Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance
Zita rechigadzirwa
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance Detection Kit (Melting Curve)
Chitupa
CE
Epidemiology
Mycobacterium tuberculosis, iyo inonzi Tubercle bacillus, TB, ndiyo bhakitiriya inokonzera chirwere cherurindi. Parizvino, mishonga yekutanga inoshandiswa zvakanyanya yekurwisa TB inosanganisira isoniazid, rifampicin nehexambutol, nezvimwewo. Mishonga yechipiri yekurwisa TB inosanganisira fluoroquinolones, amikacin nekanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa ndeye linezolid, bedaquiline nedelamani, nezvimwewo. Zvisinei, nekuda kwekushandiswa zvisirizvo kwemishonga yekurwisa TB uye hunhu hwemadziro emasero e mycobacterium tuberculosis, mycobacterium tuberculosis inoita kuti mishonga yekurwisa TB isashande, izvo zvinounza matambudziko makuru mukudzivirira nekurapa TB.
Rifampicin yave ichishandiswa zvakanyanya mukurapa varwere vane TB yemapapu kubvira kuma1970, uye ine mhedzisiro yakakosha. Yave iri sarudzo yekutanga yekupfupisa kurapwa kwechemotherapy kwevarwere vane TB yemapapu. Kusagona kweRifampicin kunonyanya kukonzerwa nekuchinja kwejini reRpoB. Kunyangwe mishonga mitsva yekurwisa TB ichiramba ichibuda, uye kushanda kwevarwere vane TB yemapapu kwakaramba kuchikwira, kuchine kushomeka kwemishonga yekurwisa TB, uye chiitiko chekushandisa mishonga isina musoro mukurapa chakakwira. Zviri pachena kuti Mycobacterium tuberculosis muvarwere vane TB yemapapu haigoni kuurayiwa zvachose nenguva, izvo zvinozopedzisira zvaita kuti murwere asarambe achirwara nemishonga zvakasiyana-siyana, zvichiita kuti chirwere chirambe kwenguva refu, uye zvinowedzera njodzi yekufa kwemurwere.
Chiteshi
| Chiteshi | Nzira uye Mafluorophores | Reaction Buffer A | Reaction Buffer B | Reaction Buffer C |
| Nzira yeFAM | Mutori wenhau: FAM, Quencher: Hapana | rpoB 507-514 | rpoB 513-520 | 38KD uye IS6110 |
| CY5 Channel | Mutori wenhau: CY5, Quencher: Hapana | rpoB 520-527 | rpoB 527-533 | / |
| Mugero weHEX (VIC) | Mutori wenhau: HEX (VIC), Quencher: Hapana | Kudzora kwemukati | Kudzora kwemukati | Kudzora kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ Murima |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Masvoto |
| CV | ≤5.0% |
| LoD | mycobacterium tuberculosis mabhakitiriya 50/mL mhando yemusango inodzivirira rifampicin: 2x103mabhakitiriya/mL homozygous mutant: 2x103mabhakitiriya/mL |
| Kunyatsojeka | Inoona mycobacterium tuberculosis yemusango uye nzvimbo dzekuchinja kwemamwe majini asingapindi mishonga akadai se katG 315G>C\A, InhA-15C> T, mhedzisiro yebvunzo hairatidzi kuramba rifampicin, zvinoreva kuti hapana kubatanidzwa kwemishonga. |
| Zvishandiso Zvinoshanda: | Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeLightCycler480® Yenguva Yechokwadi |
Kufamba Kwebasa
Kana ukashandisa Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) kana Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022-50) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. kuti ubvise tsvina, wedzera 200μL yePositive Control, negative control uye processed sputum sample kuti iongororwe zvakatevedzana, uye wedzera 10μL yeinternal control yoga muPositive Control, negative control uye processed sputum sample kuti iongororwe, uye matanho anotevera anofanira kuitwa zvakanyatsonaka zvichienderana nemirairo yekubvisa tsvina. Sample volume yakabviswa i200μL, uye vhoriyamu yekudzikisa inokurudzirwa i100μL.
