Chombo Chitsva cheTuberculosis Diagnosis uye Kuramba Kwezvinodhaka: A New Generation Targeted Sequencing (tNGS) Yakasanganiswa neMuchina Kudzidza kweTuberculosis Hypersensitivity Diagnosis.
Literature report: CCa: a diagnostic model based on tNGS uye muchina kudzidza, iyo yakakodzera vanhu vane shoma bhakitiriya tuberculosis uye tuberculous meningitis.
Musoro weThesis: Tuberculous-yakanangana nechizvarwa chinotevera kutevedzana uye kudzidza muchina: an Ultra-sensitive diagnostic zano repaucific pulmonary tubulars uye tubular meningitis.
Periodical: 《Clinica Chimica Acta》
IF:6.5
Zuva rekubudiswa: Ndira 2024
Yakasanganiswa neYunivhesiti yeChinese Academy yeScience uye Beijing Chest Hospital yeCapital Medical University, Macro & Micro-Test yakagadzira tuberculosis yekuongororwa modhi yakavakirwa pachizvarwa chitsva cheyakanangwa kutevedzana (tNGS) tekinoroji uye nzira yekudzidza muchina, iyo yakapa yakanyanya-yakakwirira. kuona kunzwisiswa kwetibhii ine mabhakitiriya mashoma uye tuberculous meningitis, yakapa nzira itsva yekuongorora hypersensitivity yekuongororwa nekiriniki yemhando mbiri dzetibhii, uye yakabatsira kuongororwa kwechokwadi, kuona kusaramba kwezvinodhaka uye kurapwa kwechirwere cherurindi.Panguva imwecheteyo, zvinowanikwa kuti plasma yemurwere cfDNA inogona kushandiswa seyakakodzera mhando yemhando yekliniki sampling mukuongororwa kweTBM.
Muchidzidzo ichi, 227 plasma samples uye cerebrospinal fluid samples dzakashandiswa kumisikidza maviri ekiriniki cohorts, umo marabhoritari diagnostic cohort samples akashandiswa kumisikidza muchina wekudzidza modhi yechirwere cherurindi, uye kiriniki yekuongorora cohort samples yakashandiswa kuratidza iyo yakagadzwa. diagnostic model.Ese masampuli akatanga kunangana nedziva rakanangwa rakanangwa rekuferefeta reMycobacterium tuberculosis.Zvadaro, zvichibva pane TB-tNGS yekuenzanisa data, muenzaniso wemuti wemusarudzo unoshandiswa kuita 5-fold cross-validation pakudzidziswa uye kusimbiswa kweseti yerabhoritari yekuongorora mutsara, uye mizinga yekuongorora yeplasma samples uye cerebrospinal fluid samples inowanikwa.Chikumbaridzo chakawanikwa chinounzwa mumaseti maviri ebvunzo dzekiriniki yekuongororwa mutsara kuti ionekwe, uye mashandiro ekuongorora emudzidzi anoongororwa neROC curve.Pakupedzisira, nzira yekuongorora yechirwere chetachiona yakawanikwa.
Fig. 1 schematic dhizaini yekutsvagisa dhizaini
Migumisiro: Zvinoenderana nematanho chaiwo eCSF DNA sample (AUC = 0.974) uye plasma cfDNA sample (AUC = 0.908) yakatemerwa muchidzidzo ichi, pakati pe227 samples, kunzwa kweCSF sampuro yaive 97.01%, iyo chaiyo yaive 95.65%, uye kunzwisiswa uye chaiyo yeplasma sampuli yaiva 82.61% uye 86.36%.Mukuongorora kwemakumi mana nemana akapetwa masampuli eplasma cfDNA uye cerebrospinal fluid DNA kubva kuvarwere veTBM, nzira yekuongorora yechidzidzo ichi ine kuenderana kwakanyanya kwe90.91% (40/44) muplasma cfDNA uye cerebrospinal fluid DNA, uye senitivity i95.45% (42/44).Muvana vane pulmonary tuberculosis, chirongwa chekuongorora chechidzidzo ichi chinonyanya kukoshesa sampuli dzeplasma kupfuura Xpert yekuona migumisiro yegastric juice samples kubva kuvarwere vakafanana (28.57% VS 15.38%).
