Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance
Product name
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance Detection Kit (Melting Curve)
Chitupa
CE
Epidemiology
Mycobacterium tuberculosis, munguva pfupi seTubercle bacillus, TB, utachiona hwepathogenic hunokonzera tibhii. Parizvino, iyo inowanzo shandiswa yekutanga-anti-tuberculosis mishonga inosanganisira isoniazid, rifampicin uye hexambutol, etc. Mutsara wechipiri anti-tuberculosis mishonga inosanganisira fluoroquinolones, amikacin uye kanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa inonzi linezolid, bedaquiline uye delamani, nezvimwewo, nekuda kwekushandiswa kusina kururama kwemishonga inorwisa tuberculosis uye maitiro e-anti-tuber cell angu. tibhii, mycobacterium tuberculosis inogadzira kusanzwa kwemishonga kumishonga inorwisa tibhii, izvo zvinounza matambudziko akakomba mukudzivirira nekurapa chirwere cherurindi.
Rifampicin yakashandiswa zvakanyanya mukurapa varwere vepulmonary tuberculosis kubva mukupera kwema1970, uye ine simba rakakura. Yakave sarudzo yekutanga kupfupisa chemotherapy yevarwere vepulmonary tuberculosis. Kuramba kweRifampicin kunonyanya kukonzerwa nekushandurwa kwejene rerpoB. Kunyange zvazvo mishonga mitsva yekurwisa tibhii iri kuramba ichibuda, uye kushanda kwekiriniki kwevarwere vepulmonary tuberculosis kwakaramba kuchiwedzera, kuchine kushaikwa kwemishonga inorwisa tibhii, uye chiitiko chekushandisa zvinodhaka zvisina musoro mumakiriniki zvakanyanyisa. Zviri pachena kuti Mycobacterium tuberculosis muvarwere vane pulmonary tuberculosis haigoni kuurayiwa zvachose panguva yakakodzera, iyo inoguma inotungamirira kumadhigirii akasiyana-siyana ekudzivirira zvinodhaka mumuviri wemurwere, inowedzera nguva yechirwere, uye inowedzera ngozi yekufa kwemurwere.
Channel
| Channel | Zviteshi uye Fluorophores | Reaction Buffer A | Reaction Buffer B | Reaction Buffer C |
| FAM Channel | Mutori wenhau: FAM, Quencher: Hapana | rpoB 507-514 | rpoB 513-520 | 38KD uye IS6110 |
| CY5 Channel | Mutori wenhau: CY5, Quencher: Hapana | rpoB 520-527 | rpoB 527-533 | / |
| HEX (VIC) Channel | Mutori wenhau: HEX (VIC), Quencher: Hapana | Kudzora mukati | Kudzora mukati | Kudzora mukati |
Technical Parameters
| Storage | ≤-18℃ Murima |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | Sputum |
| CV | ≤5.0% |
| LoD | mycobacterium tuberculosis 50 mabhakitiriya / mL rifampicin-resistant mhando yemusango: 2x103utachiona/mL homozygous mutant: 2x103utachiona/mL |
| Zvakananga | Inoona mhuka yemusango mycobacterium tuberculosis uye nzvimbo dzekuchinja kwemamwe majini ekuramba zvinodhaka akadai se katG 315G>C\A, InhA-15C> T, mibairo yebvunzo inoratidza kusaramba rifampicin, zvinoreva kuti hapana kuyambuka-reactivity. |
| Zvishandiso Zvinoshanda: | SLAN-96P Chaiyo-Nguva PCR Systems BioRad CFX96 Chaiyo-Nguva PCR System LightCycler480® Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Kana ukashandisa iyo Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HTSW30-60) & Micro-Test Viral DNA/RNA Column (HWTS-3022-50) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd. kuti ibvise, wedzera 200μL yePositive Control, kudzora kusina kunaka uye kushandiswa kwesputum sampu kuti iongororwe mukutevedzana, uye wedzera 10μL mukutonga kwemaitiro asina kunaka, uye wedzera 10μL mukutonga kwemukati. yakaedzwa, uye matanho anotevera anofanira kunyatsoitwa maererano nemirairo yekubvisa. Iyo yakabviswa sampuli vhoriyamu i200μL, uye yakakurudzirwa elution vhoriyamu i100μL.
