Mhando makumi maviri nemasere dzeHuman Papilloma Virus ine njodzi huru (16/18 Typeing) Nucleic Acid
Zita rechigadzirwa
HWTS-CC006A-28 Mhando dzeHuman Papilloma Virus ine njodzi huru (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Gomarara remumuromo wechibereko ndeimwe yemabundu anonyanya kuipa munhengo dzekubereka dzevakadzi. Zvidzidzo zvakaratidza kuti hutachiona hweHPV hunoramba huripo uye hutachiona hwakawanda ndizvo zvimwe zvezvikonzero zvikuru zvegomarara remumuromo wechibereko. Parizvino, mishonga inoshanda inozivikanwa ichiri kushaikwa yegomarara remumuromo wechibereko rinokonzerwa neHPV, saka kuwanikwa nekukurumidza uye kudzivirira hutachiona hwemumuromo wechibereko hunokonzerwa neHPV ndiyo kiyi yekudzivirira gomarara remumuromo wechibereko. Zvakakosha zvikuru kugadzira bvunzo iri nyore, chaiyo uye inokurumidza yekuongorora chirwere chegomarara remumuromo wechibereko kuti rionekwe uye rirapwe.
Chiteshi
| Musanganiswa weKupindura | Chiteshi | Rudzi |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Kudzora Kwemukati | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Chinokwesha Mumuromo wechibereko, Chinokwesha Munhengo Yemukadzi, Weti |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | Makopi 300/mL |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinorayirwa chekuburitsa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Wedzera 200μL yemunyu wakajairika kuti udzorere pellet padanho re2.1, uye wobva wabvisa zvichienderana nemirayiridzo yekushandisa reagent iyi yekuburitsa. Vhoriyamu yekubvisa inokurudzirwa i80μL.
Chinorayirwa chekuburitsa mvura chinokurudzirwa: QIAamp DNA Mini Kit (51304) kana Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Wedzera 200μL yemunyu wakajairika kuti udzorerezve pellet iri padanho re2.1, wobva wabvisa mvura yacho zvichienderana nemirayiridzo yekushandisa chinobvumirwa ichi chekuburitsa mvura. Vhoriyamu yesamples yakabviswa yese i200μL, uye vhoriyamu yekudzikisa mvura i100μL.
