Chombo Chitsva cheTuberculosis Diagnosis uye Kuramba Kwemishonga: A New Generation Targeted Sequencing (tNGS) Yakasanganiswa neMuchina Kudzidza kweTuberculosis Hypersensitivity Diagnosis.
Literature report: CCa: a diagnostic model based on tNGS uye muchina kudzidza, iyo yakakodzera vanhu vane shoma bhakitiriya tuberculosis uye tuberculous meningitis.
Musoro weThesis: Tuberculous-yakanangana nechizvarwa chinotevera kutevedzana uye kudzidza muchina: an Ultra-sensitive diagnostic zano repaucific pulmonary tubulars uye tubular meningitis.
Periodical: 《Clinica Chimica Acta》
IF:6.5
Zuva rekubudiswa: Ndira 2024
Yakasanganiswa neYunivhesiti yeChinese Academy yeScience uye Beijing Chest Hospital yeCapital Medical University, Macro & Micro-Test yakagadzira tuberculosis yekuongororwa modhi yakavakirwa pachizvarwa chitsva cheyakanangwa sequencing (tNGS) tekinoroji uye nzira yekudzidza muchina, iyo yakapa yakanyanya-yakanyanya kuona senitivity yetibhii ine mabhakitiriya mashoma uye tuberculous meningitis, yakapa nzira itsva yehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona hwehutachiona. kuona uye kurapwa kwetibhii. Panguva imwecheteyo, zvinowanikwa kuti plasma yemurwere cfDNA inogona kushandiswa seyakakodzera mhando yemhando yekliniki sampling mukuongororwa kweTBM.
Muchidzidzo ichi, 227 plasma samples uye cerebrospinal fluid samples dzakashandiswa kumisikidza maviri ekiriniki cohorts, umo murabhoritari diagnostic cohort samples dzakashandiswa kumisikidza muchina wekudzidza modhi yechirwere cherurindi, uye kiriniki yekuongorora cohort samples yakashandiswa kuratidza iyo yakagadzwa yekuongororwa modhi. Ese masampuli akatanga kunangana nedziva rakanangwa rakanangwa rekuferefeta reMycobacterium tuberculosis. Zvadaro, zvichibva pane TB-tNGS yekuenzanisa data, muenzaniso wemuti wemusarudzo unoshandiswa kuita 5-fold cross-validation pakudzidziswa uye kusimbiswa kweseti yerabhoritari yekuongorora mutsara, uye mizinga yekuongorora yeplasma samples uye cerebrospinal fluid samples inowanikwa. Chikumbaridzo chakawanikwa chinounzwa mumaseti maviri ebvunzo dzekiriniki yekuongororwa mutsara kuti ionekwe, uye mashandiro ekuongorora emudzidzi anoongororwa neROC curve. Pakupedzisira, nzira yekuongorora yechirwere chetachiona yakawanikwa.
Fig. 1 schematic dhizaini yekutsvagisa dhizaini
Migumisiro: Zvinoenderana nematanho chaiwo eCSF DNA sample (AUC = 0.974) uye plasma cfDNA sampuro (AUC = 0.908) yakatemerwa muchidzidzo ichi, pakati pe227 samples, kunzwisiswa kweCSF sampuro yaive 97.01%, iyo chaiyo yaive 95.65%, uye iyo plasma uye 227 sampleity yaive 227 sampuli. 86.36%. Mukuongorora kwemakumi mana nemana akapetwa samples eplasma cfDNA uye cerebrospinal fluid DNA kubva kuvarwere veTBM, nzira yekuongorora yechidzidzo ichi ine kuenderana kwakanyanya kwe90.91% (40/44) muplasma cfDNA uye cerebrospinal fluid DNA, uye senitivity i95.45% (42/42). Muvana vane pulmonary tuberculosis, chirongwa chekuongorora chechidzidzo ichi chinonyanya kukoshesa sampuli dzeplasma kupfuura Xpert yekuona migumisiro yegastric juice samples kubva kuvarwere vakafanana (28.57% VS 15.38%).
