Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin(RIF)，Resistance(INH)

Tsanangudzo Pfupi

Kiti iyi inoshandiswa pakuongorora in vitro qualitative yeMycobacterium tuberculosis DNA musputum yemunhu, yakasimba tsika (LJ Medium) uye fluid tsika (MGIT Medium), bronchial lavage fluid, uye shanduko munharaunda ye507-533 amino acid codon (81bp, rifampicin kuramba inotaridza nharaunda yerifampicin tuberculosis dunhu reMbacteriofampicin). pamwe chete neshanduko munzvimbo huru dzeMycobacterium tuberculosis isoniazid resistance.Inopa rubatsiro pakuongororwa kwechirwere cheMycobacterium tuberculosis, uye inoona magenenes makuru ekurwisa rifampicin neisoniazid, ayo anobatsira kunzwisisa kusamira kwemishonga kweMycobacterium tuberculosis inobatwa nemurwere.

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Product Detail

Product Tags

Product name

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin(RIF), (INH) Detection Kit (Melting Curve)

Epidemiology

Mycobacterium tuberculosis, munguva pfupi yainzi Tubercle bacillus (TB), utachiona hwepathogenic hunokonzera chirwere cherurindi, uye pari zvino, mishonga yemhando yekutanga inorwisa tibhii inowanzoshandiswa inosanganisira isoniazid, rifampicin ne ethambutol, nezvimwewo.^[1]. Nekudaro, nekuda kwekusashandiswa zvisirizvo kwemishonga inorwisa tuberculosis uye hunhu hwesero madziro chimiro che mycobacterium tuberculosis pachayo, mycobacterium tuberculosis yakagadzira kurwisa kwemishonga inorwisa-tibhii, uye fomu ine njodzi yakanyanya ndeye multidrug-resistant tuberculosis (MDR-TB), iyo inopokana nemishonga miviri inonyanyozivikanwa uye inoshanda, rifampicin uye irifampicin.^[2].

Dambudziko rechirwere cherurindi rekusabatwa nemishonga ririko munyika dzose dzakaongororwa neWHO. Kuti tipe zvirongwa zvekurapa zvine huremu hwevarwere verurindi, zvakakosha kuti paonekwe kuti haamiri kumishonga inorwisa TB, kunyanya rifampicin, iro rave danho rekuongorora rinokurudzirwa neWHO mukurapa tibhii.^[3]. Kunyange zvazvo kuwanikwa kwerifampicin resistance kunenge kwakada kuenzana nekuwanikwa kweMDR-TB, kungoona kuti rifampicin haina kumira zvakanaka kunofuratira varwere vane mono-resistant INH (zvichireva resistance to isoniazid asi inonzwa rifampicin) uye mono-resistant rifampicin (sensitivity to isoniazid but resistance to rifampicin), izvo zvinogona kuita kuti varwere vatadze kurapwa. Naizvozvo, bvunzo dzekupikiswa kweisoniazid nerifampicin zvidiki zvinodiwa muzvirongwa zvese zvekudzora DR-TB^[⁴^].

Technical Parameters

Storage	≤-18℃
Sherufu-hupenyu	12 mwedzi
Type Type	Sputum Sample, Solid Tsika (LJ Medium), Liquid Tsika (MGIT Medium)
CV	<5.0%
LoD	Iyo LoD yekiti yekuona Mycobacterium tuberculosis ndeye 10 mabhakitiriya / mL;iyo LoD yekiti yekuona rifampicin mhando yemusango uye mutant mhando i150 mabhakitiriya/mL; iyo LoD yekiti yekuona isoniazid yemusango mhando uye mutant mhando i200 bacteria/mL.
Zvakananga	1) Hapana kupesana kwepamuchinjikwa paunenge uchishandisa kititi kuti uone genomic DNA yevanhu (500ng), mamwe marudzi e28 ezvirwere zvekufema, uye 29 marudzi e-non-tuberculous mycobacteria (sezvinoratidzwa muTebhu 3).2) Hapana kupindirana kana uchishandisa kiti kuona nzvimbo dzekuchinja dzemamwe majini asingaurayiwi nemishonga erifampicin uye isoniazid inoziva Mycobacterium tuberculosis (sezvinoratidzwa muTebhurari 4).3) Zvinowanzo vhiringa zvinhu mumasampuli zvinoongororwa, se rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanaxamisomg/L (20mg/L) zvinodhaka, hazvina zvazvinoita pamhedzisiro yebvunzo dzekiti.
Zvishandiso Zvishandiso	SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Chaiyo-Nguva PCR System