—— Chidzidzo cheKubatsirana chakaitwa neZhejiang CDC, Macro & Micro-Test, neChina CDC Yakaburitswa muFrontiers in Cellular and Infection Microbiology
Pfupiso Yekudzidza
Muna Chivabvu 2026, Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) yakaburitsa bepa rakatungamirwa neZhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), nechikwata che bioinformatics kubva kuBeijing Macro & Micro-Test Bio-Tech Co., Ltd. neNational Institute for Communicable Disease Control and Prevention (China CDC) sevanyori pamwe chete. Chidzidzo ichi chine musoro unoti:
"Kuzivikanwa uye kuongororwa kwephylogenetic kwemarudzi manomwe eBrucella abortus muZhejiang, China."
Chidzidzo ichi chinomiririra ongororo yekutanga yakarongeka, yakavakirwa pakuongorora kutevedzana kweBrucella abortus (B. abortus) muZhejiang Province, China. Chikwata ichi chakaongorora mhando nomwe dzemhuka dzakaunganidzwa kubva muna 2015 kusvika 2025 (mhando ina dzinobva kuvanhu uye nhatu dzinobva kumombe kubva kuJinhua, Quzhou, neNingbo). Zvakawanikwa izvi zvinopa humbowo hwemajini ekwakabva uye nzira dzekutapurirana kwemhando iyi ye "kuchamhembe" munzvimbo isingawanzoitiki yedenda rekumaodzanyemba kumabvazuva kweChina.
Maonero Ekare uye Kukosha Kwazvo
Brucellosis chirwere chinokonzerwa nemhuka chinokonzerwa nemabhakitiriya erudzi rweBrucella. Brucella abortus inonyanya kubata mombe asi inogonawo kukonzera zvirwere kuvanhu. MuChina, brucellosis inoratidza kusiyana kukuru kwenzvimbo: kuwanda kwezvirwere kunoitika mumatunhu ekuchamhembe (semuenzaniso, Inner Mongolia, Shanxi, Heilongjiang). Kusiyana neizvi, matunhu ekumaodzanyemba, kusanganisira Zhejiang, ave achitongwa neBrucella melitensis, nezviitiko zvishoma zveBrucella abortus. Kusiyana uku kwenzvimbo kunoita kuti kutsanangurwa kwemajini uye kuteverwa kweB. abortus muZhejiang kuve chinhu chakakosha pahutano hweveruzhinji.
Nzira uye Zvakawanikwa Zvikuru
Chikwata chekutsvagisa chakatora nzira yakasiyana-siyana inosanganisa molecular biology ne bioinformatics:
1.Kuzivikanwa kwehutachiona uye kunyora kwekutanga
BCSP-31 gene PCR neAMOS-PCR zvakasimbisa kuti ma isolates ese manomwe aive B. abortus.
Multilocus sequence typing (MLST) yakavakirwa pamajini mapfumbamwe ekuchengetedza misha yakaratidza kuti marudzi ese emhuka aingove emhando yeST2, zvichiratidza kufanana kwakanyanya kwemajini pakati pemhando dzeB. abortus dziri kutenderera muZhejiang.
2.Kutsanangurwa kwe genome rese
Kuongororwa kwemajini ese kwakaitwa papuratifomu yeIllumina NovaSeq. Ongororo yeavhareji ye nucleotide identity (ANI) yakaratidza kuti maZhejiang isolates akafanana ne99.99% ne reference strain B. abortus 544.
Ongororo yePan-genome yakaratidza huwandu hwevanhu hwakachengetedzwa zvakanyanya: majini epakati 3,084 akawanikwa, pamwe chete nemajini gumi chete eganda, uye hapana majini epakati kana egore akawanikwa.
3.Majini ekudzivirira utachiona uye mabhakitiriya anodzivirira utachiona
Zvinhu zvinosvika makumi matanhatu nemasere zvine chekuita nehutachiona zvakafanotaurwa, zvichisanganisira nzira dzekare dzakadai seLPS biosynthesis, T4SS secretion system, uye BvrR-BvrS two-component regulatory system. Zvinonyanya kukosha kuti, ese ma isolates akanga asina majini e adhesin bmaA ne btaF. Kuongororwa kwemajini e resistance kwakaona jini re mprF chete muCARD database, pasina zvimwe zvinokonzeresa resistance zvakaonekwa.
4. Kugadziriswazve kwePhylogenetic uye kuteverwa kwekutapurirana kwehutachiona
Ongororo yeCore-genome single-nucleotide polymorphism (cgSNP) yakaisa Zhejiang isolates panzvimbo chaiyo mumuti wephylogenetic wepasi rose. Zvakabuda zvakaratidza kuti mhando dzeZhejiang dzinoumba boka re monophyletic pamwe chete nemhando dzinobva kuRussia, Mongolia, uye matunhu akati wandei ekuchamhembe kweChina (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Boka iri rinopatsanurwazve kuita mapoka matatu akasiyana (Clade 1-3), zvichiratidza zviitiko zvakawanda zvakazvimiririra zvekutangira.
Mhedziso uye Zvazvinoreva
Chidzidzo ichi chinopa data rekutanga reB. abortus rakanyatsojeka mudunhu reZhejiang uye rinopa mhedziso dzinoverengeka dzinokosha:
- Clear nhoroondo yemajini– Mhando dzeB. abortus dziri kutenderera muZhejiang ndedze ST2, dzakachengetedzwa zvakanyanya majini, uye dzinomiririra rudzi rwemombe rwebrucellosis.
2. Evidence yekutumira kwenzvimbo dzakasiyana– Ongororo yephylogenetic haitsigire kuvapo kwedzinza rakazvimirira muZhejiang. Asi, data rinoratidza zvakasimba kuti marudzi aya akabva kuchamhembe kweChina uye anogona kunge akafanana nemarudzi anobva kuRussia neMongolia. Kuvapo kwemapoka matatu epasi kunoreva zviitiko zvakawanda zvakasiyana zvekutangira.
3. Zvinokanganisa hutano hweveruzhinji- Zvakawanikwa izvi zvinoratidza kukosha kwekuongorora chirwere chebrucellosis kuburikidza nejinomu kunyangwe munzvimbo dzisiri dzerudzi urwu dzakadai seZhejiang. Kunyangwe huwandu hwevanhu varipo parizvino hwakaderera, maturusi ekuongorora akasimba akadai secgSNP anogona kunyatsoongorora kwakabva chirwere ichi uye kupa humbowo hwesainzi hwekudzivirira kutapurirana kwezvirwere zvine chekuita nekutakurwa kwezvipfuwo pakati pematunhu.
Basa iri harizadzise chete mukaha wekutsvaga muZhejiang Province asiwo rinopa ruzivo rutsva rwekutanga rwekutarisa utachiona uye kuongororwa kwenjodzi yebrucellosis munharaunda yeYangtze River Delta.
Ruzivo rwePepa:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Kuzivikanwa uye kuongororwa kwephylogenetic kwemarudzi manomwe eBrucella abortus muZhejiang, China. Frontiers in Cellular and Infection Microbiology, 16, 1758965.
Nguva yekutumira: Chikumi-10-2026