Furuwenza yeSARS-CoV-2 furuwenza A furuwenza B Nucleic Acid Yakabatanidzwa
Zita rechigadzirwa
HWTS-RT060A-SARS-CoV-2 furuwenza A furuwenza B Nucleic Acid Combined Detection Kit (Fluorescence PCR)
Chitupa
AKL/TGA/CE
Epidemiology
Chirwere cheCoronavirus 2019 (COVID-19) chinokonzerwa neSARS-CoV-2 inova yerudzi rwe β Coronavirus. COVID-19 chirwere chinotapukira chinopararira mukufema, uye vanhu vazhinji vanowanzo batwa nechirwere ichi. Parizvino, varwere vane SARS-CoV-2 ndivo vanonyanya kutapurira chirwere ichi, uye varwere vasina zviratidzo vanogonawo kutapurira chirwere ichi. Zvichienderana nekuferefeta kuri kuitwa pari zvino, nguva yekuzvitakura imazuva 1-14, kazhinji mazuva 3-7. Zviratidzo zvikuru zvaive fivha, kukosora kwakaoma uye kuneta. Varwere vashoma vane zviratidzo zvakaita sekuzara kwemhino, mhino inoyerera, pahuro panorwadza, myalgia uye manyoka.
Furuwenza chirwere chekufema chinokonzerwa nevhairasi yefuruwenza. Chinotapukira zvakanyanya uye chinopararira zvakanyanya kuburikidza nekukosora nekuhotsira. Chinowanzo pararira muchirimo nemuchando. Kune mhando nhatu dzefuruwenza, Furuwenza A (IFV A), Furuwenza B (IFV B) uye Furuwenza C (IFV C), dzese dziri dzemhuri ye ortomyxovirus. Furuwenza A neB, dziri mavhairasi eRNA ane tambo imwe chete, ndiwo anonyanya kukonzera zvirwere zvevanhu. Furuwenza A chirwere chekufema chinotapukira, chinosanganisira H1N1, H3N2 nedzimwe mhando, chiri nyore kuchinja. Kupararira kwepasi rose, "shift" zvinoreva kuchinja kwefuruwenza A, zvichikonzera "subtype" itsva yehutachiona. Furuwenza B yakakamurwa kuita madzinza maviri: Yamagata neVictoria. Furuwenza B ine antigenic drift chete, uye inodzivisa kutariswa nekubviswa nemasoja emuviri evanhu kuburikidza nekuchinja. Asi mavhairasi efuruwenza B anoshanduka zvishoma nezvishoma kupfuura furuwenza A yevanhu, iyo inokonzerawo hutachiona hwekufema nezvirwere muvanhu.
Chiteshi
| FAM | SARS-CoV-2 |
| ROX | IFV B |
| CY5 | IFV A |
| VIC(HEX) | Majini ekudzora mukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ murima |
| Kupisa kweropa: ≤30℃ murima | |
| Nguva yekuzorora | Mvura: mwedzi 9 |
| Kugadziriswa kweropa: mwedzi gumi nemiviri | |
| Rudzi rwemuenzaniso | Zviputi zvemumhino, zviputi zvemumhino |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi mazana matatu/mL |
| Kunyatsojeka | Mhedzisiro yebvunzo yakaratidza kuti kit iyi yaishanda pamwe chete nehuman coronavirus SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respiratory syncytial virus A na B, parainfluenza virus 1, 2 na 3, rhinovirusA, B na C, adenovirus 1, 2, 3, 4, 5, 7 na 55, human metapneumovirus, enterovirus A, B, C na D, human cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata Pakanga pasina kusangana pakati pePneumocystis yersini neCryptococcus neoformans. |
| Zvishandiso Zvinoshanda: | Inogona kuenzana nemidziyo yePCR inopenya iri pamusika. Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Zvinokurudzirwa kuti munhu abvise reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Sarudzo yechipiri.
Chinorudzirwa chekubvisa: Nucleic Acid Extraction kana Purification Reagent (YDP302) chakagadzirwa neTiangen Biotech (Beijing) Co., Ltd.
