Muenzaniso weRelease Release
Zita rechigadzirwa
Chinoburitsa Muenzaniso weMacro & Micro-Test
Chitupa
CE, FDA, NMPA
Zvikamu zvikuru
| Zita | Zvikamu zvikuru | Chikamutsanangudzo | Huwandu |
| Muenzaniso Wekuburitswachinhu chinodzora kupisa | Dithiothreitol, sodium dodecylsulfate (SDS), inhibitor yeRNase,mvura yakachena, inonyungudutsa mvura | 0.5mL/Chirongo | Bhodhoro re50 |
Cherechedza: Zvikamu zviri mumabheti akasiyana-siyana ezvishandiso hazvichinjiki.
Mamiriro ekuchengetedza uye hupenyu hwesherufu
Chengeta uye takura patembiricha yemumba. Nguva yekushanda imwedzi makumi maviri nemana.
Zvishandiso zvinoshanda
Zvishandiso nemidziyo panguva yekugadzirisa sampuro, zvakaita semapipettes, mavortex mixers,mabhati emvura, nezvimwewo.
Zvinodiwa zvemuenzaniso
Machira emumuromo achangobva kuunganidzwa, machira emumhino.
Kunyatsojeka
Kana kit iyi ikashandiswa kubvisa kubva muCV yemukati meimba ine makopi gumi, coefficient of variation (CV, %) yeCt value haipfuuri 10%.
Musiyano wepakati pemabatch
Kana kuratidzwa kwekunyatsojeka kwemukati meimba kuchiedzwa pamabhechi matatu emakiti ari kugadzirwa mukuyedzwa mushure mekubviswa kwadzokororwa uye, coefficient of variation (CV, %) yeCt value haipfuuri 10%.
Kuenzanisa mashandiro
● Kuenzanisa kushanda zvakanaka kwekubvisa
| Kuenzanisa kushanda zvakanaka kwenzira yemagineti uye sample releaser | ||||
| kuisa pfungwa pachinhu chimwe chete | nzira yemagineti yemabhii | muenzaniso wekuburitsa | ||
| orfab | N | orfab | N | |
| 20000 | 28.01 | 28.76 | 28.6 | 29.15 |
| 2000 | 31.53 | 31.9 | 32.35 | 32.37 |
| 500 | 33.8 | 34 | 35.25 | 35.9 |
| 200 | 35.25 | 35.9 | 35.83 | 35.96 |
| 100 | 36.99 | 37.7 | 38.13 | bvisa |
Kubudirira kwekubvisa sample releaser kwakafanana nekwema magnetic beads, uye huwandu hwehutachiona hwaigona kuva 200Copies/mL.
● Kuenzanisa kukosha kweCV
| Kudzokororwa kwekuburitswa kwesampuro yekuburitsa | ||
| kuwanda: 5000Makopi/mL | ORF1ab | N |
| 30.17 | 30.38 | |
| 30.09 | 30.36 | |
| 30.36 | 30.26 | |
| 30.03 | 30.48 | |
| 30.14 | 30.45 | |
| 30.31 | 30.16 | |
| 30.38 | 30.7 | |
| 30.72 | 30.79 | |
| CV | 0.73% | 0.69% |
Pakaedzwa makopi 5,000 /mL, CV ye orFab ne N yaive 0.73% uye 0.69%, zvichiteerana.
