Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin (RIF)，Resistance (INH)

Mufananidzo Unoratidza Mycobacterium Tuberculosis Nucleic Acid neRifampicin(RIF)，Resistance(INH)

Tsananguro pfupi:

Kit iyi inoshandiswa pakuongorora DNA yeMycobacterium tuberculosis muhuman sputum, solid culture (LJ Medium) uye liquid culture (MGIT Medium), bronchial lavage fluid, uye mutations mu 507-533 amino acid codon region (81bp, rifampicin resistance determining region) ye rpoB gene yeMycobacterium tuberculosis rifampicin resistance, pamwe ne mutations munzvimbo huru dzemutation yeMycobacterium tuberculosis isoniazid resistance. Inobatsira pakuongorora hutachiona hweMycobacterium tuberculosis, uye inoona majini makuru emutambi werifampicin neisoniazid, izvo zvinobatsira kunzwisisa mushonga weMycobacterium tuberculosis inotapukirwa nemurwere.

Tumira email kwatiri

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Zita rechigadzirwa

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid neRifampicin(RIF), (INH) Detection Kit (Melting Curve)

Epidemiology

Mycobacterium tuberculosis, inozivikanwa seTubercle bacillus (TB), ndiyo bhakitiriya inokonzera tuberculosis, uye pari zvino, mishonga yekutanga inoshandiswa pakurwisa tuberculosis inosanganisira isoniazid, rifampicin neethambutol, nezvimwewo.^[1]Zvisinei, nekuda kwekushandiswa zvisirizvo kwemishonga yekurwisa TB uye hunhu hwemadziro emasero e mycobacterium tuberculosis pachayo, mycobacterium tuberculosis yakava nekuramba kwemishonga yekurwisa TB, uye mhando ine njodzi zvikuru i multidrug-resistant tuberculosis (MDR-TB), iyo inoramba kumishonga miviri inonyanya kushandiswa uye inoshanda, rifampicin ne isoniazid.^[2].

Dambudziko rekusagona kurapwa nemishonga yeTB riripo munyika dzese dzakaongororwa neWHO. Kuti pave nehurongwa hwakarurama hwekurapa varwere veTB, zvakakosha kuona kusagona kurapwa nemishonga yeTB, kunyanya kusagona kurapwa nerifampicin, iyo yave danho rekuongorora rinokurudzirwa neWHO mukurapa chirwere cheTB.^[3]Kunyangwe kuwanikwa kwekusagona kurwisa rifampicin kwakafanana nekuwanikwa kweMDR-TB, kuona chete kusagona kurwisa rifampicin kunoregeredza varwere vane mono-resistant INH (kureva kusagona kurwisa isoniazid asi inonzwa rifampicin) uye mono-resistant rifampicin (kunzwa kusingagone kurwisa isoniazid asi inoramba rifampicin), izvo zvinogona kutungamira kuti varwere vapihwe mishonga yekutanga isina musoro. Nokudaro, kuongororwa kwekudzivirira isoniazid nerifampicin ndizvo zvinodiwa zvishoma muzvirongwa zvese zvekudzora DR-TB.^[⁴^].

Magadzirirwo ehunyanzvi

Kuchengetera	≤-18℃
Nguva yekuzorora	mwedzi gumi nemiviri
Rudzi rwemuenzaniso	Muenzaniso weSputum, Kukura Kwakasimba (LJ Medium), Kukura Kwemvura (MGIT Medium)
CV	<5.0%
LoD	LoD yekit yekuona Mycobacterium tuberculosis i10 bacteria/mL;LoD yekit yekuona rifampicin wild type uye mutant type i150 bacteria/mL; LoD yekit yekuona rudzi rwemusango rweisoniazid nerudzi rwemutant i200 bacteria/mL.
Kunyatsojeka	1) Hapana kusangana kunoitika kana uchishandisa kit iyi kuona DNA yemunhu (500ng), mamwe marudzi makumi maviri nemasere ezvirwere zvekufema, uye marudzi makumi maviri nemapfumbamwe e mycobacteria isiri TB (sezvinoratidzwa muTafura 3).2) Hapana mhinduro inosanganisirwa pakushandisa kit iyi kuona nzvimbo dzekuchinja kwemamwe majini erifampicin uye Mycobacterium tuberculosis asingagoni kurapwa nemishonga (sezvakaratidzwa muTafura 4).3) Zvinhu zvinokanganisa masero zviri mumienzaniso inofanira kuyedzwa, zvakaita serifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L) mishonga, hazvina zvazvinoita pamhedzisiro yeongororo yekit.
Zvishandiso Zvinoshanda	Masisitimu ePCR eSLAN-96P eNguva Yechokwadi (Hongshi Medical Technology Co., Ltd.), Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi