Chlamydia Trachomatis, Neisseria Gonorrhoeae uye Trichomonas vaginalis
Zita rechigadzirwa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae uye Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chlamydia trachomatis (CT) rudzi rwehutachiona hweprokaryotic hunokanganisa masero eukaryotic chete. Chlamydia trachomatis yakakamurwa kuita maAK serotypes zvichienderana nenzira ye serotype. Utachiona hwemumaronda emudumbu hunowanzo kukonzerwa ne trachoma biological variant DK serotypes, uye varume vanowanzoonekwa se urethritis, iyo inogona kurapwa pasina kurapwa, asi yakawanda yacho inova isingaperi, inowedzerwa nguva nenguva, uye inogona kusanganiswa ne epididymitis, proctitis, nezvimwewo.
Chiteshi
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | -18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Chipfuko chemuromo wechibereko chemukadzi,Chidzikiso chekuisa mushonga wevakadzi,Chinobvisa urethra chemurume |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Makopi/mL |
| Kunyatsojeka | Hapana kusangana kwezvirwere zveSTD kunze kwenzvimbo yekuongorora, zvakaita seTreponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, nezvimwewo. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Reagent inokurudzirwa yekuburitsa: Pipette 1mL yemuenzaniso inofanira kuyedzwa kusvika pa 1.5mL yechubhu yeDNase/RNase-free centrifuge, centrifuge pa 12000rpm kwemaminitsi matatu, rasa supernatant wochengeta precipitate. Wedzera 200µL yemunyu wakajairika kune precipitate kuti udzokezve. Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanirwa kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakabviswa i200µL, uye vhoriyamu yekudzikisa inokurudzirwa i80µL.
Sarudzo yechipiri.
Chinhu chinokurudzirwa chekubvisa: Nucleic Acid Extraction kana Purification Kit (YDP302). Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nemirairo yekushandisa. Vhoriyamu yekubvisa inokurudzirwa i80µL.
