Enterovirus 71 (EV71)
Chigadzirwa Zita
HWTS-EV003- Entererovirus 71 (EV71) Nucleic acid Kuonekwa Kit (Fluolescence PCR)
Epidemiology
Hosha-tsoka-yemuromo chirwere chirwere chinotapukira chinokonzerwa negumi entroviruse (Ev). Parizvino, marudzi 108 eSerotypes emakore emakore akawanikwa, ayo akakamurwa mumapoka mana: A, b, c naD. Entertrovus ev71 uye Coxa16 ndiwo mainter. Chirwere ichi chinonyanya kuitika muvana vari pasi pemakore mashanu, uye zvinogona kukonzera herpes pamaoko, tsoka, muromo uye zvimwe mumigwagwa. Nhamba diki yevana inokudziridza matambudziko akadai saMyOcararditis, Pulmonary Edema, uye Aseptic Miningoentolitis.
Chiteshi
| Nhamo | Ev71 |
| Rox | Kutonga kwemukati |
Technical parameter
| Chengetedza | ≤-18 ℃ |
| Shefu-Hupenyu | 9 mwedzi |
| Type Type | Orophayengeal Swab,Herpes fluid |
| Ct | ≤35 |
| CV | <5.0% |
| Lod | 500copiji / ml |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva pcr system Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRC Kuonekwa Systems Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Sarudzo 1.
Yakakurudzirwa Reagent: Macro & micro & micro-bvunzo sampula sununguko reagent (HWTS-3005-8), uye kubviswa kunofanirwa kuitiswa maererano nemirairo yekushandiswa kwekushandisa. Iyo inobviswa samples ndeye orophaaryngeal swabs kana herpes fluid samples kubva kune varwere vakaunganidzwa pane saiti. Wedzera iyo yakaunganidzwa macro & micro-bvunzo sampula sununguko reagent yemuenzaniso wega wega.
Sarudzo 2.
Yakakurudzirwa Reagent Reagent: Macro & Micro & Micro-Test General DNA / rWTS-3017-50, HWTS-3017-98, Otomatiki Acleic acid Extractor (HWTS-3006C, HWTS-3006B)) NaJiangsu Macro & Micro-Test Med-Tech Co, Ltd., uye kubviswa kunofanirwa kuitiswa zvinoenderana nemirairo yekushandiswa. Iyo yakabviswa sampuli vhoriyamu ndeye 200μl, uye iyo yakakurudzirwa elution vhoriyamu ndeye 80μl.
Sarudzo3.
Yakakurudzirwa Reagent Reagent: Qiaamp VIRAL RNA KIT (52904) NaQiagen kana Tiagen turus DNA / rna Kit (YDP315-R), uye kubviswa kunofanirwa kuitwa mukuraira kwakasimba nemirairo yekushandiswa. Iyo inobviswa sampuli vhoriyamu ndeye 140μL, uye iyo Yakakurudzirwa Vhoriyamu Vhoriyamu ndeye60μL.
