Mhando ina dzehutachiona hwekufema
Zita rechigadzirwa
HWTS-RT099- Mhando 4 dzeMavhairasi Ekufema Nucleic Acid Detection Kit (Fluorescence PCR)-NED-ABI 7500 Real-Time PCR System/ABI 7500 Masisitimu ePCR Anokurumidza Munguva Yechokwadi/QuantStudio®Masisitimu mashanu ePCR enguva chaiyo
HWTS-RT158-4 Mhando dzeMavhairasi Ekufema Nucleic Acid Detection Kit (Fluorescence PCR)-内参Quasar 705
Epidemiology
Chirwere cheCoronavirus 2019, chinonzi "COVID-19", chinoreva pneumonia inokonzerwa ne2019-nCoVhutachiona.2019-nCoVchirwere chekoronavirus chiri murudzi rweβ. COVID-19 chirwere chinotapukira chinopararira nekukurumidza, uye vanhu vanowanzo kubatwa nechirwere ichi. Parizvino, hutachiona hunonyanya kukonzerwa nevarwere vane2019-nCoV, uye vanhu vane hutachiona husina zviratidzo vanogonawo kuva chikonzero chehutachiona. Zvichienderana nekuferefeta kuri kuitwa pari zvino kwezvirwere, nguva yekuzvitakura imazuva 1-14, kazhinji mazuva 3-7. Fivha, kukosora kwakaoma uye kuneta ndizvo zviratidzo zvikuru. Varwere vashoma vaive nezviratidzo.zvakaita semhino dzakazadzwa, mhino dzinoyerera, pahuro panorwadza, myalgia uye manyoka, nezvimwewo.
Chiteshi
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | -18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Chidzitiro chemuromo |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Makopi/mLVirusi refuruwenza A/vhairasi refuruwenza B/vhairasi rekufema risingawirirani: 500Kopi/mL |
| Kunyatsojeka | a) Mhedzisiro yekubatana inoratidza kuti hapana kusangana pakati pekiti ne coronavirus yevanhu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid. b) Kugona kudzivirira kukanganiswa: mucin yakasiyana (60mg/mL), 10% (v/v) yeropa uye phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (kusanganisira zvinochengetedza) (20 mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) uye tobramycin (0.6mg/mL) kuti zviongororwe kupindirana kwemishonga, uye mhedzisiro yacho inoratidza kuti zvinhu zvinopindirana nehuwandu hwataurwa pamusoro apa hazvina kupindirana kwemishonga kumhedzisiro yekuedzwa kwezvirwere. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-TimeMaitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Vhoriyamu yemuenzaniso yakatorwa i200μL, uye vhoriyamu yekudzikisa inokurudzirwa i80μL.
Sarudzo yechipiri.
QIAamp Viral RNA Mini Kit (52904) yakagadzirwa neQIAGEN kana Nucleic Acid Extraction kana Purification Kit (YDP315-R) yakagadzirwa neTiangen Biotech (Beijing) Co., Ltd. Vhoriyamu yemuenzaniso yakatorwa i140μL, uye vhoriyamu yekudzikisa inokurudzirwa i60μL.
