Mhando makumi maviri nemasere dzeHPV Nucleic Acid Detection Kit (Fluorescence PCR)
Zita rechigadzirwa
Mhando makumi maviri nemasere dzeHPV Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Human Papillomavirus (HPV) ndeyemhuri yePapillomaviridae yehutachiona hweDNA hudiki, husina kuvharwa, hwakatenderera kaviri, hune genome hunosvika 8000 base pairs (bp). HPV inotapukira vanhu kuburikidza nekubata zvakananga kana zvisina kunanga zvinhu zvakasvibiswa kana kutapurirana pabonde. Hutachiona uhwu hausi hwemunhu chete, asiwo hune nyama, uye hunogona kutapurira ganda revanhu nemasero emucosal epithelial, zvichikonzera mhando dzakasiyana dzemapillomas kana maronda paganda revanhu uye kukuvara kuri kuwedzera kwe epithelium yekubereka.
Gomarara remumuromo wechibereko ndeimwe yemabundu anonyanya kuipa munhengo dzekubereka dzevakadzi. Zvidzidzo zvakaratidza kuti hutachiona hweHPV hunoramba huripo uye hutachiona hwakawanda ndizvo zvimwe zvezvikonzero zvikuru zvegomarara remumuromo wechibereko. Parizvino, mishonga inoshanda inozivikanwa ichiri kushaikwa yegomarara remumuromo wechibereko rinokonzerwa neHPV, saka kuwanikwa nekukurumidza uye kudzivirira hutachiona hwemumuromo wechibereko hunokonzerwa neHPV ndiyo kiyi yekudzivirira gomarara remumuromo wechibereko. Zvakakosha zvikuru kugadzira bvunzo iri nyore, chaiyo uye inokurumidza yekuongorora chirwere chegomarara remumuromo wechibereko kuti rionekwe uye rirapwe.
Chiteshi
| N/N | Chiteshi | Rudzi |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC(HEX) | Kudzora Kwemukati | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤ -18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Muenzaniso weti, Muenzaniso wetsvina yemuromo wemukadzi, Muenzaniso wetsvina yemukadzi yekubereka |
| CV | <5.0% |
| LoD | Makopi mazana matatu/mL |
| Kunyatsojeka | Hapana kusangana pakati pezvirwere zvine chekuita nenhengo dzekubereka uye DNA yemunhu. |
| Zvishandiso Zvinoshanda | Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
Kufamba kwebasa: Ona Kit IFU kuti uwane rumwe ruzivo
Mhinduro 1 (Kubviswa kwe nucleic acid otomatiki)
Mhinduro 2: (Macro & Micro-Test Sample Release Reagent)
Ma reagents anoda asi haana kupihwa
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Zvinoshandiswa zvinodiwa asi hazvipihwe
1.5mL yemachubhu asina DNase/RNase, matipi asina DNase/RNase, 8-tube strips yePCR, tabletop centrifuge, tabletop shaker mixer.
