Enterococcus isingapindi muVancomycin uye Gene isingapindi muMishonga
Zita rechigadzirwa
HWTS-OT090-Vancomycin-resistant Enterococcus uye Kit yeKuona Majini Isingapindwe Nemishonga (Fluorescence PCR)
Epidemiology
Kuramba Mushonga kunozivikanwawo sekuramba mushonga, zvinoreva kuramba kwebhakitiriya kusimba kwemishonga inorwisa mabhakitiriya. Kana mishonga yangoramba mushonga, mhedzisiro yemishonga yechemotherapy inodzikira zvakanyanya. Kuramba Mushonga kunokamurwa kuita kuramba kwemukati uye kuramba kwakawanikwa. Kuramba kwemukati kunotsanangurwa nemajini ebhakitiriya echromosome, anopfuudzwa kubva kuchizvarwa kuenda kuchizvarwa, uye haachinje. Kuramba kwakawanikwa kunokonzerwa nekuti mushure mekusangana nemishonga inorwisa mabhakitiriya, mabhakitiriya anochinja nzira dzawo dzemuviri kuitira kuti asaurayiwe nemishonga inorwisa mabhakitiriya.
Majini ekudzivirira vancomycin eVanA neVanB anowanikwa asingade mishonga, pakati pawo VanA anoratidza mazinga akakwirira ekudzivirira vancomycin neteicoplanin, VanB inoratidza mazinga akasiyana ekudzivirira vancomycin, uye inonzwa teicoplanin. Vancomycin inowanzoshandiswa mukurapa hutachiona hweGram-positive, asi nekuda kwekubuda kwevancomycin-resistant enterococci (VRE), kunyanya enterococcus faecalis ne enterococcus faecium, inodarika 90%, yakaunza matambudziko matsva makuru ekurapa. Parizvino, hapana mushonga chaiwo wekurapa VRE. Uyezve, VRE inogonawo kutapurira majini ekudzivirira mishonga kune mamwe enterococci kana mamwe mabhakitiriya eGram-positive.
Chiteshi
| FAM | Enterococci isingapindi Vancomycin (VRE): Enterococcus faecalis uye Enterococcus faecium |
| VIC/HEX | Kudzora Kwemukati |
| CY5 | jini rekudzivirira vancomycin VanB |
| ROX | jini rekudzivirira vancomycin VanA |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | manyoka, ropa, weti kana makoroni chaiwo |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| Kunyatsojeka | Hapana mushonga unoshanda pamwe chete nezvimwe zvirwere zvekufema zvakaita se klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemolyticus, legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, kana kuti samples dzine mamwe majini asingagamuchirwe nemishonga CTX, mecA, SME, Samples dzeSHV neTEM. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi |
Kufamba Kwebasa
Zvinokurudzirwa kuti zvibudiswe: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
