Virusi yeMonkeypox uye Typing Nucleic Acid

Mufananidzo Unoratidza Virusi reMonkeypox neTyping Nucleic Acid

Tsananguro pfupi:

Kit iyi inoshandiswa pakuona ma "monkeypox virus clade I, clade II" uye "monkeypox virus universal nucleic acids" mumvura ye "human rash fluid", "oropharyngeal swabs" uye "serum samples" mu "in vitro".

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Kiti yeHWTS-OT202-Monkeypox Virus uye Typing Nucleic Acid Detection Kit (Fluorescence PCR)

Chitupa

Epidemiology

Monkeypox (Mpox) chirwere chinotapukira chinokonzerwa neMonkeypox Virus (MPXV). MPXV ine chimiro chedenderedzwa kana kuti denderedzwa, uye ivhairasi yeDNA ine tambo mbiri ine hurefu hweinenge 197Kb.^[1]Chirwere ichi chinonyanya kutapuriranwa nemhuka, uye vanhu vanogona kutapukirwa nekurumwa nemhuka dzine chirwere ichi kana nekubata zvakananga ropa, mvura yemuviri uye mapundu emhuka dzine chirwere ichi. Hutachiona hunogonawo kutapurirwa pakati pevanhu, kunyanya kuburikidza nemadonhwe ekufema panguva yekusangana zvakananga kana kuti nekubata zvakananga nemvura yemuviri yemurwere kana zvinhu zvakasvibiswa.^[2-3]Zvidzidzo zvakaratidza kuti MPXV inoumba zvidimbu zviviri zvakasiyana: clade I (yaimbozivikanwa seCentral African clade kana kuti Congo Basin clade) uye clade II (yaimbonzi West African clade). Mpox yeCongo Basin clade yakaratidzwa zvakajeka kuti inotapurirana pakati pevanhu uye inogona kukonzera rufu, nepo mpox yeWest African clade ichikonzera zviratidzo zvishoma uye ine mwero wakaderera wekutapurirana kubva kuvanhu kuenda kuvanhu.^[4].

Mhedzisiro yebvunzo iyi haisi iyo chete inoratidza kuongororwa kwehutachiona hweMPXV muvarwere, iyo inofanirwa kubatanidzwa nehunhu hwemurwere pamwe nedzimwe data rebvunzo yerabhoritari kuti vaone nemazvo hutachiona hwehutachiona uye kugadzira hurongwa hwekurapa hwakakodzera kuti vave nechokwadi chekuti kurapwa kwakachengeteka uye kunoshanda.

Chiteshi

FAM	MPXV chikamu chechipiri
ROX	MPXV universal nucleic acid
VIC/HEX	MPXV chikamu I
CY5	kudzora kwemukati

Magadzirirwo ehunyanzvi

Kuchengetera	≤-18℃
Nguva yekuzorora	mwedzi gumi nemiviri
Rudzi rwemuenzaniso	mvura yeganda revanhu, machubhu emuromo uye seramu
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35（IC)
LoD	Makopi mazana maviri/mL
Zvishandiso Zvinoshanda	Chinogadzirisa kuona mhando yekutanga: Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio^®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Chiedza cheKufambisa Mwenje^®Sisitimu yePCR ye480 Real-Time Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi Chinogadzirisa kuonekwa kwerudzi rwechipiri: Eudemon^TMAIO800 (HWTS-EQ007)) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.