Mycoplasma Pneumoniae (MP)
Zita rechigadzirwa
HWTS-RT024 Mycoplasma Pneumoniae（MP） Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) rudzi rwehutachiona hudiki hweprokaryotic, huri pakati pebhakitiriya nehutachiona, hune chimiro chemasero asi husina madziro emasero. MP inonyanya kukonzera hutachiona hwekufema kwevanhu, kunyanya kuvana nevechidiki. Hunogona kukonzera mycoplasma pneumonia yevanhu, hutachiona hwekufema kwevana uye pneumonia isingawanzoitiki. Zviratidzo zvekiriniki zvakasiyana, zvakawanda zvacho kukosora kwakanyanya, fivha, dzihwa, musoro, kurwadziwa pahuro. Utachiona hwekufema hwepamusoro uye pneumonia yebronchial ndizvo zvinonyanya kuitika. Vamwe varwere vanogona kukura kubva pahutachiona hwekufema hwepamusoro kusvika papneumonia yakanyanya, kufema kwakanyanya uye rufu runogona kuitika.
Chiteshi
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Chipunu, swab yemudumbu |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi mazana maviri/mL |
| Kunyatsojeka | a） Kupindirana kwemasero: hapana kupindirana kwemasero neUreaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus uye human genomic nucleic acid. b）Kugona Kudzivisa Kukanganiswa: hapana kukanganiswa kana zvinhu zvinokanganisa zvikaedzwa nezvikamu zvinotevera: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) ropa revanhu, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
(1) Muenzaniso weMatapu
Reagent inokurudzirwa yekuburitsa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Wedzera 200µL yemunyu wakajairika kune precipitate yakagadziriswa. Kuburitsa kunotevera kunofanirwa kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu yekubvisa inokurudzirwa i80µL. Reagent inokurudzirwa yekuburitsa: Nucleic Acid Extraction kana Purification Reagent (YDP315-R). Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nemirairo yekushandisa. Vhoriyamu yekubvisa inokurudzirwa i60µL.
(2) Chinobvisa dumbu mudumbu
Reagent inokurudzirwa yekuburitsa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuburitsa kunofanirwa kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu inokurudzirwa yekuburitsa sampuro i200µL, uye vhoriyamu inokurudzirwa yekudzikisa i80µL. Reagent inokurudzirwa yekuburitsa: QIAamp Viral RNA Mini Kit (52904) kana Nucleic Acid Extraction kana Purification Reagent (YDP315-R). Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nemirairo yekushandisa. Vhoriyamu inokurudzirwa yekuburitsa sampuro i140µL, uye vhoriyamu inokurudzirwa yekuburitsa sampuro i60µL.
