Hunhu Cytomegalovus (HCMV) Nucleic acid
Chigadzirwa Zita
HWTS-UR008A-Human Cytomegalovus (HCMV) Nucleic acid Kuona Kit (Fluorescence PCR)
Epidemiology
Human Cytomegalovirus (HCMV) inhengo ine geniome yakakura mumhuri yemitambo uye inogona kuendesa zvinopfuura mazana maviri mapuroteni. HCMV yakadzikiswa zvakarambadzirwa mumhando yayo yekutambira kuvanhu, uye kuchine mhando yemhuka yehutachiona hwayo. HCMV ine nguva inononoka uye yakareba yekudzokorora kuti iite intranuclear yekubatanidza muviri, uye inokonzeresa kugadzirwa kwePerinuClear uye Cytoplasmic Inclusion yemitumbi uye masero ekuzvimba (makuru masero), saka maseru. Sekureva kwehupfuga kweiyo genome uye phenotype, hcnotype, hcmv inogona kukonzerwa nemhando dzakasiyana dzemarara, pakati pezvimwe zvakasiyana, zvakadaro, hazviratidzi kukosha kwekiriniki.
Hcmv utachiona husina hurongwa, ndezvipi zviratidzo zvakawanda, zvine zviratidzo zvakaoma uye zvakasiyana-siyana, uye zvinogona kuita kuti varwere vevaterite. Purpura. HCMV utachiona hunowanzoitika uye zvinoita sekuparadzira pasi rese. Iyo inopararira zvakanyanya muhuwandu, ine zviitiko zvemitambo makumi mana-50% uye anopfuura 90% munyika dzakakudziridzwa uye dzichiri kusimukira, zvichiteerana. HCMV inogona kubhadhara dormant mumuviri kwenguva yakareba. Kana kushandisirwa muviri muviri kuchinge kwashaiwa simba, hutachiona huchavigirwa kuti zvikonzere hosha, kunyanya hutachiona hwevarwere uye hunogona kukonzeresa varwere necrosis uye kuisa pangozi hupenyu hwevarwere mumatanho akanyanya. Kuwedzera kune kuchata, kutakura zvisizvo uye kusarongeka kwekutapukira kuburikidza nekutapukira
Chiteshi
Nhamo | Hcmv dna |
Vic (Hex) | Kutonga kwemukati |
Technical parameter
Chengetedza | Mvura: ≤-18 ℃ murima |
Shefu-Hupenyu | Mwedzi gumi nemaviri |
Type Type | Serum sampuli, plasma samples |
Ct | ≤38 |
CV | ≤5.0% |
Lod | 50 makopi / maitiro |
Chokwadi | Iko hakuna kuyambuka neHepatitis B turus, Hepatitis Curus, Heppes Virus, Herpes Simplex Virus Type 1, Herpes Simplex Virus Type 2, akajairika samples yeVanhu, nezvimwe. |
Zviridzwa Zvinoshanda: | Inogona kufananidza iyo huru yeFluorescent PCR zviridzwa pamusika. Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva pcr system Lightcycler ®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRR Kuongororwa system Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (HWTS-3006C, HWTS-3006B)) Na Jiangsu Macro & Micro-Test Med-Tech Co, Ltd .. Iyo yekubvisa inofanira kubviswa zvinoenderana nemirairo. Iyo yekuwedzera sampuli vhoriyamu ndeye 200μl, uye iyo yakakurudzirwa elution vhoriyamu ndeye 80μl.
Yakakurudzirwa Reagent Reagent: QiaAPAM DNA Mini Kit (51304), Nucleic acid yekubvisa kana kuchenesa Reagent (YDP315) naTianen Biotech (Beijing) Co, LTD. inofanirwa kubviswa maererano nemirayiridzo yekubvisa, uye iyo yakakurudzirwa vhoriyamu ndeye 200 μL uye iyo yakakurudzirwa vhoriyamu yeElution ndeye 100 μl.