Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin(RIF)，Isoniazid Resistance(INH)
Product name
HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, kwenguva pfupi seTubercle bacillus (TB), utachiona hwepathogenic hunokonzera tibhii.Parizvino, mishonga inowanzoshandiswa yemutsara wekutanga inorwisa tuberculosis inosanganisira isoniazid, rifampicin uye ethambutol, nezvimwewo. Mishonga yepiriviri inorwisa tuberculosis inosanganisira fluoroquinolones, amikacin uye kanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa inonzi linezolid, bedaquiline nedelamani, nezvimwewo. .Zvisinei, nekuda kwekusashandiswa zvisirizvo kwemishonga inorwisa tibhii uye hunhu hwesero rusvingo rwemycobacterium tuberculosis, mycobacterium tuberculosis inokura kurwisa zvinodhaka kumishonga inorwisa tibhii, izvo zvinounza matambudziko akakomba mukudzivirira uye kurapwa kwetibhii.
Channel
| Zita rechinangwa | Reporter | Quencher | ||
| Reaction BufferA | Reaction BufferB | Reaction BufferC | ||
| rpoB 507-514 | rpoB 513-520 | IS6110 | FAM | Hapana |
| rpoB 520-527 | rpoB 527-533 | / | CY5 | Hapana |
| / | / | Kudzora mukati | HEX(VIC) | Hapana |
| Reaction BufferD | Reporter | Quencher |
| InhA promotioner region -15C>T, -8T>A, -8T>C | FAM | Hapana |
| KatG 315 kodon 315G>A,315G>C | CY5 | Hapana |
| AhpC inosimudzira dunhu -12C>T, -6G>A | ROX | Hapana |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | sputum |
| CV | ≤5.0% |
| LoD | Iyo LoD ye mycobacterium tuberculosis yenyika inoreva mabhakitiriya makumi mashanu pamL.Iyo LoD ye rifampicin-resistant mhando yenyika referensi ndeye 2×103mabhakitiriya/mL, uye iyo LoD yemhando inoshanduka ndeye 2 × 103utachiona/mL.Iyo LoD yemusango-mhando isoniazid inodzivirira bhakitiriya ndeye 2x103bhakitiriya/mL, uye Iyo LoD yemabhakitiriya anoshanduka ndeye 2x103utachiona/mL. |
| Zvakananga | Mhedzisiro yebvunzo yemhedzisiro yakaratidza kuti pakanga pasina kuita kwemuchinjikwa mukuonekwa kwejenome remunhu, mamwe asiri tuberculosis mycobacteria nemabayo pathogens nekiti iyi;Pakange pasina kuchinjika kwakaonekwa panzvimbo dzekuchinja kwemamwe majini anoramba zvinodhaka mune yemusango-mhando Mycobacterium tuberculosis. |
| Zvishandiso Zvishandiso | SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Chaiyo-Nguva PCR System, Hangzhou Bioer tekinoroji QuantGene 9600 Chaiyo-Nguva PCR System, Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR System.
|
Total PCR Solution
