Chombo chitsva cheBerberculosis Staagnosis uye Dishrance Kuona: Chizvarwa chitsva chakanangidzirwa kutevedzana
Mabhuku ezvinyorwa: CCA: mhando yekuongorora yakavakirwa paTNTs uye muchina kudzidza, izvo zvakakodzera vanhu vane hushoma hutachiona hwehutachiona uye tubberculous mentsitis.
THESIT Musoro: Turerculous
Nguva nenguva: "Clintica chimica actata"
Kana: 6.5
Zuva rebhuku racho: Ndira 2024
Yakasanganiswa neyunivhesiti yeChinese Chikoro cheSainzi uye Beijing Chest Chivatiro cheCity Medical Vunivhesiti Yevarwi Kuongororwa kunzwa kwe tuberculosis nevashoma mabhakitiriya uye tuberculous menningitis, yakapa itsva hypersensitivity diagnosis yeiyo Clinical kuongororwa kwemhando mbiri dzeTberculosis, uye akabatsira kuongororwa kwakarurama, kupokana kwezvinodhaka kuona uye kurapwa kwe tuberculosis. Panguva imwecheteyo, zvinowanikwa kuti iyo yemurwere plasma cfdna inogona kushandiswa semhando yakakodzera yemhando yekiriniki sampuli mune kuongororwa kweTBM.
Muchidzidzo ichi, 227 plasma samples uye cerebrobropinal samples dzaishandiswa kumisikidza mitezo miviri yekuverenga, uye kiriniki yekuongorora maitiro ekuita samples aishandiswa kuratidza kuti yakatangwa diatnostic mhando. Ma Samples ese akatanga kutariswa neakakomberedzwa akagadzirirwa chinangwa chekutora probe mudziva weMycobacterium Tuberculosis. Zvadaro, zvichibva paTB-tngs Sequencing data, iyo sarudzo yemuenzaniso inoshandiswa kuita mashanu-kupedzisa kuraira uye kusimbiswa kwerabhoritory queue, uye iyo yekuongorora mitezo yeiyo plasma samples uye cerebropinal fluid samples inowanikwa. Chikamu chakawanikwa chinounzwa mune maviri bvunzo seti yekiriniki quagnosis queue yekuona, uye kuita kwekuongorora kwemudzidzi kunoongororwa naRoc curve. Pakupedzisira, iyo yekuongorora muenzaniso weTaberculosis yakawanikwa.
Fig. 1 schematic dhizaini yekugadzira madimikira
Mhedzisiro: Sekureva kweiyo chaiyo thrinds of CSF DNA Sampuli (AUC = 0.908) Iko kunzwira tsitsi uye chokwadi cheplasma sampuli yaive 82.61% uye 86.36%. Mukuongorora kwemasampu makumi mana nemashanu. (42/44). Mune vana vane pulmonary trateculosis, iyo yekuongorora maitiro edzidzo iyi inotarisisa kune plasma samples kana iyo XPERT yekuona majusi egastric kubva kune vamwe varwere (28.57% vs 1538%).
Fig. 2 ongororo yekuongorora kweTberculosis diagnosis modhi yehuwandu hwehuwandu
Fig. 3 diagnostic mhedzisiro yemasampu akapetwa
Mhedziso: Iyo Hypersensitive nzira yekuongorora yeTberculosis yakamiswa muchidzidzo ichi, iyo inogona kupa chishandiso chekuongorora neyekutarisa kwekunzwa kwevarwere vekiriniki ne Oligobacillary tuberculosis (tsika dzisina kunaka). Kuonekwa kwe hypersensitive taberculosis kubva plasma cfdna inogona kunge iri sampule yemhando yekuongororwa kwechiSimbi uye tuberculous melenculosis.
