Klebsiella Pneumoniae, Acinetobacter Baumannii uye Pseudomonas Aeruginosa uye Drug Resistance Genes (KPC, NDM, OXA48 uye IMP) Multiplex
Zita rechigadzirwa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii uye Pseudomonas Aeruginosa uye Drug Resistance Genes (KPC, NDM, OXA48 uye IMP) Multiplex Detection Kit (Fluorescence PCR)
Chitupa
CE
Epidemiology
Klebsiella pneumoniae chirwere chinowanzo wanikwa muchipatara uye chimwe chezvirwere zvikuru zvinokonzera hutachiona hwepasocomial. Kana simba remuviri radzikira, mabhakitiriya anopinda mumapapu achibva mudumbu, zvichikonzera hutachiona muzvikamu zvakawanda zvemuviri, uye kushandiswa kwekutanga kwemishonga inorwisa mabhakitiriya ndiyo kiyi yekurapa [1]. Nzvimbo inonyanya kuwanikwa hutachiona hweAcinetobacter baumannii imapapu, inova chirwere chakakosha cheHospital acquired pneumonia (HAP), kunyanya Ventilator associated pneumonia (VAP). Inowanzo perekedzwa nedzimwe hutachiona hwemabhakitiriya nefungus, hune hunhu hwehuwandu hwezvirwere uye huwandu hwerufu.Pseudomonas aeruginosa ndiyo bacilli isingabereki gram-negative inonyanya kuwanikwa mukiriniki, uye chirwere chakakosha chehutachiona hunowanikwa muchipatara, hune hunhu hwekupinda muropa nyore, kusiyana kuri nyore uye kuramba mishonga yakawanda.
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Masvoto |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | Makopi 1000/mL |
| Kunyatsojeka | a)Kuongororwa kwekuongorora kwehutachiona kunoratidza kuti kit iyi haina hutachiona hwehutachiona hwehutachiona hwekufema hwakafanana nedzimwe hutachiona hwekufema, dzakadai seStreptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus uye sputum samples dzisina zvinangwa, nezvimwewo. b)Kugona Kudzivisa Kukanganiswa: Sarudza mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, uye roxithromycin, nezvimwewo. kuti uedze kukanganiswa, uye mhedzisiro inoratidza kuti zvinhu zvinokanganisa zvataurwa pamusoro hazvikanganisi kuonekwa kweKlebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa uye carbapenem resistance genes KPC, NDM, OXA48 uye IMP. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu 7500 Masisitimu EPCR Enguva Yechokwadi, Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Nguva Yechokwadi, QuantStudio®Masisitimu mashanu ePCR eNguva Yechokwadi, Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time, Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeBioer), MA-6000 Chaiyo-Nguva Yese Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.), Sisitimu yePCR yeBioRad CFX96 Real-Time, Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi. |
Kufamba Kwebasa
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. inokurudzirwa pakutorwa kwemuenzaniso uye matanho anotevera anofanira kuitwa zvichienderana neIFU yeKit.
