Borrelia Burgdorferi Nucleic Acid
Zita rechigadzirwa
HWTS-OT076 Borrelia Burgdorferi Nucleic Acid Detection Kit (Fluorescence PCR)
Chitupa
CE
Epidemiology
Chirwere cheLyme chinokonzerwa nehutachiona hweBorrelia burgdorferi uye chinonyanya kutapuriranwa pakati pemhuka dzinogara mhuka, pakati pemhuka dzinogara mhuka nevanhu nezvipembenene zvakaoma. Borrelia burgdorferi inogona kukonzera Erythema chronicum migrans kuvanhu, pamwe nezvirwere zvinosanganisira masisitimu akasiyana-siyana akadai semwoyo, tsinga, nemajoini, nezvimwewo, uye zviratidzo zvekiriniki zvakasiyana-siyana. Zvichienderana nekukura kwezvirwere, zvinogona kukamurwa kuita hutachiona hwepakutanga, hutachiona hwakapararira pakati uye hutachiona hunoramba huripo kwenguva refu, izvo zvinokuvadza hutano hwevanhu. Nokudaro, mukuongororwa kwekiriniki kweBorrelia burgdorferi, zvakakosha zvikuru kugadzira nzira iri nyore, chaiyo uye inokurumidza yekuongorora Borrelia burgdorferi.
Chiteshi
| FAM | DNA yeBorrelia burgdorferi |
| VIC/HEX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Ropa rose |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/mL |
| Zvishandiso Zvinoshanda | Masisitimu eABI 7500 PCR Enguva Yechokwadi Masisitimu ePCR eABI 7500 Anokurumidza Kushanda Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Kiti yeMidi yeropa yeQIAamp DNA Blood Midi Kit yakagadzirwa naQiagen (51185).It inofanira kuburitswazvichienderana nemutemokune mirairo, uye vhoriyamu yekudzikisa inokurudzirwa ndeye100μL.
Sarudzo yechipiri.
RopaGDNA ye enomicEKiti yekudhonza (DP318,Aihwa.: JingchangRekodhi yemudziyo20210062) yakagadzirwa neTiangen Biochemical Technology (Beijing) Co., Ltd.. It inofanira kuburitswazvichienderana nemutemokune mirairo, uye vhoriyamu yekudzikisa inokurudzirwa ndeye100μL.
Sarudzo 3.
Kiti yekuchenesa DNA yeWizard® Genomic (A1120) yakagadzirwa nePromega.It inofanira kuburitswazvichienderana nemutemokune mirairo, uye vhoriyamu yekudzikisa inokurudzirwa ndeye100μL.
