Zaire Ebola Virus
Chigadzirwa Zita
HWTS-Fe008 Zaire Ebola Virus Nucleic acid Kuwanikwa Kit (Fluorescence PCR)
Epidemiology
Utachiona hweEbola ndehweFiloviridiae, iro risingazivikanwe vasina kurovedzwa vasina hutachiona hwehutachiona hwehutachiona. Virmes inguva refu mafirita ane average virimi kureba kwe 1000nm uye dhayimendi yeanoda kusvika 100nm. Iyo EBOLA MARILE genome is is is is is is is is Strand rna ine saizi ye 18.9kb, encoding 7 marefu emapuroteni uye 1 isina-mraptral protein. Utachiona hweEbola hunogona kukamurwa kuva marudzi kunge zaire, Sudan, Bundibugyo, Tai Sango uye Reston. Pakati pavo, mhando yemhando yeZaire uye Sudan mhando yakataurwa kukonzera kufa kwevanhu vazhinji kubva kutapukirwa. EHF (Ebola Hemorrhag Chever) Icho chiitiko chakashata hupfu hutachiona hwakakonzerwa neVirivhi veEbola. Vanhu vanonyanya kutapukirwa nekuonana nemvura yemuviri, zvigadziko uye mhuka dzinotapukirwa, uye zviratidziro zvekiriniki zvinonyanya kuratidzira fivha, kubuda ropa uye akawanda organ. EHF ine huwandu hwakanyanya hwekufa hwe50% -90%.
Chiteshi
| Nhamo | MP Nucleic acid |
| Rox | Kutonga kwemukati |
Technical parameter
| Chengetedza | ≤-18 ℃ |
| Shefu-Hupenyu | 9 mwedzi |
| Type Type | Serum nyowani, plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | Makumi mashanu emakopi / μL |
| Chokwadi | Shandisa iyo kitsi kuidza pfungwa dzisina kunaka dzekambani, mhedzisiro yacho inosangana nezvinodiwa. |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva PCR system (Hongshi Medical Technology Co, Ltd.) Lightcycler ®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PCR Kuongororwa System (FQD-96A, Hangzhou Bioer Technology) Ma-6000 chaiyo-nguva quolitative thermal cyler (Suzhou molarray co., Ltd.) Biorad cfx96 chaiyo-nguva PCR system, uye Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Sarudzo 1.
Yakakurudzirwa Reagent Reagent: Qiaamp VIRAL RNA MINI KIT (52904), Nucleic acid yekubvisa kana kuchenesa Reagent (YDP315-R) naTijen Biotech (Beijing) Co, LTD. Inofanirwa kubviswa mukurongeka kwakajairika kune iyo mirairo, uye iyo yakakurudzirwa vhoriyamu yemuenzaniso ndeye 140μL uye iyo yakakurudzirwa uye yakakurudzirwa elution vhoriyamu ndeye60μ.
Sarudzo 2.
Yakakurudzirwa Reaget Reagent: Macro & Micro & Micro-Test virra / rna Kit (HWTS-3004- 3004-96 & micro & micro & HWTS inofanira kubviswa maererano nemirairo. Iyo yekuwedzera sampuli vhoriyamu ndeye 200μl, uye iyo yakakurudzirwa elution vhoriyamu ndeye 80μl.
Sarudzo 3.
Yakakurudzirwa Regency Reagents: Nucleic Acid Extraction Regent (1000020261) uye yakakwira yekupfuura otomatiki yekugadzirira system (MGISP-960) naBGI inofanira kubviswa maererano nemirairo. Iyo vhoriyamu yekuwedzera ndeye 160μL, uye iyo yakakurudzirwa vhoriyamu vhoriyamu ndeye60μ.
