Yakaomeswa nechando Zaire neSudan Ebolavirus Nucleic Acid
Product name
HWTS-FE035-Freeze-dried Zaire neSudan Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ebolavirus ndeye Filoviridae, iyo isina kupatsanurwa imwe-stranded negative-strand RNA virus. Utachiona hurefu hurefu hurefu hwevirion hunosvika 1000nm uye dhayamita ingangoita 100nm. Ebolavirus genome is an unsegmented negative-strand RNA ine saizi ye18.9kb, encoding 7 structural proteins uye 1 non-structural protein. Ebolavirus inogona kukamurwa kuita mhando seZaire, Sudan, Bundibugyo, Tai Forest uye Reston. Pakati pavo, mhando yeZaire neSudan zvinonzi zvakakonzera kufa kwevanhu vakawanda neutachiona. EHF (Ebola Hemorrhagic Fever) chirwere chinotapukira chinotapukira chinokonzerwa neEbolavirus. Vanhu vanonyanya kutapukirwa nekubatana nemvura yemumuviri, kuvharwa uye tsvina yevarwere kana mhuka dzine utachiona, uye zviratidzo zvekiriniki zvinonyanya kubuda kupisa, kubuda ropa uye kukanganisa kwenhengo dzakawanda. EHF ine mwero wepamusoro wekufa kwe50% -90%. Parizvino, nzira dzekuongororwa kweEbolavirus dzinonyanya kuongororwa murabhoritari, kusanganisira zvinhu zviviri: etiological yekuona uye serological yekuona. Etiological diagnosis inosanganisira kuona hutachiona hwemaantigens mumasampuli eropa neELISA, kuona nucleic acids nenzira dzekusimudzira dzakadai seRT-PCR, nezvimwewo, uye kushandisa masero Vero, Hela, nezvimwewo pakuparadzaniswa kwehutachiona uye tsika. Kuonekwa kweSerological kunosanganisira kuona Serum chaiyo IgM masoja ekudzivirira chirwere nekutora ELISA, uye kuona Serum chaiyo IgG masoja ekudzivirira chirwere neELISA, immunofluorescence, nezvimwe.
Technical Parameters
| Storage | ≤30℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | serum, plasma samples |
| CV | ≤5.0% |
| LoD | 500 Makopi/μL |
| Zvishandiso Zvishandiso | Inoshanda kutaipa I yekuona reagent: Applied Biosystems 7500 Chaiyo-Nguva PCR Systems, Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems, SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Chaiyo-Nguva PCR Detection Systems (FQD-96A, Hangzhou Bioer tekinoroji), MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Chaiyo-Nguva PCR System, BioRad CFX Opus 96 Chaiyo-Nguva PCR System. Inoshanda kune Type II yekuona reagent: EudemonTMAIO800 (HWTS-EQ007) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kuyerera Kwebasa
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Iko kudhirowa kunofanirwa kuitwa zvinoenderana nemirairo, uye yakatorwa sampuro vhoriyamu i200μL uye yakakurudzirwa elution vhoriyamu i80μL.