Fig. 2 Ongororo yekushanda kwechirwere chetachiona chetachiona chemuenzaniso wehuwandu hwevanhu
Fig. 3 Diagnostic results of paired samples
Mhedziso: A hypersensitive diagnostic method for tuberculosis yakasimbiswa muchidzidzo ichi, iyo inogona kupa chigadziro chekuongorora nepamusoro-soro yekuona kunzwisiswa kwevarwere vekliniki vane oligobacillary tuberculosis (negative tsika). Kuonekwa kwe hypersensitive tuberculosis yakavakirwa paplasma cfDNA inogona kunge iri mhando yemuenzaniso yakakodzera kuongororwa kwetibhii inoshanda uye tuberculous meningitis (masampuli eplasma ari nyore kuunganidza pane cerebrospinal fluid kune varwere vanofungidzirwa kuti vane chirwere chepfungwa).
Nyaya yekutanga: https://www.sciencedirect.com/science/article/pii/s0009898123004990? kuburikidza ne%3Dihub
Kusuma kupfupi kweMacro & Micro-Test tuberculosis series zvigadzirwa zvekuongorora
Tichitarisa mamiriro ezvinhu akaoma emuenzaniso wevarwere vechirwere chetachiona uye zvido zvakasiyana-siyana, Macro & Micro-Test inopa yakakwana seti yeNGS mhinduro dzekubvisa liquefaction kubva kune sputum samples, Qualcomm raibhurari kuvaka uye kutevedzana, uye kuongororwa kwedata. Zvigadzirwa izvi zvinovhara nekukurumidza kuongororwa kwevarwere vetibhii, kuona zvinodhaka kuramba tibhii, kutaipa mycobacterium tuberculosis uye NTM, hypersensitivity kuongororwa kwebhakitiriya-negative tuberculosis uye vanhu vane tuberculous, nezvimwe.
Seri yekuona kits yetibhii uye mycobacteria:
| Chinhu Nha | zita rechigadzirwa | chigadzirwa chekuongorora zvemukati | mhando yemuenzaniso | muenzaniso unoshanda |
| HWTS-3012 | Muenzaniso wekuburitsa mumiririri | Inoshandiswa mukurapa kwekunyungudika kwemasampuli esputum, yakawana rekodhi rekodhi nhamba, Sutong Machinery Equipment 20230047. | sputum | |
| HWTS-NGS-P00021 | Qualcomm quantity yekuongorora kit ye hypersensitive tuberculosis (probe capture nzira) | Kwete-invasive (liquid biopsy) hypersensitivity yekuona kwebhakitiriya-negative pulmonary tuberculosis uye mazamu europi; Mienzaniso yevanhu vanofungidzirwa kuti vane utachiona hwechirwere chetachiona kana kuti kwete-tuberculosis mycobacteria yakaongororwa nepamusoro-yakadzika sequencing metagenomics, uye ruzivo rwekutsvaga kana chirwere chetachiona chetachiona kana kuti chisingaiti-tuberculosis mycobacteria chaive nehutachiona uye ruzivo rwekutanga rwemishonga yekudzivirira mishonga yemycobacterium tuberculosis yakapiwa. | Peripheral ropa, alveolar lavage fluid, hydrothorax uye ascites, tarisa puncture sample, cerebrospinal fluid. | Chizvarwa chechipiri |
| HWTS-NGS-T001 | Mycobacterium typing uye drug resistance kit (multiplex amplification sequencing nzira) | Mycobacterium typing test, kusanganisira MTBC uye 187 NTM;Kuonekwa kwechirwere cheMycobacterium tuberculosis kunobata mishonga gumi nemitatu uye nzvimbo gumi nenhanhatu dzekuchinja dzemajini ekuramba zvinodhaka. | Sputum, alveolar lavage fluid, hydrothorax uye ascites, tarisa puncture sample, cerebrospinal fluid. | Chechipiri/yechitatu chizvarwa dual platform |
Zvinonyanya kukosha: HWTS-NGS-T001 Mycobacterium kutaipa uye kutaipa kwemishonga yekurwisa zvinodhaka (multiplex amplification nzira)
Product sumo
Chigadzirwa chacho chakavakirwa pamishonga mikuru yekutanga-yechipiri-mutsara inotsanangurwa mu WHO TB nhungamiro yekurapa, macrolides uye aminoglycosides inowanzoshandiswa muNTM nhungamiro yekurapa, uye nzvimbo dzekudzivisa zvinodhaka dzinofukidza boka rimwe chete renzvimbo dzine chekuita nemishonga muWHO Mycobacterium tuberculosis complex mutation catalogue, pamwe chete nezvimwe zvakashumwa zvekuramba zvinodhaka majini uye kuchinjika kwenzvimbo dzekumba uye kuchinjika kwenzvimbo dzekuongorora uye kushanduka kwenzvimbo.
