Mycoplasma Pneumoniae (MP)
Product name
HWTS-RT024 Mycoplasma Pneumoniae (MP) Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) imhando yediki diki prokaryotic microorganism, iri pakati pebhakitiriya nehutachiona, ine sero chimiro asi isina sero madziro.MP inonyanya kukonzeresa hutachiona hwekufema kwevanhu, kunyanya muvana nevadiki.Inogona kukonzera mycoplasma pneumonia yevanhu, chirwere chevana chekufema uye atypical pneumonia.Kuratidzwa kwekiriniki kwakasiyana-siyana, mazhinji acho ari kukosora kwakanyanya, fivha, kutonhora, musoro, kurwadziwa kwehuro.Utachiona hwepamusoro hwekufema uye bronchial pneumonia ndiyo inonyanya kupararira.Vamwe varwere vanogona kukura kubva kuhutachiona hwepamusoro hwekufema kusvika kumabayo akanyanya, kushushikana kwakanyanya uye kufa kunogona kuitika.
Channel
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | Sputum, Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Makopi/mL |
| Zvakananga | a） Cross reactivity: hapana muchinjiko reactivity neUreaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacteriuinodosaculosculonese, Lefilococcus aureus, Lebakteriudoinodosa Aphilamonase bacter baumannii, Influenza A virus , Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus uye human genomic nucleic acid. b）Anti-kupindira kugona: hapana kupindira apo zvinhu zvinokanganisa zvakaedzwa nezvikamu zvinotevera: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) ropa revanhu, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg) /L), minocycline (0.1g/L). |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System (FQD-96A, Hangzhou Bioer tekinoroji) MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
(1) Sputum Muenzaniso
Inokurudzirwa kuburitsa reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Wedzera 200µL yesaline yakajairika kumvura yakagadziriswa.Kutora kunotevera kunofanirwa kuitwa maererano nekuraira kwekushandisa.Hhomuremu yakakurudzirwa ndeye 80µL. Inokurudzirwa kudhonza reagent: Nucleic Acid Extraction kana Purification Reagent (YDP315-R).Kubvisa kunofanira kuitwa zvakasimba maererano nemurairo wekushandisa.Iyo yakakurudzirwa elution vhoriyamu i60µL.
(2) Oropharyngeal swab
Inokurudzirwa kuburitsa reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubviswa kunofanira kuitwa maererano nemurairo wekushandisa.Iyo yakakurudzirwa yekubvisa vhoriyamu yemuenzaniso i200µL, uye iyo yakakurudzirwa elution vhoriyamu i80µL. Recommended extraction reagent: QIAamp Viral RNA Mini Kit (52904) kana Nucleic Acid Extraction kana Purification Reagent (YDP315-R).Kubvisa kunofanira kuitwa zvakasimba maererano nemurairo wekushandisa.Iyo yakakurudzirwa yekuwedzera vhoriyamu yemuenzaniso ndeye 140µL, uye yakakurudzirwa elution vhoriyamu i60µL.
