Carbenem Ristance Gene (KPC / NdM / Oxa 48 / Oxa 23 / Vim / Imp)
Chigadzirwa Zita
HWTS-OT045 Carbenem Inodziya Gene (KPC / NdM / Oxa 48 / Oxa 23 / Vim / Import) Kuona Kit (Fluorescence PCR)
Epidemiology
Carbenem mishonga inorwisa mishonga yeAtypical β-lctam mishonga yemiindarakatoro neyekupedzisira antibacterial spectrum uye yakasimba antibacterial basa. Nekuda kwekugadzikana kwavo ku β-Lactamase uye yakaderera yehupfu, iyo yave imwe yeanokosha antibacterial mishonga yekurapa kwehutachiona hwakanyanya hutachiona hwehutachiona hwehutachiona. Carbenems akanyanya kugadzikana kusvika plasmid-yakadungamidzana yakawedzerwa-spectrum β-esbls), chromosomes, uye plasmes-yakanunura cephaloporuses (ampc enzymes).
Chiteshi
| PCR-Sanganisa 1 | PCR-Sanganisa 2 | |
| Nhamo | Imp | Vim |
| Vic / Hex | Kutonga kwemukati | Kutonga kwemukati |
| CY5 | Ndim | KPC |
| Rox | Oxa48
| Oxa23 |
Technical parameter
| Chengetedza | ≤-18 ℃ |
| Shefu-Hupenyu | Mwedzi gumi nemaviri |
| Type Type | Sputum, yakachena colonies, rectal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lod | 103CFU / ML |
| Chokwadi | a) Iyo kit inoona iyo yakagadziriswa kambani yakaipa mareferensi mareferensi, uye mhedzisiro yacho inosangana nezvinodiwa zvezvinhu zvinoenderana. b) Mhedzisiro yeyekuyambuka-tarisiro inoratidza kuti kit iyi isina maitiro ezvimwe zvinofema, Haemophilus Alemenzae, Acinosgetter Meca, SME, SHV, Tem, nezvimwe. c kuongororwa Carbenem Kurwisa majini KPC, NdM, Oxa48, Oxa23, Vim, uye Imp. |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva PCR system (Hongshi Medical Technology Co, Ltd.) Lightcycler ®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PCR Kuongororwa system (FQD-96A,HangzhouBioer tekinoroji) Ma-6000 chaiyo-nguva quolitative thermal cyler (Suzhou molarray co., Ltd.) Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Sarudzo 1.
Yakakurudzirwa Regency Reagent: Macro & Micro-Test General DNA / rna Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019- Iyo Thallus inoyera. Matanho akatevera matanho anofanirwa kutevera iyo mirairo yekubvisa, uye iyo yakakurudzirwa elution vhoriyamu ndeye100μl.
Sarudzo 2.
Yakakurudzirwa Reagent Reagent: Nuclec Acid Exction , uye kuzunungusa kusvikira thallus yakamiswa zvizere). Shandisa rnase / dnase yemvura yemahara yekukura, uye iyo ecution elution vhoriyamu ndeye 100μl.
Sarudzo 3.
Yakakurudzirwa Yakagadziriswa Reagent: Macro & micro-bvunzo sampula sununguko reagent. Sampula sampuli inoda kushambidzwa nekuwedzera 1ml yeyakajairwa saline kune iri pamusoro apa BASATE BEATHATE, Min kwemaminitsi mashanu, uye supernatt Kune yakachena koloni uye rectal Swab, wedzera 50μL ye sampula sumpla yakananga kune iyo yakataurwa pamusoro apa Thallus inodonhedza, uye matanho akatevera anofanira kubviswa maererano nemirairo yekushandiswa.