Kuzivikanwa kwetaipa kunobva pamhando dzeNTM dzakapfupikiswa mugwaro reNTM rakaburitswa neChinese Journal yeTuberculosis uye Respiratory Diseases uye kubvumirana kwenyanzvi. Iwo akagadzirirwa kutaipa maprimers anogona kukudza, kutevedzana uye kududzira zvinopfuura zana nemakumi mapfumbamwe emarudzi eNTM.
Kuburikidza nekwakanangwa multiplex PCR amplification tekinoroji, iwo genotyping majini uye geno-resistant genes eMycobacterium akakwidziridzwa nemultiplex PCR, uye musanganiswa weamplicon wemajini anotangwa kuti aonekwe wakawanikwa. Zvigadzirwa zvakakwidziridzwa zvinogona kuvakwa kuita chizvarwa chechipiri kana chechitatu-chizvarwa chepamusoro-chepamusoro-chekutevedza maturakiti, uye ese echizvarwa chechipiri uye chechitatu-chizvarwa chinotevedzana mapuratifomu anogona kuiswa kune yakadzika-yakadzama sequencing kuti uwane ruzivo rwekutevedzana kwemajini anotangwa. Nokuenzanisa neanozivikanwa ekuchinja arimo mune yakavakirwa-mureferensi dhatabhesi (kusanganisira iyo WHO Mycobacterium tuberculosis complex mutation catalog uye hukama hwayo nekupokana nemishonga), shanduko dzine chekuita nekurwisa zvinodhaka kana kubatwa kwemishonga inorwisa tuberculosis yakatemwa. Yakasanganiswa neyakavhurika yega sputum sampuli yekurapa mhinduro yeMacro & Micro-Test, dambudziko rekushomeka kwenyucleic acid amplification yehunyanzvi hwemakiriniki sputum samples (yakapetwa kagumi kupfuura iyo yemaitiro echinyakare) yakagadziriswa, kuitira kuti kurongeka kurongeka kwemishonga kugone kushandiswa zvakananga kumakiriniki esputum samples.
Kuonekwa kwechigadzirwa range
34zvinodhaka kuramba-zvinechekuita majini e18anti-tuberculosis mishonga uye6Mishonga yeNTM yakaonekwa, yakavhara297nzvimbo dzekudzivisa zvinodhaka; Gumi marudzi eMycobacterium tuberculosis uye anopfuura190mhando dzeNTM dzakaonekwa.
Tafura 1: Ruzivo rwe18+6 Mishonga +190+NTM
Product advantage
Yakasimba kuchinjika kwekiriniki: iyo sputum samples inogona kutariswa zvakananga ne-self-liquefaction agent pasina tsika.
Iko kushanda kwekuyedza kuri nyore: denderedzwa rekutanga rekusimudzira kushanda kuri nyore, uye kuvakwa kweraibhurari kunopedzwa mumaawa matatu, izvo zvinovandudza kushanda zvakanaka.
Kutaipa kwakazara uye kuramba zvinodhaka: kuvhara nzvimbo dzekutaipa uye dzekuramba zvinodhaka dzeMTB neNTM, anove mapoinzi akakosha ekiriniki, kutaipa kwakaringana uye kuona kuramba kwezvinodhaka, kutsigira yakazvimirira yekuongorora software, uye kugadzira mishumo yekuongorora nekudzvanya kamwe chete.
Kugarisana: kuenderana kwechigadzirwa, kuchinjika kune makuru ILM uye MGI/ONT mapuratifomu.
Product specification
| Product code | zita rechigadzirwa | Detection platform | specifications |
| HWTS-NGS-T001 | Mycobacterium kutaipa uye zvinodhaka kuramba yekuona kit (multiplex amplification nzira) | ONT, Illumina, MGI, Salus pro | 16/96rxn |
Nguva yekutumira: Jan-23-2024