Fig. 2 Ongororo yekushanda kwechirwere chetachiona chetachiona chemuenzaniso wehuwandu hwevanhu
Fig. 3 Diagnostic results of paired samples
Mhedziso: A hypersensitive diagnostic method for tuberculosis yakasimbiswa muchidzidzo ichi, iyo inogona kupa chigadziro chekuongorora nepamusoro-soro yekuona kunzwisiswa kwevarwere vekliniki vane oligobacillary tuberculosis (negative tsika).Kuonekwa kwe hypersensitive tuberculosis yakavakirwa paplasma cfDNA inogona kunge iri mhando yemuenzaniso yakakodzera kuongororwa kwetibhii inoshanda uye tuberculous meningitis (masampuli eplasma ari nyore kuunganidza pane cerebrospinal fluid kune varwere vanofungidzirwa kuti vane chirwere chepfungwa).
Nyaya yekutanga: https://www.sciencedirect.com/science/article/pii/s0009898123004990?kuburikidza ne%3Dihub
Kusuma kupfupi kweMacro & Micro-Test tuberculosis series zvigadzirwa zvekuongorora
Tichitarisa mamiriro ezvinhu akaoma emuenzaniso wevarwere vechirwere chetachiona uye zvinodiwa zvakasiyana, Macro & Micro-Test inopa yakakwana seti yeNGS mhinduro dzekubvisa liquefaction kubva kune sputum samples, Qualcomm raibhurari yekuvaka uye kutevedzana, uye kuongororwa kwedata.Zvigadzirwa izvi zvinovhara nekukurumidza kuongororwa kwevarwere vetibhii, kuona zvinodhaka kuramba tibhii, kutaipa mycobacterium tuberculosis uye NTM, hypersensitivity kuongororwa kwebhakitiriya-negative tuberculosis uye vanhu vane tuberculous, nezvimwe.
Seri yekuona kits yetibhii uye mycobacteria:
| Chinhu Nha | zita rechigadzirwa | chigadzirwa chekuongorora zvemukati | mhando yemuenzaniso | muenzaniso unoshanda |
| HWTS-3012 | Muenzaniso wekuburitsa mumiririri | Inoshandiswa mukurapa kwekunyungudika kwemasampuli esputum, yakawana rekodhi rekodhi nhamba, Sutong Machinery Equipment 20230047. | sputum | |
| HWTS-NGS-P00021 | Qualcomm quantity yekuongorora kit ye hypersensitive tuberculosis (probe capture nzira) | Kwete-invasive (liquid biopsy) hypersensitivity yekuona kwebhakitiriya-negative pulmonary tuberculosis uye mazamu europi;Mienzaniso yevanhu vanofungidzirwa kuti vane utachiona hwechirwere chetachiona kana kwete-tuberculosis mycobacteria yakaongororwa nepamusoro-yakadzika sequencing metagenomics, uye ruzivo rwekuona kuti tibhii kana kuti kwete-tuberculosis mycobacteria yakanga ine utachiona uye ruzivo rwekutanga rwekudzivirira zvinodhaka zve mycobacterium tuberculosis. vakapihwa. | Peripheral ropa, alveolar lavage fluid, hydrothorax uye ascites, tarisa puncture sample, cerebrospinal fluid. | Chizvarwa chechipiri |
| HWTS-NGS-T001 | Mycobacterium typing uye drug resistance kit (multiplex amplification sequencing nzira) | Mycobacterium typing test, kusanganisira MTBC uye 187 NTM;Kuonekwa kwechirwere cheMycobacterium tuberculosis kunobata mishonga gumi nemitatu uye nzvimbo gumi nenhanhatu dzekuchinja dzemajini ekuramba zvinodhaka. | Sputum, alveolar lavage fluid, hydrothorax uye ascites, tarisa puncture sample, cerebrospinal fluid. | Chechipiri/yechitatu chizvarwa dual platform |
Zvinonyanya kukosha: HWTS-NGS-T001 Mycobacterium kutaipa uye kutaipa kwemishonga yekurwisa zvinodhaka (multiplex amplification nzira)
Product sumo
Chigadzirwa chacho chinobva pamishonga mikuru yekutanga-yechipiri-mutsara inotsanangurwa mu WHO TB nhungamiro yekurapa, macrolides uye aminoglycosides inowanzoshandiswa mumitemo yekurapa yeNTM, uye nzvimbo dzekudzivisa zvinodhaka dzinovhara boka rimwe chete renzvimbo dzine chokuita nemishonga. WHO Mycobacterium tuberculosis complex mutation catalogue, pamwe chete nemamwe majini anonzi anoramba zvinodhaka uye nzvimbo dzekuchinja maererano nekuferefeta uye nhamba dzezvinyorwa zvepamusoro-soro kumba nekune dzimwe nyika.
