Mhando gumi neshanu dzeHuman Papillomavirus E6/E7 Gene mRNA ine njodzi huru
Zita rechigadzirwa
HWTS-CC005A-15 Mhando dzeHuman Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Epidemiology
Gomarara remuchibereko ndeimwe yemhando dzegomarara revakadzi dzakajairika pasi rose, uye kuwanikwa kwaro kwakabatana zvakanyanya nehutachiona hwema papillomavirus (HPV), asi chikamu chidiki chete chehutachiona hweHPV chinogona kukura kuita gomarara. HPV ine njodzi huru inokanganisa masero emudumbu uye inogadzira mapuroteni maviri e oncoprotein, E6 neE7. Puroteni iyi inogona kukanganisa mapuroteni akasiyana-siyana emasero (akadai semapuroteni e tumor suppressor pRB ne p53), kuwedzera nguva yemasero, kukanganisa kugadzirwa kweDNA uye kugadzikana kwe genome, uye kukanganisa mhinduro dzekudzivirira hutachiona uye dzinorwisa bundu.
Chiteshi
| Chiteshi | Chikamu | Kuedzwa kweGenotype |
| FAM | HPV Reaction Buffer 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Jini remunhu reβ-actin | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Jini reINS remunhu |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Kubvisa muromo wemuromo wechibereko |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Makopi 500/mL |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-TimeMaitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinorayirwa kubvisa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nemirairo yekushandisa. Vhoriyamu yekubvisa inokurudzirwa i50μL. Kana sampuro isina kugayiwa zvakakwana, idzosere kudanho rechina kuti idzorerwe. Wobva waedza zvichienderana nemirairo yekushandisa.
Reagent inokurudzirwa yekuburitsa: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Kubvisa kunofanirwa kuitwa zvichienderana nemirairo yekushandisa zvakanyanya (Mudanho rechishanu, wedzera kaviri huwandu hwemhinduro inoshanda yeDNaseI, kureva kuti, tora 20μL yeRNase-Free DNaseI (1500U) stock solution muchubhu itsva yeRNase-Free centrifuge, wedzera 60μL yeRDD buffer, wosanganisa zvinyoro nyoro). Vhoriyamu yekubvisa inokurudzirwa i60μL. Kana sampuro isina kugayiwa zvakakwana, idzosere kudanho rechishanu kuti idzorerwe. Wobva waedza zvichienderana nemirairo yekushandisa.
