Adenovirus Yese
Zita rechigadzirwa
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Human adenovirus (HAdV) ndeyerudzi rweMammalian adenovirus, inova hutachiona hweDNA hune tambo mbiri husina chinhu. MaAdenovirus akawanikwa kusvika pari zvino anosanganisira mapoka 7 (AG) uye marudzi 67, ayo 55 serotypes anokonzera chirwere kuvanhu. Pakati pawo, zvinogona kukonzera hutachiona hwemudumbu ndeizvi: boka B (Types 3, 7, 11, 14, 16, 21, 50, 55), Boka C (Types 1, 2, 5, 6, 57) uye Boka E (Type 4), uye zvinogona kukonzera hutachiona hwemanyoka mudumbu ndiBoka F (Types 40 na 41) [1-8]. Mhando dzakasiyana dzine zviratidzo zvakasiyana zvekiriniki, asi kunyanya hutachiona hwemudumbu. Zvirwere zvekufema zvinokonzerwa nehutachiona hwemudumbu hwevanhu zvinokonzera 5% ~ 15% yezvirwere zvekufema zvepasi rose, uye 5% - 7% yezvirwere zvekufema zvevana pasi rose [9]. Adenovirus inowanikwa munzvimbo dzakasiyana-siyana uye inogona kutapukirwa gore rose, kunyanya munzvimbo dzine vanhu vakawanda, idzo dzinowanzo sangana nezvirwere zvemuno, kunyanya muzvikoro nemumisasa yemauto.
Chiteshi
| FAM | adenovirus yepasi roseasidhi ye nucleic |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Chinobvisa ganda mumhino，Chinoputira pahuro |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 300/mL |
| Kunyatsojeka | a) Edza mareferensi asina kunaka ekambani akagadzirwa nekiti, uye mhedzisiro yebvunzo inosangana nezvinodiwa. b) Shandisa kit iyi kuti uone kana pasina hutachiona hunobatanidzwa nezvimwe zvirwere zvekufema (zvakaita seInfluenza A virus, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, nezvimwewo) kana mabhakitiriya (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, nezvimwewo). |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadis (FQD-96AHangzhouTekinoroji yeBioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) BioRad CFX96 Masisitimu ePCR eNguva Yechokwadi, BioRad CFX Opus 96 Masisitimu ePCR eNguva Yechokwadi |
Kufamba Kwebasa
(1) Zvinokurudzirwa kuti mushonga wekuburitsa ushande:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kubviswa kwesampuro kunofanirwa kuitwa zvichienderana nemirairo. Sampuro inotorwa ndeyevarwere'sampuro dzemushonga wepamuromo kana wepahuro dzakaunganidzwa panzvimbo yacho. Isa sampuro mumudziyo unoburitsa sampuro neJiangsu Macro & Micro-Test Med-Tech Co., Ltd., kuti usanganise zvakanaka, isa patembiricha yemukamuri kwemaminitsi mashanu, bvisa wozodziisa wodzisanganisa zvakanaka kuti uwane DNA yesampuro yega yega.
(2) Zvinokurudzirwa kuti mushonga wekuburitsa ushande:Macro & Micro-Test Kutapukira Kiti yeDNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kuvhiya uku kunofanira kuitwa zvichienderana nemirairo. Vhoriyamu yemuenzaniso yakatorwa i200μLuyevhoriyamu yemhinduro inokurudzirwais80μL.
(3) Chinorayirwa kubvisa: Nucleic Acid Extraction kana Purification Reagent (YDP)315) naTiangen Biotech (Beijing) Co.,Ltd., iyoKushanda kunofanira kuitwa zvichienderana nemirairo. Vhoriyamu yemuenzaniso yakatorwa i200μLuyevhoriyamu yemhinduro inokurudzirwais80μL.
