Mycobacterium tuberculosis nucleic acid uye rifumicin kuramba
Chigadzirwa Zita
HWTS-RT074B-Mycobacterium tuberculosis nucleic acid uye rifumicin kuramba kuongororwa kit (kunyungudika curve)
Chitupa
CE
Epidemiology
Mycobacterium tuberculosis, pfupi sebhubhusaiti bacillus, tb, ndiyo mabhakitiriya pathogenic inokonzera kusanganisa. Parizvino, anowanzo shandiswa-mutsara-mutsara anti-tuberculosis zvishuvo zvinosanganisira Isonizid, ethambitolosis, etccin uye etccecurs mishonga, et. . Nekudaro, nekuda kwekushandiswa zvisiri izvo kweanti-tuberculosis zvinodhaka uye hunhu yeiyo cell Wall chimiro cheMycobacterium Tuberculosis, Mycobacterium tuberculosis inovandudza kupokana nedhisheni kune zvinodhaka zvinodhaka, izvo zvinounza zvinetso zvakakomba pakudzivirira uye kurapwa kwechiBerculosis.
Rifampicin yakashandiswa zvakanyanya mukurapa kwekurapa kwePulmonary tuberculosis vevarwere kubva pakupera kwema1970, uye zvine hunhu hwakakosha. Yave iri yekutanga sarudzo yekupfupisa iyo chemotherapy yePulmonary Tuberculosis Varwere. Rifampicin kuramba inonyanya kukonzerwa nekushanduka kweiyo rpob gene gene. Kunyangwe mishonga mitsva yeAnti-tuberculosis inogara ichibuda, uye yekunyanyisa kugona kwePulmonary Tuberculosis Zviripachena, iyo Mycobacterium Tuberculosis Insine Pulmonary tubmelosis haigone kuurayiwa mumatare anodhaka mumuviri wemurwere, anowedzera mukana wechirwere, uye anowedzera njodzi yekufa kwemurwere.
Chiteshi
| Chiteshi | Chiteshi uye fluorophores | Reaction buffer a | Reaction buffer b | Reaction buffer c |
| Fam chiteshi | Mutori wenhau: Fam, Quencher: hapana | RPOB 507-514 | rpob 513-520 | 38kd uye is6110 |
| Cy5 Channel | Mutori wenhau: CY5, Quencher: hapana | RPOB 520-57 | RPOB 527-533 | / |
| Hex (Vic) chiteshi | Mutori wenhau: Hex (Vic), Quencher: hapana | Kutonga kwemukati | Kutonga kwemukati | Kutonga kwemukati |
Technical parameter
| Chengetedza | ≤-18 ℃ Rima |
| Shefu-Hupenyu | Mwedzi gumi nemaviri |
| Type Type | Sputum |
| CV | ≤5.0% |
| Lod | Mycobacterium Tuberculosis 50 Bactia / ML Rifampicin-inodzivirira yemusango mhando: 2x103bactia / ml Homozygous Mutant: 2x103bactia / ml |
| Chokwadi | Inoona mhuka yemusango-tycobacterium tuberculosis uye masisitimu ekurwisa zvinoitwa nemimwe katg 315g> |
| Zviridzwa Zvinoshanda: | Slan-96p chaiyo-nguva pcr system Biorad cfx96 chaiyo-nguva pcr system Lightcycler480® chaiyo-nguva PCR system |
Shanda kuyerera
Kana shandisa macro & micro-test General DNA / rna Kit (HWTS-3019-50, HWTS-3019-48, HWTS-3019-96) (iyo inogona kushandiswa nemacro & micro-test otomatiki Nuclec acid Extractor (HWTS-3006C, HWTS-3006B)) kana macro & micro-test virra / rna Column (HWTS-3022-50) naJiangsu Macro & Micro-Test Med-Tech's Control Controction kudzora kwemukati kwakasiyana mune zvakanaka kudzora, kutonga kwakashata uye kugadzirisa sampula kuti uongororwe, uye matanho akatevera anofanirwa kuitwa zvakanyatsoitwa zvinoenderana nekutsamwa mirairo. Iyo yakabviswa sampuli vhoriyamu ndeye 200μL, uye iyo yakakurudzirwa elution vhoriyamu ndeye 100μl.
