Fully-Automated Sample-to-Mhinduro C. Diff Infection Detection

Chii chinokonzera C. Diff chirwere?

C.Diff infection inokonzerwa nebhakitiriya rinozivikanwa seClostridioides difficile (C. difficile), iyo inowanzogara isingakuvadzi muura. Zvisinei, kana kuenzana kwemabhakitiriya emudumbu kukanganisika, kazhinji kushandiswa kwemishonga inorwisa mabhakitiriya yakawanda, C. difficile inogona kukura zvakanyanyisa uye kuunza chepfu, zvichiita kuti utachiona.

Utachiona uhu huripo mune zvosetoxicogenicuye kwete-toxigenic mafomu, asi chete toxigenic strains (chepfu A uye B) inokonzera chirwere. Vanotanga kuzvimba nekukanganisa intestinal epithelial masero. Toxin A inonyanya kuita enterotoxin iyo inokuvadza gut lining, kuwedzera permeability, uye kukwezva masero edziviriro anoburitsa cytokines inozvimba. Toxin B, cytotoxin ine simba, inotarisa actin cytoskeleton yemaseru, inotungamira kutenderera kwesero, kuvharika, uye pakupedzisira kufa kwesero. Pamwe chete, chepfu iyi inokonzeresa kukuvadzwa kwematishu uye mhinduro yakasimba yekudzivirira, iyo inoratidzira secolitis, manyoka, uye mumatambudziko akaomarara, pseudomembranous colitis - kuzvimba kwakakomba kwekoloni.

Zvinoita seiC. Diffparadzira?

C.Diff inopararira zviri nyore. Inowanikwa muzvipatara, kazhinji inowanikwa muICUs, pamaoko evashandi vechipatara, pasi pechipatara uye handrails, pamagetsi thermometers, uye zvimwe zvigadzirwa zvekurapa ...

Zvikonzero Zvengozi yeC. Diff Infection

• ●Kugara muchipatara kwenguva refu;

• ●Antimicrobial therapy;

• ●Chemotherapy agents;

• ●Kuvhiyiwa kwemazuva ano (gastric sleeve, gastric bypass, kuvhiyiwa kwekoloni);

• ●Naso-gastric chikafu;

• ●Pamberi C. diff utachiona;

Zviratidzo zveC. Diff infection

C. diff utachiona hunogona kunge husina kugadzikana. Vanhu vazhinji vane manyoka anoenderera mberi uye kusagadzikana mudumbu. Zviratidzo zvinowanzoitika ndezvi: manyoka, kurwadziwa mudumbu, kurutsa, kurasikirwa nechido, fivha.

Sezvo hutachiona hweC. diff huchiwedzera, pachava nekugadzirwa kwechimiro chakaoma zvikuru cheC. diff inozivikanwa se colitis, pseudomembranous enteritis uye kunyange rufu.

KuongororwayeC. Diff Infection

Mabhakitiriya Culture:Sensitive asi nguva inopedza (2-5days), haigoni kusiyanisa toxigenic uye kwete-toxigenic strains;

Tsika dzeToxin:inozivisa toxigenic strains inokonzera chirwere asi inopedza nguva (3-5days) uye isinganyanyi kunzwa;

Kuonekwa kweGDH:kukurumidza (1-2hrs) uye inodhura-inoshanda, inonzwisisa zvakanyanya asi haigone kusiyanisa toxigenic uye isiri-toxigenic strains;

Cell Cytotoxicity Neutralization Assay (CCNA): inoona muchetura A uye B nekunzwa kwakanyanya asi inopedza nguva (2-3days), uye inoda zvivakwa zvehunyanzvi nevashandi vakadzidziswa;

Muchetura A/B ELISA: Nyore uye inokurumidza bvunzo (1-2hrs) ine yakaderera senitivity uye kazhinji nhema dzisina kunaka;

Nucleic Acid Amplification Tests (NAATs): Inokurumidza (1-3hrs) uye yakanyanya kujeka & yakananga, kuona majini ane chekuita nekugadzirwa kwehupfu;

Uyezve, bvunzo dzekufungidzira dzekuongorora ura, dzakadai seCT scans neX-rays, dzinogonawo kushandiswa kubatsira mukuongororwa kweC. diff uye matambudziko eC. diff, akadai secolitis.

Kurapa kweC. Diff utachiona

Nzira dzakawanda dzekurapa dziripoC. diff utachiona. Pazasi pane zvakanakisa sarudzo:

• ●Mishonga yemishonga yemumuromo yakaita sevancomycin, metronidazole kana fidaxomicin ndiyo inonyanya kushandiswa sezvo mushonga unogona kupfuura nemudumbu rekudya uye uchisvika mucolon kunogara C. diff bacteria.

• ●Intravenous metronidazole inogona kushandiswa pakurapa kana C. diff infection yakanyanya.

• ●Fecal microbiota transplants yakaratidza kushanda mukurapa kazhinji C. diff zvirwere uye zvakanyanya C. diff zvirwere zvisingadaviri kumishonga inorwisa mabhakitiriya.

• ●Kuvhiya kungave kuri madikanwa kune zviitiko zvakakomba.

Diagnostic solution kubva kuMMT

Mukupindura kudiwa kwekutsanya, kuona kwakarurama kweC. difficile, tinosuma Nucleic Acid Detection Kit yedu itsva yeClostridium difficile toxin A/B gene, tichipa simba vashandi vehutano kuti vatange uye vanyatsoongorora uye vanotsigira kurwisana nezvirwere zvakawanikwa muchipatara.

图片链接:

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  ●High Sensitivity: Inoona zvakaderera se200 CFU/mL,;

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  ●Kwakarurama Targeting: Inonyatso tsanangura C. difficile toxin A/B gene, kuderedza nhema dzenhema;

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  ●Direct Pathogen Kuonekwa: Inoshandisa nucleic acid yekuyedza kuona zvakananga majini emuchetura, kumisikidza chiyero chegoridhe chekuongorora.

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  ●Inonyatsoenderana nemainstream PCR zviridzwa zvinogadzirisa mamwe marabhu;

Sample-ye-MhinduroMhinduro paMacro & Micro-Test's AIO800 Mobile PCR Lab

 

• ●Sample-ku-Mhinduro otomatiki - Rodha yekutanga sampu machubhu (1.5-12 mL) zvakananga, kubvisa manyore pipetting. Kubvisa, kukwidziridza, uye kuona kwakazara otomatiki, kuderedza maoko-nenguva uye kukanganisa kwevanhu.

• ●Eight-Layer Contamination Dziviriro - Yakananga kuyerera kwemhepo, kudzvanywa kwakashata, HEPA kusefa, UV sterilization, yakavharwa maitiro, uye zvimwe zvakabatanidzwa zvinodzivirira zvinodzivirira vashandi uye kuona mhedzisiro yakavimbika panguva yekuyedzwa kwepamusoro.

Kuti uwane rumwe ruzivo:

https://www.mmtest.com/nucleic-acid-detection-kit-for-clostridium-difficile-toxin-ab-gene-fluorescence-pcr-product/

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