Mhando Sere dzeMavhairasi Ekufema
Zita rechigadzirwa
HWTS-RT184-Mhando Sere dzeMavhairasi Ekufema Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Utachiona hwemunzira yekufema ndiyo mhando yechirwere chinowanzoitika kuvanhu, chinogona kuitika chero muvanhurume kana muvanhukadzi, zera uye nzvimbo, uye chimwe chezvikonzero zvikuru zvekurwara nerufu pasi rose.[1]. Zvirwere zvekufema zvinowanzoonekwa mukurapa zvinosanganisira vhairasi yefuruwenza A (IFV A), vhairasi yefuruwenza B (IFV B), vhairasi yekufema inopindirana netsinga, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) uye mycoplasma pneumoniae, nezvimwewo.[2,3]Zviratidzo zvekiriniki zvinokonzerwa nehutachiona hwemunzira yekufema zvakafanana, asi hutachiona hunokonzerwa nehutachiona hwakasiyana hune nzira dzakasiyana dzekurapa, mhedzisiro yekurapa uye mafambiro echirwere.[4,5]Parizvino, nzira huru dzekuongorora utachiona hwekufema murabhoritari dzinosanganisira: kupatsanurwa kwehutachiona, kuonekwa kweantigen uye kuonekwa kwe nucleic acid. Kit iyi inoona uye inoziva mavhairasi chaiwo e nucleic acids muvanhu vane zviratidzo zvehutachiona hwekufema, pamwe chete nezvimwe mhedzisiro yekiriniki neyerabhoritari kubatsira mukuongorora hutachiona hwekufema.
Magadzirirwo ehunyanzvi
| Kuchengetera | 2-8℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | swab yemumuromo; swab yemumhino |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | Makopi mazana maviri/mL |
| Kunyatsojeka | Kupindirana kwemasero: Hapana kuwirirana pakati pekiti nehutachiona hweBoca, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, human genomic DNA. Kuongororwa kwemushonga: Sarudza mucin (60mg/mL), ropa revanhu (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride ine 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), Zvakabuda zvakaratidza kuti zvinhu zvinokanganisa zviri pamusoro apa |
| Zvishandiso Zvinoshanda | Inoshanda kune type I detection reagent: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Masisitimu ePCR Enguva Yechokwadi, SLAN-96P Masisitimu ePCR Enguva Yechokwadi (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Masisitimu Ekuona PCR Enguva Yechokwadi (FQD-96A, tekinoroji yeHangzhou Bioer), MA-6000 Muchina Wekutenderera Unopisa Wenguva Yechokwadi (Suzhou Molarray Co., Ltd.), BioRad CFX96 Sisitimu yePCR Yenguva Yechokwadi, BioRad CFX Opus 96 Sisitimu yePCR Yenguva Yechokwadi. Inoshanda kune reagent yekuona mhando yechipiri: EudemonTM AIO800 (HWTS-EQ007) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kufamba Kwebasa
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), uye Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (inogona kushandiswa neEudemonTM AIO800 (HWTS-EQ007)) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Vhoriyamu yemuenzaniso yakatorwa i200μL uye vhoriyamu yekudzikisa inokurudzirwa i150μL.
