Adenovirus Universal
Product name
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Human adenovirus (HAdV) ndeye genus Mammalian adenovirus, inova hutachiona hweDNA hukapetwa kaviri husina hamvuropu.Adenoviruses akawanikwa kusvika ikozvino anosanganisira 7 subgroups (AG) uye 67 marudzi, ayo 55 serotypes ari pathogenic kuvanhu.Pakati pavo, zvinogona kutungamira kuhutachiona hwekufema turakiti kunyanya boka B (Types 3, 7, 11, 14, 16, 21, 50, 55), Boka C (Types 1, 2, 5, 6, 57) uye Boka E. (Type 4), uye inogona kutungamirira kuhutachiona hwemanyoka emudumbu iboka F (Marudzi 40 ne41)[1-8].Mhando dzakasiyana dzine zviratidzo zvakasiyana zvekiriniki, asi kunyanya zvirwere zvekufema.Zvirwere zvekufema zvinokonzerwa nezvirwere zvekufema zvemuviri wemunhu zvinotora 5% ~ 15% yezvirwere zvekufema zvepasi rose, uye 5% -7% yezvirwere zvekufema kwevana pasi rose[9].Adenovirus inopararira munzvimbo dzakasiyana-siyana uye inogona kutapukirwa gore rose, kunyanya munzvimbo dzine vanhu vakawanda, dzinowanzoita kuputika kwenzvimbo, kunyanya muzvikoro uye mumisasa yemauto.
Channel
| FAM | adenovirus universalnucleic acid |
| ROX | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | Nasopharyngeal swab,Huro swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Makopi/mL |
| Zvakananga | a) Edza iyo yakamisikidzwa kambani yakaipa mareferensi nekiti, uye bvunzo mhedzisiro inosangana nezvinodiwa. b) Shandisa kit iyi kuona uye hapana kuyambuka-reactivity nemamwe hutachiona hwekufema (senge Influenza A virus, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, etc.) kana mabhakitiriya (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, nezvimwewo). |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection Systems (FQD-96A, HangzhouBioer tekinoroji) MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Chaiyo-Nguva PCR Systems, BioRad CFX Opus 96 Chaiyo-Nguva PCR Systems |
Kuyerera Kwebasa
(1) Inokurudzirwa kuburitsa reagent:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Kubvisa kunofanira kuitwa maererano nemirairo.Muenzaniso wakabudiswa ndiwo varwere'nasopharyngeal swab kana huro swab samples dzakaunganidzwa panzvimbo.Wedzera iwo masampula muyeyedzo yekuburitsa reagent naJiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kusanganisa zvakanaka, isa pamhepo tembiricha kwemaminetsi mashanu, tora kunze uye wozopinza uye sanganisa zvakanaka kuti uwane iyo DNA. sampuli imwe neimwe.
(2) Inokurudzirwa kuburitsa reagent:Macro & Micro-Muedzo Viral DNA/RNA Kit(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).kuvhiyiwa kunofanira kuitwa zvakanyatsoenderana nemirairo.Iyo yakabudiswa sampuli yevhoriyamu ndeye 200μL,uyeyakakurudzirwa elution volumeis80μL.
(3) Inokurudzirwa kudhirowa reagent: Nucleic Acid Extraction kana Purification Reagent (YDP315) neTiangen Biotech (Beijing) Co., Ltd., ivooparesheni inofanira kuitwa zvakanyatsoenderana nemirairo.Iyo yakabudiswa sampuli yevhoriyamu ndeye 200μL,uyeyakakurudzirwa elution volumeis80μL.
