Kuchinja kwemajini ePML-RARA eHuman
Zita rechigadzirwa
HWTS-TM017AKiti yeKuona Kuchinja Kwemajini eHuman PML-RARA (Fluorescence PCR)
Epidemiology
Acute promyelocytic leukemia (APL) imhando yakasarudzika yeacute myeloid leukemia (AML). Vanenge 95% yevarwere veAPL vanoperekedzwa neshanduko yakakosha yecytogenetic, inova t(15;17)(q22;q21), iyo inoita kuti gene rePML riri pachromosome 15 uye retinoic acid receptor α gene (RARA) riri pachromosome 17 risanganiswe kuti rigadzire gene rePML-RARA fusion. Nekuda kwenzvimbo dzakasiyana dzePML gene, gene rePML-RARA fusion rinogona kukamurwa kuita long type (L type), short type (S type) uye variant type (V type), zvichiita kuti rive ne55%, 40% uye 5% zvichiteerana.
Chiteshi
| FAM | Jini rekubatanidza rePML-RARA |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ Murima |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | mwongo wemapfupa |
| CV | <5.0% |
| LoD | Makopi 1000/mL. |
| Kunyatsojeka | Hapana kusangana kwemajini ekubatanidza majini eBCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, uye TEL-AML1. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinhu chinokurudzirwa chekubvisa RNAprep Pure Blood Total RNA Extraction Kit (DP433). Kubvisa RNA kunofanirwa kuitwa zvichienderana neIFU.
