28 Mhando dzeKukuru-Kukwira Human Papilloma Virus (16/18 Typing) Nucleic acid
Chigadzirwa Zita
HWTS-CC006A-28 Mhando dzeKukwikwidza Human Papilloma Virus (16/18 Typing) Nucleic acid Kuonekwa Kit (Fluorescence PCR)
Epidemiology
Cervical cancer ndeimwe yemataurirwo akaomesesa anowoneka weturakiti remukadzi rekubereka. Zvidzidzo zvakaratidza kuti HPV kuramba vachirwara nehutachiona uye hutachiona hwakawanda nderimwe rechikonzero chikuru chekanza yechibereko. Parizvino, marapirwo anozivikanwa achiri kushaya cancer yakakonzerwa neHPV, saka kuwanikwa kwekutanga uye kudzivirira kwechibereko kwakakonzerwa neHPV ndiyo kiyi yekudzivirira cancer yechibereko. Izvo zvakakosha kwazvo kumisikidza iri nyore, yakatarwa uye inokurumidza etiology yekuongorora yekuongororwa kwekiriniki uye kurapwa kwegomarara rekanza.
Chiteshi
| Reaction musanganiswa | Chiteshi | Nyora |
| PCR-Mix1 | Nhamo | 18 |
| Vic (Hex) | 16 | |
| Rox | 31, 33, 35, 39, 45, 52, 52, 58, 58, 69, 68 | |
| CY5 | Kutonga kwemukati | |
| PCR -MiX2 | Nhamo | 6, 11, 54, 83 |
| Vic (Hex) | 26, 44, 61, 81 | |
| Rox | 40, 42, 43, 53, 73, 83 | |
| CY5 | Kutonga kwemukati |
Technical parameter
| Chengetedza | Mvura: ≤-18 ℃ |
| Shefu-Hupenyu | Mwedzi gumi nemaviri |
| Type Type | Cervical swab, vaginal swab, urini |
| Ct | ≤28 |
| CV | <5.0% |
| Lod | 300copiji / ml |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva pcr system Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRR Kuongororwa system Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Yakakurudzirwa Reagent Reagent: Macro & Micro & Micro-Test Virara / rna Kit (HWTS-3017-50, HWTS-3017-98) (iyo inogona kushandiswa nemacro & micro-bvunzo Otomatiki Acleic acid Extractor (HWTS-3006C, HWTS-3006B)) NaJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Wedzera 200μL yeyakajairika saline kuti udzorere pellet in the stepting Iyo Yakakurudzirwa Elution Vhoriyamu ndeye 80μL.
Yakakurudzirwa Reagent Reagent: QiaApp DNA Mini Kit (51304) kana macro & micro-test virra / rna column (hwts-3020-50). Wedzera 200μL yeyakajairwa saline kuti uendese pellet mudanho 2.1, uyezve kuongororwa kunofanirwa kuitiswa maererano nemirairo yekushandisa iyi yekuwedzera Iyo yakabviswa sampuli vhoriyamu yemasampuli ese mazana maviri nemakumi mairi 200μl, uye iyo yakakurudzirwa vhoriyamu vhoriyamu ndeye 100μl.
