Enterovirus Yese
Zita rechigadzirwa
HWTS-EV001- Kit yeKuona Acid yeEnterovirus Universal Nucleic Acid (Fluorescence PCR)
Epidemiology
Chirwere chemuromo weruoko chirwere chinotapukira chinokonzerwa nema enteroviruses (EV). Parizvino, marudzi 108 e enteroviruses akawanikwa, ayo akakamurwa kuita mapoka mana: A, B, C naD. Pakati pawo, enterovirus EV71 naCoxA16 ndiwo hutachiona hukuru. Chirwere ichi chinowanzoitika kuvana vari pasi pemakore mashanu, uye chinogona kukonzera herpes pamaoko, tsoka, muromo nedzimwe nzvimbo. Vana vashoma vanozosangana nematambudziko akadai se myocarditis, pulmonary edema, uye aseptic meningoencephalitis.
Chiteshi
| FAM | EV RNA |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Chinobvisa muromo,Mvura yeHerpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/mL |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu 7500/7500 Masisitimu EPCR Anokurumidza Munguva Yechokwadi, QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Kiti Yekuburitsa Inokurudzirwa: Kiti yeMacro & Micro-Test General DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), inofanira kutorwa zvakanyatsoenderana nemirairo. Vhoriyamu yemuenzaniso i200 μL, vhoriyamu yekudzikisa inokurudzirwa i80µL.
Sarudzo yechipiri.
Kiti inokurudzirwa yekuburitsa: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), inofanira kubudiswa zvakanyatsoenderana nemirayiridzo.
Sarudzo 3.
Kiti yekubvisa inokurudzirwa: QIAamp Viral RNA Mini Kit (52904) kana Nucleic Acid Extraction kana Purification Kit (YDP315-R), inofanira kubviswa zvichienderana nemirairo. Vhoriyamu yemuenzaniso i140 μL, vhoriyamu yekudzikisa inokurudzirwa i60µL.
