15 Mhando dzeWepamusoro-dzirimo Human Papillomavirus E6 / E7GE GUN MTNA
Chigadzirwa Zita
HWTS-CC005A-15 Mhando dzeKukwikwidza-njodzi Human Papillomavirus E6 / E7 Gene MrNN Kuona Kit (Fluorescence PCR)
Epidemiology
Cervical cancer ndeimwe yemhando dzekenza dzechikadzi pasi rese, uye chiitiko chavo zvine hukama zvakanyanya neyepapa. Yakanyanya-njodzi HPV inokanganisa cervical epithelial masero uye inoburitsa maviri Oncopolitoteins, e6 uye e7. Iyi protein inogona kukanganisa mhando dzakasiyana dzemutauro (dzakadai sepuroteni yekudzvinyirira prb uye p53), inowedzera cell stynthesis uye genome kugadzikana uye antiveral uye antitumor immune mhinduro.
Chiteshi
| Chiteshi | Chikamu | Genotype Yakaedzwa |
| Nhamo | HPV reaction buffer 1 | HPV16,31,33,35,5,55,52,58 |
| Vic / Hex | Human β-Actin Gene | |
| Nhamo | Hpv reaction buffer 2 | HPV 18,39,45,45,53,5,59,66,68 |
| Vic / Hex | HUVANI VANHU Gumi |
Technical parameter
| Chengetedza | Mvura: ≤-18 ℃ |
| Shefu-Hupenyu | 9 mwedzi |
| Type Type | Cervical swab |
| Ct | ≤38 |
| CV | <5.0% |
| Lod | 500 makopi / ml |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR systemYakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva pcr system Lightcycler ®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRR Kuongororwa system Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Yakakurudzirwa Reagent Reagent: Macro & Micro & Micro-test virra DNA / rwts-3020-50-test-test med-tech inofanirwa kuitwa zvakanyanyisa zvinoenderana nemirairo yekushandisa . Iyo Yakakurudzirwa Elution Vhoriyamu ndeye 50μl. Kana iyo sampuro isina kunyatsocherwa, dzosera iyo nhanho 4 yekudzosera mari. Uye wobva waedza maererano nemirairo yekushandiswa.
Yakakurudzirwa Regency Reagent: RNAPREP Yakachena Yemhuka Tishu Yese RNA Exertction Kit (DP431). Kuongororwa kunofanirwa kuitiswa maererano nemirairo yekushandiswa zvakanyanyisa (mudanho rechisimba Wedzera 60μL yeRdd buffer, uye sanganisa zvinyoro-nyoro). Iyo Yakakurudzirwa Elution Vhoriyamu ndeye60μl. Kana iyo sampuro isina kunyatsocherwa, dzosera iyo nhanho 5 yekudzosera mari. Uye wobva waedza maererano nemirairo yekushandiswa.
