17 Mhando dzeHPV (16/18/6/11/44 Typing)
Chigadzirwa Zita
HWTS-CC015 17 Mhando dzeMunhu Papillomavirus (16/18/1/11/44 Typing) Nucleic Acid Kuona Kit (Fluorescence PCR)
Epidemiology
Cervical cancer ndeimwe yeanonyanya kutyisa tumors mudura rekubereka. Izvo zvakaratidzwa kuti HPV yeHPV yehutachiona uye hutachiona hwakawanda nderimwe rezvikonzero zvikuru zvekanza yechibereko. Parizvino kuchine kushomeka kwekurapwa kwakanyatsogamuchirwa kwechibereko chechibereko chakakonzerwa neHPV. Naizvozvo, zvekutanga kuona uye kudzivirira kwechibereko zvakakonzerwa neHPV ndiwo makiyi ekudzivirira cancer cancer. Kugadzwa kweyekuyevedza, kwakananga uye nekukurumidza uye nekukurumidza zviongorori zvechitendero kune kukosha kukuru kwekuongororwa kwekiriniki kwechirume chekanza.
Chiteshi
PCR-Mix1 | Nhamo | 18 |
Vic / Hex | 16 | |
Rox | 31,33,35,35,45,55,52,56,5,5,59,66,68 | |
CY5 | Kutonga kwemukati |
PCR -MiX2 | Nhamo | 6 |
Vic / Hex | 11 | |
Rox | 44 | |
CY5 | Kutonga kwemukati |
Technical parameter
Chengetedza | -18 ℃ |
Shefu-Hupenyu | Mwedzi gumi nemaviri |
Type Type | Urini sampuli, vakadzi cervical swab sampuli, vakadzi vaginal swab sampula |
Ct | ≤28 |
Lod | 300copiji / ml |
Chokwadi | Iko hakuna kuyambuka-kuita pamwe neUreaplasma Urealictics yeTurakiti Rekubereka, Candidalia Gonorrhoeae, Trichorasas Vaginalis, Mold, Gardnerla uye mamwe marudzi eHPV asina kuvharwa nekitu. |
Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Slan-96p chaiyo-nguva pcr system Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRC Kuonekwa Systems Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Yakakurudzirwa Reagent Reagent: Macro & Micro & Micro-Test Virara / rna Kit (HWTS-3017-50, HWTS-3017-98) (iyo inogona kushandiswa nemacro & micro-bvunzo Otomatiki Acleic acid Extractor (HWTS-3006C, HWTS-3006B)) NaJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Wedzera 200μL yeyakajairika saline kuti udzorere pellet in the stepting Iyo Yakakurudzirwa Elution Vhoriyamu ndeye 80μL.
Yakakurudzirwa Reagent Reagent: QiaApp DNA Mini Kit (51304) kana macro & micro-test virra / rna column (hwts-3020-50). Wedzera 200μL yeyakajairwa saline kuti uendese pellet mudanho 2.1, uyezve kuongororwa kunofanirwa kuitiswa maererano nemirairo yekushandisa iyi yekuwedzera Iyo yakabviswa sampuli vhoriyamu yemasampuli ese mazana maviri nemakumi mairi 200μl, uye iyo yakakurudzirwa vhoriyamu vhoriyamu ndeye 100μl.
Yakakurudzirwa Reagent Reagent: Macro & Micro & Micro-Test Sampula Sunungura Reagent (HWTS-3005-8i, Hwts-3005-8k, HWTS-HWTS-HWTS-HWTS-HWTS-HWTS-HWTS-HWTS-HWTS Wedzera 200μL yeSampu Sununguko Regent Regedza iyo Pellet Indira muNhanho