Kuzivikanwa kwetaipa kunobva pamhando dzeNTM dzakapfupikiswa mugwaro reNTM rakaburitswa neChinese Journal yeTuberculosis uye Respiratory Diseases uye kubvumirana kwenyanzvi.Iwo akagadzirirwa kutaipa maprimers anogona kukudza, kutevedzana uye kududzira zvinopfuura zana nemakumi mapfumbamwe emarudzi eNTM.
Kuburikidza nekwakanangwa multiplex PCR amplification tekinoroji, iwo genotyping majini uye geno-resistant genes eMycobacterium akakwidziridzwa nemultiplex PCR, uye musanganiswa weamplicon wemajini anotangwa kuti aonekwe wakawanikwa.Zvigadzirwa zvakakwidziridzwa zvinogona kuvakwa kuita chizvarwa chechipiri kana chechitatu-chizvarwa chepamusoro-chepamusoro-chekutevedza maturakiti, uye ese echizvarwa chechipiri uye chechitatu-chizvarwa chinotevedzana mapuratifomu anogona kuiswa kune yakadzika-yakadzama sequencing kuti uwane ruzivo rwekutevedzana kwemajini anotangwa.Nokuenzanisa neanozivikanwa ekuchinja arimo mune yakavakirwa-mureferensi dhatabhesi (kusanganisira iyo WHO Mycobacterium tuberculosis complex mutation catalog uye hukama hwayo nekupokana nemishonga), shanduko dzine chekuita nekurwisa zvinodhaka kana kubatwa kwemishonga inorwisa tuberculosis yakatemwa.Yakasanganiswa neyakavhurika yega sputum sampuli yekurapa mhinduro yeMacro & Micro-Test, dambudziko rekushomeka kwenyucleic acid amplification yehunyanzvi hwekliniki sputum samples (yakapetwa kagumi kupfuura iyo yemaitiro echinyakare) yakagadziriswa, kuitira kuti kurongeka kwekurwisa zvinodhaka kunogona kutariswa. zvakananga kushandiswa kuchipatara sputum samples.
Kuonekwa kwechigadzirwa range
34zvinodhaka kuramba-zvinechekuita majini e18anti-tuberculosis mishonga uye6NTM mishonga yakaonekwa, yakavhara297nzvimbo dzekudzivisa zvinodhaka;Gumi marudzi eMycobacterium tuberculosis uye anopfuura190mhando dzeNTM dzakaonekwa.
Tafura 1: Ruzivo rwe18+6 Mishonga +190+NTM
Product advantage
Yakasimba kuchinjika kwekiriniki: iyo sputum samples inogona kutariswa zvakananga ne-self-liquefaction agent pasina tsika.
Iko kushanda kwekuyedza kuri nyore: denderedzwa rekutanga rekusimudzira kushanda kuri nyore, uye kuvakwa kweraibhurari kunopedzwa mumaawa matatu, izvo zvinovandudza kushanda zvakanaka.
Yakazara kutaipa uye kuramba zvinodhaka: kuvhara nzvimbo dzekutaipa uye dzekuramba zvinodhaka dzeMTB neNTM, anove mapoinzi akakosha ekiriniki, kutaipa kwakaringana uye kuona kuramba kwezvinodhaka, kutsigira yakazvimirira yekuongorora software, uye kugadzira mishumo yekuongorora nekudzvanya kumwe chete.
Kugarisana: kuenderana kwechigadzirwa, kuchinjika kune makuru ILM uye MGI/ONT mapuratifomu.
Product specification
| Product code | zita rechigadzirwa | Detection platform | specifications |
| HWTS-NGS-T001 | Mycobacterium kutaipa uye zvinodhaka kuramba yekuona kit (multiplex amplification nzira) | ONT, Illumina, MGI, Salus pro | 16/96rxn |
Nguva yekutumira: Jan-23-2024