Gene Resistance yeCarbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Zita rechigadzirwa
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Detection Kit (Fluorescence PCR)
Epidemiology
Mishonga inorwisa mabhakitiriya yeCarbapenem mishonga inorwisa mabhakitiriya yeβ-lactam isingawanzoitiki ine hutachiona hwakakura uye ine simba guru rekurwisa mabhakitiriya. Nekuda kwekugadzikana kwayo kuβ-lactamase uye chepfu shoma, yave imwe yemishonga inonyanya kukosha yekurapa hutachiona hwakasimba hwebhakitiriya. Carbapenems yakasimba zvikuru kuplasmid-mediated extended-spectrum β-lactamases (ESBLs), machromosomes, uye plasmid-mediated cephalosporinases (AmpC enzymes).
Chiteshi
| PCR-Musanganiswa 1 | PCR-Musanganiswa 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Kudzora Kwemukati | Kudzora Kwemukati |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Makonye, makonye akachena, swab yemudumbu |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Kunyatsojeka | a) Kit iyi inoona mareferensi asina kunaka ekambani, uye mhedzisiro yacho inosangana nezvinodiwa nemareferensi anoenderana. b) Mhedzisiro yebvunzo ye cross-reactivity inoratidza kuti kit iyi haina cross reaction nezvimwe zvirwere zvekufema, zvakaita seKlebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, kana samples dzine mamwe majini asingagamuchirwe nemishonga CTX, mecA, SME, SHV, TEM, nezvimwewo. c) Zvinodzivirira kukanganiswa: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin zvinosarudzwa kuti zviongororwe kukanganiswa, uye mhedzisiro inoratidza kuti zvinhu zvinokanganisa zvataurwa pamusoro apa hazvina maitiro ekuvhiringidza pakuonekwa kwemajini ekudzivirira carbapenem KPC, NDM, OXA48, OXA23, VIM, uye IMP. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P eNguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A，HangzhouTekinoroji yeBioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Chinorayirwa kubvisa chinhu: Macro & Micro-Test General DNA/RNA Kit (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Wedzera 200μL yemunyu wakajairika kune thallus precipitate. Matanho anotevera anofanira kutevedzera mirairo yekubvisa, uye huwandu hweelution hunokurudzirwa ndehwekuti100μL.
Sarudzo yechipiri.
Reagent inokurudzirwa yekuburitsa: Nucleic Acid Extraction kana Purification Reagent (YDP302) naTiangen Biotech (Beijing) Co., Ltd. Kubvisa kunofanira kutanga zvichitevedza danho rechipiri remirairo yekushandisa (wedzera 200μL yebuffer GA kune thallus precipitate, wozunza kusvika thallus yamira zvachose). Shandisa mvura isina RNase/DNase kuti ubvise, uye huwandu hwe elution hwakakurudzirwa i100μL.
Sarudzo 3.
Chinobviswa chinokurudzirwa: Chinobviswa masero emvura neMicro-Test Sample Release Reagent. Sample yesputum inofanira kusukwa nekuwedzera 1mL yemunyu wakajairika kune thallus precipitate yataurwa pamusoro apa, ichiiswa centrifuge pa 13000r/min kwemaminitsi mashanu, uye supernatant inoraswa (chengeta 10-20µL yesupernatant). Kuti uwane colony yakachena uye rectal swab, wedzera 50μL yesample release reagent zvakananga kune thallus precipitate yataurwa pamusoro apa, uye matanho anotevera anofanira kutorwa zvichienderana nemirairo yekushandisa.
