Kiti yeKuona Nucleic Acid yakavakirwa paEnzymatic Probe Isothermal Amplification (EPIA) yeCandida Albicans
Zita rechigadzirwa
Kiti yeKuona Nucleic Acid yakavakirwa paEnzymatic Probe Isothermal Amplification (EPIA) yeCandida Albicans
Chitupa
CE
Epidemiology
Rudzi rweCandida ndiyo mhando yefungus yakakura kwazvo mumuviri wemunhu, inowanikwa zvakanyanya mumitsipa yekufema, mudumbu, mumitsipa yegenitourinary nedzimwe nhengo dzinotaurirana nenyika yekunze. Haisi hutachiona hunowanzo konzera zvirwere uye inokonzerwa nemabhakitiriya anokonzera zvirwere. Nekuda kwekushandiswa kwakanyanya kwemishonga inodzvinyirira immune, kugadzirwa kweradiotherapy yebundu, chemotherapy, kurapwa kwemukati, uye kuiswa kwenhengo, uye kushandiswa kwakapararira kwemishonga yakawanda inorwisa mabhakitiriya, maruva akajairika anova asina kuenzana, zvichikonzera hutachiona hweCandida mumitsipa yegenitourinary nemidzipa yekufema.
Utachiona hweCandida munhengo dzesikarudzi dzepabonde hunogona kukonzera vakadzi kurwara nechirwere checandidiasis uye vaginitis, uye hunokonzera varume kurwara nechirwere checandidiasis, acroposthitis uye prostatitis, izvo zvinokanganisa zvakanyanya hupenyu nebasa revarwere. Huwandu hwecandidiasis yenhengo dzesikarudzi huri kuwedzera gore negore. Pakati pazvo, hutachiona hwecandidiasis yenhengo dzesikarudzi dzevakadzi hunosvika 36%, uye varume vanosvika 9%, uye hutachiona hweCandida albicans (CA) ndihwo hukuru, hunosvika 80%.
Utachiona hwefungus hunowanzoonekwa nehutachiona hweCandida albicans ndicho chikonzero chikuru cherufu nekuda kwehutachiona hwepanzvimbo dzinoonekwa vanhu vakawanda. Pakati pevarwere vanonyanya kurwara vari muICU, hutachiona hweCandida albicans hunosvika 40%. Pakati pehutachiona hwefungus hwemudumbu, hutachiona hwefungus hwemumapapu ndihwo hunonyanya kuwanda uye huri kuwedzera gore negore. Kuongororwa nekukurumidza uye kuzivikanwa kwehutachiona hwefungus hwemumapapu kune kukosha kwakakosha mukurapa.
Mishumo yekiriniki iripo yeCandida albicans genotypes inonyanya kusanganisira mhando A, mhando B, uye mhando C, uye mhando nhatu dzakadaro dzinopfuura 90%. Kuongororwa kwakarurama kwehutachiona hweCandida albicans kunogona kupa humbowo hwekuongororwa nekurapwa kwe candidiasis vulvitis uye vaginitis, candidiasis yevarume, acroposthitis uye prostatitis, uye hutachiona hweCandida albicans hwemunzira yekufema.
Chiteshi
| FAM | asidhi yeCA nucleic |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ Murima; Yakapfava: ≤30℃ Murima |
| Nguva yekuzorora | Mvura: mwedzi 9; Lyophilized: mwedzi 12 |
| Rudzi rwemuenzaniso | Chinotsvaira Nzira Yemukati Menhengo Dzepabonde, Chipunu |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Makopi mashanu/µL, mabhakitiriya 102/mL |
| Kunyatsojeka | Hapana kusangana kwezvirwere zve genitourinary tract infections, zvakaita seCandida tropicalis, Candida glabrata, Trichomonas vaginalis, Chlamydia trachomatis, Ureaplasma urealyticum, Neisseria gonorrhoeae, Group B streptococcus, Herpes simplex virus type 2, nezvimwewo; hapana kusangana kwezvirwere zve immune system pakati pe kit iyi nezvimwe zvirwere zve immune system, zvakaita seAdenovirus, Mycobacterium tuberculosis, Klebsiella pneumoniae, Gwirikwiti, Candida tropicalis, Candida glabrata uye ma sputum samples evanhu, nezvimwewo. |
| Zvishandiso Zvinoshanda | Sisitimu Yekuona Isothermal yeFluorescence iri Nyore (HWTS1600) Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Zvinodiwa zvemaReagent asi hazvipihwe
Munyu wakajairika, 4% NaOH uye Macro & Micro-Test Sputum Digestion Solution (HWTS-3012), Lysozyme (RT401) yakagadzirwa neTiangen Biotech (Beijing) Co., Ltd., lysozyme diluent (20mM Tris, pH 8.0; 2mM Na2-EDTA; 1.2% Triton), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006), Nucleic Acid Extraction kana Purification Kit (YDP302) yakagadzirwa neTiangen Biotech (Beijing) Co., Ltd.
Zvinoshandiswa zvinodiwa asi hazvipihwe
1.5mL yechubhu yeRNase/DNase isina centrifuge, RNase/DNase-free Tips, benchtop centrifuge, benchtop shakening mixer, uye magnetic stand.
