Human Brf Gene Gene v600e Mutation
Chigadzirwa Zita
HWTS-TM007-Human Brach Gene V600e Mutation Kuona Kit (Fluorescence PCR)
Chitupa
CE / TFDA
Epidemiology
Mhando dzinopfuura makumi matatu dzeBraf Muchetati dzeBraati dzakawanikwa, dzipi zvaro dzinosvika 90% muchishandiso Adenine (a), zvichikonzera kutsiva kweValine (v) panzvimbo 600 ne glutamic acid (e) muProtein chigadzirwa. Braf muchitiki anowanzo kuwanikwa mumatauriri akaipa senge melanoma, colerol cancer, cancer yegomarara, uye kenza yemapapu. Kunzwisisa mutaundi reBraf Gene rave kudiwa kwekuchera egfr-tkis genge gene mutation-rakarongedzwa zvinodhaka zvakarongeka kurapwa kwevarwere vanogona kubatsirwa.
Chiteshi
Nhamo | V600e shandation, zvemukati kutonga |
Technical parameter
Chengetedza | ≤-18 ℃ |
Shefu-Hupenyu | 9 mwedzi |
Type Type | paraffin-yakamiswa pathological tishu samples |
CV | <5.0% |
Ct | ≤38 |
Lod | Shandisa kizi kuti uone inoenderana inoenderana nemhando yemhando. a) Under 3ng / μl Wild-Type Background, 1% Mutaundi chiyero chinogona kuonekwa mune reaction buffer zvinoshamisa; b) pasi pe1% mutation chiyero, iyo mutation ye1 × 103Makopi / ml mune yemusango-mhando kumashure kwe1 × 105Makopi / mL inogona kuonwa zvine hunyanzvi mune reaction buffer; c) Iyo IC reaction buffer inogona kuona yakaderera yekuona chikamu chekudzora mhando sw3 yekambani yemukati control. |
Zviridzwa Zvinoshanda: | Yakaiswa Biosystems 7500 chaiyo-nguva PCR systemYakaisa biosystems 7300 chaiyo-nguva pcr Systems, Quantstudio® 5 chaiyo-nguva PCR system Lightcycler ®480 chaiyo-nguva PCR system Biorad cfx96 chaiyo-nguva pcr system |
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