Zaire yakaomeswa nechando neSudan Ebolavirus Nucleic Acid
Zita rechigadzirwa
HWTS-FE035-Kit yekuona Nucleic Acid yeZaire neSudan yakaomeswa nechando (Fluorescence PCR)
Epidemiology
Ebolavirus ndeyeFiloviridae, inova hutachiona hweRNA husina tambo imwe chete husina tambo imwe chete. Mavhairasi imhando refu ine avhareji yehurefu hwevirion hwe1000nm uye dhayamita inosvika 100nm. Genome reEbolavirus iRNA isina tambo isina tambo ine saizi ye18.9kb, ine mapuroteni manomwe emuviri uye puroteni imwe chete isina structural. Ebolavirus inogona kukamurwa kuita mhando dzakadai seZaire, Sudan, Bundibugyo, Tai Forest neReston. Pakati padzo, mhando yeZaire nerudzi rweSudan zvinonzi ndizvo zvinokonzera kufa kwevanhu vazhinji nekuda kwehutachiona. EHF (Ebola Hemorrhagic Fever) chirwere chinotapukira chinobuda ropa chinokonzerwa neEbolavirus. Vanhu vanonyanya kutapukirwa nekusangana nemvura yemuviri, tsvina uye tsvina yevarwere kana mhuka dzine hutachiona, uye zviratidzo zvekiriniki zvinonyanya kubuda fivha, kubuda ropa uye kukuvara kwenhengo dzakawanda. EHF ine huwandu hwakawanda hwerufu hwe50%-90%. Parizvino, nzira dzekuongorora Ebolavirus dzinosanganisira bvunzo dzemurabhoritari, dzinosanganisira zvinhu zviviri: kuona etiological uye kuona serological. Kuona etiological kunosanganisira kuona maantigen ehutachiona muropa kuburikidza neELISA, kuona nucleic acids nenzira dzekuwedzera seRT-PCR, nezvimwewo, uye kushandisa masero Vero, Hela, nezvimwewo kuti hutachiona husaparadzwe uye hukure. Kuona serological kunosanganisira kuona maSerum specific IgM antibodies nekubata ELISA, uye kuona maSerum specific IgG antibodies neELISA, immunofluorescence, nezvimwewo.
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤30℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | seramu, plamienzaniso yesma |
| CV | ≤5.0% |
| LoD | Makopi 500/μL |
| Zvishandiso Zvinoshanda | Inoshanda kune reagent yekuona type I: Maitiro Ekushandisa Ezvinhu 7500 Masisitimu EPCR Enguva Yechokwadi, QuantStudio®Masisitimu mashanu ePCR eNguva Yechokwadi, Masisitimu ePCR eSLAN-96P eNguva Yechokwadi (Hongshi Medical Technology Co., Ltd.), Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer), MA-6000 Chaiyo-Nguva Yese Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.), Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi, Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi. Inoshanda kune reagent yekuona mhando yechipiri: EudemonTMAIO800 (HWTS-EQ007) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kufamba Kwebasa
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kubviswa kwesampuro kunofanirwa kuitwa zvichienderana nemirairo, uye huwandu hwesampuro hwakatorwa i200μL uye huwandu hweelution hunokurudzirwa i80μL.
