Kuchinja kweGene reEGFR reMunhu 29

Mufananidzo weHuman EGFR Gene 29 Mutations

Tsananguro pfupi:

Kit iyi inoshandiswa kuona shanduko dzakajairika mu exons 18-21 yejini reEGFR muzviyero kubva kuvarwere vekenza yemapapu vasiri masero madiki.

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Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Zita rechigadzirwa

HWTS-TM0012A-Human EGFR Gene 29 Mutations Detection Kit (Fluorescence PCR)

Epidemiology

Kenza yemapapu yave chinhu chikuru chinokonzera kufa kwekenza pasi rose, zvichikonzera njodzi huru kuhutano hwevanhu. Kenza yemapapu isiri diki inoumba vanhu vanosvika 80% yevarwere vane kenza yemapapu. EGFR ndiyo inonyanya kukosha pakurapa kenza yemapapu isiri diki. Kuumbwa kwe phosphorylation ye EGFR kunogona kukurudzira kukura kwemasero ekenza, kusiyanisa, kupinda mumasero, kupararira kwemasero, kurwisa apoptosis, uye kukurudzira angiogenesis yekenza. EGFR tyrosine kinase inhibitors (TKI) inogona kuvharira nzira yechiratidzo che EGFR nekudzivisa EGFR autophosphorylation, nokudaro ichidzivisa kuwanda nekusiyana kwemasero ekenza, kukurudzira apoptosis yemasero ekenza, kuderedza angiogenesis yekenza, nezvimwewo, kuitira kuti iwane kurapwa kwakanangana nekenza. Ongororo dzakawanda dzakaratidza kuti kushanda kweEGFR-TKI pakurapa kwakabatana zvikuru nemamiriro ekuchinja kwemajini eEGFR, uye kunogona kudzivirira kukura kwemasero ekenza nekuchinja kwemajini eEGFR. Jini reEGFR riri paruoko rupfupi rwechromosome 7 (7p12), rune hurefu hwakazara hwe200Kb uye rine maexons 28. Nzvimbo yakachinja inowanikwa zvakanyanya muexons 18 kusvika 21, codons 746 kusvika 753 deletion mutation pa exon 19 inokwana 45% uye L858R mutation pa exon 21 inokwana 40% kusvika 45%. NCCN Guidelines for the Diagnosis and Treatment of Non-Small Cell Lung Cancer inotaura zvakajeka kuti bvunzo dzeEGFR gene mutation dzinodiwa EGFR-TKI isati yapihwa. Iyi test kit inoshandiswa kutungamira kupihwa kwemishonga ye epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), uye inopa hwaro hwemishonga yakagadzirirwa varwere vane kenza yemapapu isiri diki. Iyi kit inoshandiswa chete pakuona shanduko dzakajairika mujini reEGFR muvarwere vane kenza yemapapu isiri diki. Mhedzisiro yebvunzo ndeyekutarisa kukiriniki chete uye haifanirwe kushandiswa senzira chete yekurapa varwere vakasiyana. Vanachiremba vanofanirwa kufunga nezvemamiriro emurwere, zviratidzo zvemushonga, uye kurapwa. Maitiro ake nezvimwe zviratidzo zvebvunzo yerabhoritari nezvimwe zvinhu zvinoshandiswa kutonga zvakakwana mhedzisiro yebvunzo.

Chiteshi

FAM	IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer

Magadzirirwo ehunyanzvi

Kuchengetera	Mvura: ≤-18℃ Murima; Yakapfava: ≤30℃ Murima
Nguva yekuzorora	Mvura: mwedzi 9; Lyophilized: mwedzi 12
Rudzi rwemuenzaniso	nyama itsva yebundu, chikamu chechirwere chakaomeswa nechando, nyama kana chikamu chechirwere chakabatanidzwa neparafini, plasma kana serum
CV	＜5.0%
LoD	Kuonekwa kwemhinduro ye nucleic acid reaction pasi pe 3ng/μL wild-type, kunogona kuona 1% mutation rate zvakanaka.
Kunyatsojeka	Hapana kusangana kweDNA ye genomic yemunhu yemhando yemusango nedzimwe mhando dzemutant.
Zvishandiso Zvinoshanda	Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva YechokwadiMaitiro Ekushandisa Ezvinhu Zvehupenyu 7300 Masisitimu EPCR Enguva Yechokwadi Masisitimu ePCR eQuantStudio® 5 Enguva Yechokwadi Sisitimu yePCR yeLightCycler® 480 Real-Time Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi