Enterovirus Universal
Chigadzirwa Zita
HWTS-EV001- Entererovirus Universal Nucleic acid Kuona Kit (Fluorescence PCR)
Epidemiology
Hosha-tsoka-yemuromo chirwere chirwere chinotapukira chinokonzerwa negumi entroviruse (Ev). Parizvino, marudzi 108 eSerotypes emakore emakore akawanikwa, ayo akakamurwa mumapoka mana: A, b, c naD. Entertrovus ev71 uye Coxa16 ndiwo mainter. Chirwere ichi chinonyanya kuitika muvana vari pasi pemakore mashanu, uye zvinogona kukonzera herpes pamaoko, tsoka, muromo uye zvimwe mumigwagwa. Nhamba diki yevana inokudziridza matambudziko akadai saMyOcararditis, Pulmonary Edema, uye Aseptic Miningoentolitis.
Chiteshi
| Nhamo | Ev rna |
| Rox | Kutonga kwemukati |
Technical parameter
| Chengetedza | ≤-18 ℃ |
| Shefu-Hupenyu | Mwedzi gumi nemaviri |
| Type Type | Orophayengeal Swab,Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lod | 500copiji / ml |
| Zviridzwa zvinoshanda | Yakaiswa biosystems 7500/7500 nekukurumidza chaiyo-nguva PCR system, Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva pcr system Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRR Kuongororwa system Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Sarudzo 1.
Rumbidzai Kititi yekubvisa: Macro & Micro & Micro-Test General DNA / RWTS HWTS-3006B, HWTS-3006C), inofanira kubviswa zvakanyanyisa maererano nemirairo. Iyo sampuli vhoriyamu ndeye 200 μl, iyo yakakurudzirwa elution vhoriyamu ndeye 80μl.
Sarudzo 2.
Yakakurudzirwa Kubvisa Kititi: Macro & Micro & Micro-Test Sampula Sunungura Reagent (HWTS-3005-8), inofanira kubviswa zvakanyanyisa maererano nemirairo.
Sarudzo3.
Yakakurudzirwa Kubvisa Kititi: Qiaamp VIRAL RNA MINI KIT (52904) kana Nucleic acid yekubvisa kana kuchenesa kit (ydp315-r), inofanira kubviswa mukuraira kwakasimba nemirairo. Iyo sampuli vhoriyamu ndeye 140 μL, iyo yakakurudzirwa elution vhoriyamu ndeye60μl.
