical parameter
| Chengetedza | ≤-18 ℃ |
| Shefu-Hupenyu | Mwedzi gumi nemaviri |
| Type Type | Sputum, Orophaaryngeal Swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lod | 200 makopi / ml |
| Chokwadi | a) Cross Octication: Iko hakuna kuomeswa kweUreaplasma UrealticTeum, MyColasma genrovonia, Sraphilia Pneumoniae, Staphylococcus Aureus Pneumophila, pseudomson Aeruginosa, Acinetabactor Baumannii, furuwenza bhutsi, iiiii / denfaniumoumotus, dentchorory syrus uye vanhu dengic nucleic acid. b) kugona kweAnti-kupindirana: Iko kusapindira kana zvinhu zvinotevera zvakaedzwa zvichitevera: Hemoglobin (50mg / l), Bilirub / dl), mucin (60mg / ml), 10% (v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / v / Ropa revanhu, revofloxacin (10μg / ml), moxifloxacin (0.1g / l), Gemifloxacin (80μG / ML), Azithromycin (1mg / ml), clarithromycin (125μg / ml), Erythromycin ( |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva PCR system (Hongshi Medical Technology Co, Ltd.) Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PCR Kuongororwa System (FQD-96A, Hangzhou Bioer Technology) Ma-6000 chaiyo-nguva quolitative thermal cyler (Suzhou molarray co., Ltd.) Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
(1) Sputum sampuli
Yakakurudzirwa Reagent Reagent: Macro & Micro & Micro-Test Virara / rna Kit (HWTS-3017-50, HWTS-3017-98) (iyo inogona kushandiswa nemacro & micro-bvunzo Otomatiki Acleic acid Extractor (HWTS-3006C, HWTS-3006B)) NaJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Wedzera 200μl yeyakajairwa saline kune yakagadziriswa yakagadzirirwa. Inotevera chidimbu chinofanira kuitwa zvinoenderana nemirairo yekushandiswa. Iyo Yakakurudzirwa Elution Vhoriyamu inosvika 80μL.recomed Regency Regent: Nucleic acid yekubvisa kana kuchenesa Reagent (YDP315-R). Kuongororwa kunofanirwa kuitwa zvakanyatsoenderana maererano nemirairo yekushandiswa. Iyo Yakakurudzirwa Elution Vhoriyamu ndeye60μl.
(2) Orophaaryngeal Swab
Yakakurudzirwa Reagent Reagent: Macro & Micro & Micro-Test Virara / rna Kit (HWTS-3017-50, HWTS-3017-98) (iyo inogona kushandiswa nemacro & micro-bvunzo Otomatiki Acleic acid Extractor (HWTS-3006C, HWTS-3006B)) NaJiangsu Macro & Micro-Test Med-Tech Co, LTD. Kuongororwa kunofanirwa kuitwa zvinoenderana nemirairo yekushandiswa. Iyo yakakurudzirwa vhoriyamu yeSampuli is 200μL, uye iyo yakakurudzirwa elution.reamp viraral rna kit (52904) kana nucleic acid yekubvisa kana kuchenesa Reagent (YDP315-R). Kuongororwa kunofanirwa kuitwa zvakanyatsoenderana maererano nemirairo yekushandiswa. Iyo yakakurudzirwa yekubvisa vhoriyamu yemuenzaniso ndeye 140μL, uye iyo Yakakurudzirwa Vhoriyamu Bule i 60μL.
