Virusi reEbola reZaire
Zita rechigadzirwa
HWTS-FE008 Kiti yeKuona Nucleic Acid yeZaire Ebola Virus (Fluorescence PCR)
Epidemiology
Virusi reEbola ndereFiloviridae, inova vhairasi reRNA risina tambo imwe chete risina tambo. Mavhairasi imhando refu ine avhareji yehurefu hwevirion hwe1000nm uye dhayamita inosvika 100nm. Genome revhairasi reEbola iRNA isina tambo isina tambo ine saizi ye18.9kb, ine mapuroteni manomwe emuviri uye puroteni imwe chete isina structural. Vhairasi reEbola rinogona kukamurwa kuita mhando dzakadai seZaire, Sudan, Bundibugyo, Tai Forest neReston. Pakati padzo, mhando yeZaire nerudzi rweSudan zvinonzi ndizvo zvinokonzera kufa kwevanhu vazhinji nekuda kwehutachiona. EHF (Ebola Hemorrhagic Fever) chirwere chinotapukira chinobuda ropa chinokonzerwa nevhairasi reEbola. Vanhu vanonyanya kutapukirwa nekusangana nemvura yemuviri, tsvina uye tsvina yevarwere kana mhuka dzine hutachiona, uye zviratidzo zvekiriniki zvinonyanya kubuda fivha, kubuda ropa uye kukuvara kwenhengo dzakawanda. EHF ine huwandu hwakawanda hwerufu hwe50%-90%.
Chiteshi
| FAM | asidhi ye nucleic yeMP |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Seramu itsva, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/μL |
| Kunyatsojeka | Shandisa makits kuti uedze mareferensi asina kunaka ekambani, mhedzisiro yacho inosangana nezvinodiwa. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Real-Time, uye Sisitimu yePCR yeBioRad CFX Opus 96 Real-Time |
Kufamba Kwebasa
Sarudzo 1.
Chinorayirwa chekuburitsa mvura chinokurudzirwa: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction kana Purification Reagent (YDP315-R) neTiangen Biotech (Beijing) Co., Ltd. Chinofanira kubviswa zvichienderana nemirairo, uye huwandu hunokurudzirwa hwekuburitsa mvura i140μL uye huwandu hunokurudzirwa hwekuburitsa mvura i60μL.
Sarudzo yechipiri.
Chinoshandiswa pakuburitsa zvinhu chinokurudzirwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Inofanira kubviswa zvichienderana nemirairo. Vhoriyamu yekubvisa zvinhu i200μL, uye vhoriyamu yekudzikisa zvinhu i80μL.
Sarudzo 3.
Zvinokurudzirwa kuti zvibudiswe: Nucleic Acid Extraction Reagent (1000020261) uye High Throughput Automated Sample Preparation System (MGISP-960) neBGI zvinofanira kuburitswa zvichienderana nemirairo. Vhoriyamu yekubvisa i160μL, uye vhoriyamu yekudzikisa inokurudzirwa i60μL.
