15 Mhando dzeHuman Papillomavirus E6/E7 Gene mRNA
Product name
HWTS-CC005A-15 Mhando dzeHuman Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Epidemiology
Chirwere chegomarara remuchibereko nderimwe remhando dzegomarara remunhukadzi pasi rose, uye kuitika kwaro kune hukama hwepapillomaviruses (HPV), asi chikamu chidiki cheutachiona hweHPV chinogona kuita gomarara. High-risk HPV inobata cervical epithelial cells uye inogadzira maoncoprotein maviri, E6 uye E7. Iyi puroteni inogona kukanganisa akasiyana-siyana mapuroteni emasero (akadai sebundu suppressor mapuroteni pRB uye p53), kurebesa kutenderera kwesero, kukanganisa DNA synthesis uye genome kugadzikana, uye kukanganisa antiviral uye antitumor immune mhinduro.
Channel
| Channel | Chikamu | Genotype yakaedzwa |
| FAM | HPV Reaction Buffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Munhu β-actin gene | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Munhu INS gene |
Technical Parameters
| Storage | Mvura: ≤-18℃ |
| Sherufu-hupenyu | 9 mwedzi |
| Type Type | Cervical swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Makopi/mL |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR SystemApplied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanira kuitwa zvakanyatsoenderana nemirairo yekushandisa. Iyo yakakurudzirwa elution vhoriyamu i50μL. Kana sampuli isina kunyatsogayiwa, idzorere kudanho rechina rekugaya zvakare. Uye wozoyedza zvinoenderana nemirairo yekushandisa.
Inokurudzirwa kuburitsa reagent: RNAprep Yakachena Mhuka Tissue Yese RNA Extraction Kit (DP431). Kubvisa kunofanirwa kuitwa maererano nemirairo yekushandisa zvakasimba (Mudanho rechishanu, kaviri kuwanda kweDNaseI kushanda mhinduro, kureva, tora 20μL yeRNase-Free DNaseI (1500U) stock solution muRNase-Free centrifuge chubhu, wedzera 60μL yeRDD buffer, uye sanganisa zvinyoro nyoro). Iyo yakakurudzirwa elution vhoriyamu i60μL. Kana sampuli isina kunyatsogayiwa, idzorere kune nhanho yechishanu yekugaya zvakare. Uye wozoyedza zvinoenderana nemirairo yekushandisa.
