Enterovirus 71 (EV71)
Zita rechigadzirwa
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chirwere chemuromo weruoko chirwere chinotapukira chinokonzerwa nema enteroviruses (EV). Parizvino, marudzi 108 e enteroviruses akawanikwa, ayo akakamurwa kuita mapoka mana: A, B, C naD. Pakati pawo, enterovirus EV71 naCoxA16 ndiwo hutachiona hukuru. Chirwere ichi chinowanzoitika kuvana vari pasi pemakore mashanu, uye chinogona kukonzera herpes pamaoko, tsoka, muromo nedzimwe nzvimbo. Vana vashoma vanozosangana nematambudziko akadai se myocarditis, pulmonary edema, uye aseptic meningoencephalitis.
Chiteshi
| FAM | EV71 |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Chinobvisa muromo,Mvura yeHerpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | Makopi 500/mL |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Masisitimu ePCR e480 Enguva Yechokwadi Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Chinorayirwa kubvisa mushonga: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), uye kubviswa kwacho kunofanira kuitwa zvichienderana nemirairo yekushandisa. Mienzaniso yakatorwa ioropharyngeal swabs kana herpes fluid samples kubva kuvarwere vakatorwa panzvimbo yacho. Isa ma swabs akaunganidzwa muMacro & Micro-Test Sample Release Reagent (HWTS-3005-8) zvakananga, vortex wosanganisa zvakanaka, isa patembiricha yemukamuri kwemaminitsi mashanu, bvisa wozoichinja kuti isanganiswe zvakanaka, kuti uwane RNA yemuenzaniso wega wega.
Sarudzo yechipiri.
Reagent inokurudzirwa yekuburitsa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd., uye kuburitsa kunofanirwa kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakabviswa i200µL, uye vhoriyamu yekudzikisa inokurudzirwa i80µL.
Sarudzo 3.
Chinorayirwa kubvisa: QIAamp Viral RNA Mini Kit (52904) neQIAGEN kana TIANamp Virus DNA/RNA Kit (YDP315-R), uye kubvisa kunofanira kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakatorwa i140μL, uye vhoriyamu yekubvisa inokurudzirwa i60μL.
