Enterovirus 71 (EV71)
Product name
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hand-foot-mouth chirwere chirwere chinotapukira chinokonzerwa ne enteroviruses (EV).Parizvino, 108 marudzi e-serotypes e-enteroviruses akawanikwa, ayo akakamurwa kuva mapoka mana: A, B, C uye D. Pakati pavo, enterovirus EV71 uye CoxA16 ndiyo huru yezvirwere.Chirwere ichi chinowanzoitika muvana vari pasi pemakore mashanu, uye chinogona kukonzera herpes pamaoko, tsoka, muromo uye zvimwe zvikamu.Nhamba shoma yevana ichaita matambudziko akadai semyocarditis, pulmonary edema, uye aseptic meningoencephalitis.
Channel
| FAM | EV71 |
| ROX | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 9 mwedzi |
| Type Type | Oropharyngeal swab,Herpes fluid |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Makopi/mL |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR Systems LineGene 9600 Plus Chaiyo-Nguva PCR Detection Systems MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
Inokurudzirwa kudhirowa reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), uye kudhirowa kunofanirwa kuitwa zvinoenderana nekuraira kwekushandisa.Iwo akabviswa samples ndeye oropharyngeal swabs kana herpes fluid samples kubva kuvarwere vakaunganidzwa panzvimbo.Wedzera iyo yakaunganidzwa swabs muMacro & Micro-Test Sample Release Reagent (HWTS-3005-8) zvakananga, vortex uye sanganisa zvakanaka, isa pamhepo tembiricha kwemaminetsi mashanu, ibudise kunze uye wozopinza kuti usanganise zvakanaka, kuti uwane iyo RNA. yemuenzaniso wega wega.
Sarudzo 2.
Inokurudzirwa kuburitsa reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd., uye kudhirowa kunofanirwa kuitwa zvinoenderana nekuraira kwekushandisa.Iyo yakatorwa vhoriyamu yemuenzaniso ndeye 200µL, uye yakakurudzirwa elution vhoriyamu i80µL.
Sarudzo3.
Recommended extraction reagent: QIAamp Viral RNA Mini Kit (52904) by QIAGEN kana TIANamp Virus DNA/RNA Kit (YDP315-R), uye kuchera kunofanira kuitwa zvichinyatsoenderana nerairo yekushandisa.Iyo yakatorwa sample vhoriyamu i140μL, uye yakakurudzirwa elution vhoriyamu i60µL.
