Kuchinja kweGene reBRAF reMunhu V600E
Zita rechigadzirwa
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit (Fluorescence PCR)
Chitupa
CE/TFDA
Epidemiology
Mhando dzinopfuura makumi matatu dzekuchinja kweBRAF dzakawanikwa, idzo dzinenge 90% dziri mu exon 15, uko kuchinja kweV600E kunoonekwa sekuchinja kwakajairika, ndiko kuti, thymine(T) iri panzvimbo ye1799 mu exon 15 inochinjwa kuita adenine (A), zvichikonzera kutsiviwa kwevaline (V) iri panzvimbo ye600 neglutamic acid (E) muprotein product. Kuchinja kweBRAF kunowanzo kuwanikwa mumabundu ane njodzi akadai se melanoma, kenza ye colorectal, kenza ye thyroid, uye kenza yemapapu. Kunzwisisa kuchinja kweBRAF gene kwave kudikanwa kwekuongorora EGFR-TKIs neBRAF gene mutation-targeted drugs mu clinical target drug therapy kune varwere vanogona kubatsirwa.
Chiteshi
| FAM | Kuchinja kweV600E, kutonga kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | mienzaniso yezvirwere zvemuviri zvakaiswa muparafini |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Shandisa makits kuti uone kudzora kwemhando yeLoD kunoenderana. a) Pasi pe3ng/μL wild-type background, 1% mutation rate inogona kuonekwa mu reaction buffer zvakanaka; b) pasi pe1% mutation rate, mutation ye1×103Makopi/mL ari muchimiro chemusango che1×105Makopi/mL anogona kuonekwa zvakanaka mu reaction buffer; c) IC Reaction Buffer inogona kuona muganho wepasi wekuona muganho wemhando yeSW3 wekutonga kwemukati mekambani. |
| Zvishandiso Zvinoshanda: | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva YechokwadiApplied Biosystems 7300 Real-Time PCR Masisitimu, QuantStudio® 5 Masisitimu ePCR Enguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi |
Kufamba Kwebasa
Zvinokurudzirwa kuti zvibviswe: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yakagadzirwa neTiangen Biotech(Beijing) Co., Ltd.