Original Link: https://wwww.sciencedenirect.com/science/article/article/pii/s000/s0009898123004990? Via% 3dihub
Nhungamidzo pfupi yeMacro & micro-bvunzo tuberculosis Seriction zvigadzirwa
Tichifunga nezvematambudziko akaomesesa emhando yeTberculosis Zvigadzirwa zvinovhara nekukurumidza kweVarwere veBerculosis
Serial Kuona Kits yeTberculosis uye Mycobacteria:
| Chinhu kwete | Chigadzirwa Zita | Chigadzirwa Chigadzirwa Zvemukati | Sampuli mhando | Inoshanda Model |
| HWTS-3012 | Sample kuburitsa mumiriri | Inoshandiswa muLiquefriction kurapwa kweSputum samples, yakawana yekutanga-kirasi rekodhi nhamba, Sutong Machina Midziyo 20230047. | Sputum | |
| HWTS-NGS-PIBLE21 | Quastcom | Asiri-Invasive (mvura Biopsy) Hypersensitivity yekuona mabhakitiriya-isina kunaka pulmonary tuberlosis uye uropi nodules; Ma samples avanhu anofungidzirwa kutapukirwa ne tuberculosis kana isina-turberculososis zvakapihwa. | Ropa rePeripherel, Alveolar Lavage Fluid, Hydrothorax neAscites, tarisa punctor sampuli, cerebropinal fluid. | Chizvarwa chechipiri |
| HWTS-NG-T001 | Mycobacterium typing uye zvinodhaka kuramba kuona kit (Multiplex Amplization Sequencing nzira) | Mycobacterium typing test, inosanganisira MTBC uye 187 NTM;Kurovera zvinodhaka kuongororwa kweiyo mycobacterium tuberculosis inovhara 13 zvinodhaka uye gumi nematanhatu eutes sites dzekupokana kwezvinodhaka majini. | Sputum, Alveolar Lavage Fluid, Hydrothorax neAscites, tarisa punctor Sampuli, Cerebropinal Fluid. | Yechipiri / yechitatu chizvarwa zviviri chikuva chepuratifomu |
Pfungwa: HWTS-NG-T001 Mycobacterium Typing uye Dishshish Kudzikisira Kuona Kit (MultiplEx Amplified nzira)
Chigadzirwa Chekutanga
Chigadzirwa chakavakirwa pane chikuru-uye chechipiri-line mishonga inotsanangurwa muTB kurapa kweTB yekurapa, macrodides uye aminoglyconides Uyo wangucobacterium tuberculosis is a my st Kunyanya-kukora kuverenga pamba uye kune imwe nyika.
Typing Kuzivikanwa kunoenderana neNTM Starins Pfupikwi muNTM Nhungamiro Dzakabudiswa neChina Chinyorwa cheTberculosis uye Zvekufema Zvekuenderana Nenyanzvi. Iyo yakagadzirirwa typing primers inogona kukwidza, kuteedzana uye kutsanangurwa zvinopfuura 190 NTM mhando.
Kuburikidza neyakagadziriswa akawanda PCR gency tekinoroji, magrotyping majini ezvinodhaka eMycobageterium yakamisikidzwa naGriplifix pcl, uye iyo amplication musanganiswa wejini emajini kuti aonekwe akawanikwa. Zvigadzirwa zveAmplified zvinogona kuvakwa mune yechipiri-chizvarwa kana chechitatu-yekukwira-kwechipiri-chizvarwa uye chechizvarwa chekusaruramisa mapuranga ekunyora kuti uwane ruzivo rwekuteedzera zvemajini anotarisisa. Nekuenzanisa neyunival scations iri muRefal-muDatabase yeTatabase yeTatabase yeTatabera Yakasanganiswa neyazvinorira sputum sample kurapa mhinduro yeMacro & micro-bvunzo, kakapetwa kakapetwa mitezo yekiriniki) yakagadziriswa, saka kupokana kwezvinodhaka kuongororwa kunogona kuve yakananga yakananga kune kiriniki samples samples.
Chigadzirwa Kuongororwa Range
34zvinodhaka kuramba- zvakabatana majini e18anti-tuberculosis zvinodhaka uye6NTM zvinodhaka zvakaonekwa, chifukidzo297zvinodhaka zvinodhaka nzvimbo; Marudzi gumi eGycobacterium tuberculosis uye anopfuura190Mhando dzeNTM dzakaonekwa.
Tafura 1: Ruzivo rwe 18 + 6 zvinodhaka + 190 + NTM
Kubudirira kwechigadzirwa
Akasimba Clinical Adaptability: Iyo Sputum Samples inogona kuonwirwa zvakananga neako-liquefaction mumiririri pasina tsika.
Iko kushanda kwekuedza kuri nyore: Kutenderera kwekutanga kwekuwedzera kwekushanda kuri nyore, uye yekuvaka raibhurari inopedzwa mumaawa matatu, iyo inovandudza kushanda kwebasa.
Kunyatsotyisidzira typing uye kufukidza kurwisa uye zvinodhaka zvinodhaka zveMT.
Kuenderana: Chigadzirwa kuwirirana, kuchinjika kune mainstream ilm uye mGi / ont mapuratifomu.
Chigadzirwa Chigadzirwa
| Chigadzirwa kodhi | Chigadzirwa Zita | Kuongorora Puratifomu | Zvinyorwa |
| HWTS-NG-T001 | Mycobacterium Typing uye Dishshish Kudzikisira Kuona Kit (MultipLEX AMPLICATION YOKUTI) | Ont, Illumina, Mgi, salus pro | 16 / 96RXN |
Kutumira Nguva: Jan-23-2024