Mhando gumi nenomwe dzeHPV (16/18/6/11/44 Kunyora)
Zita rechigadzirwa
HWTS-CC015 Mhando 17 dzeHuman Papillomavirus (16/18/6/11/44 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Gomarara remumuromo wechibereko ndeimwe yemabundu anonyanya kuipa munhengo dzekubereka dzevakadzi. Zvakaratidzwa kuti hutachiona hweHPV hunoramba huripo pamwe nehutachiona hwakawanda ndizvo zvimwe zvezvikonzero zvikuru zvegomarara remumuromo wechibereko. Parizvino kuchine kushaikwa kwemishonga inoshanda inogamuchirwa yegomarara remumuromo wechibereko inokonzerwa neHPV. Saka, kuonekwa nekukurumidza uye kudzivirira hutachiona hwemumuromo wechibereko hunokonzerwa neHPV ndizvo zvakakosha pakudzivirira kenza yemumuromo wechibereko. Kugadzwa kwemiedzo iri nyore, chaiyo uye nekukurumidza yekuongorora hutachiona kwakakosha zvikuru pakuongororwa kwegomarara remumuromo wechibereko.
Chiteshi
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Kudzora kwemukati |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Kudzora kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | -18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Muenzaniso weti, muenzaniso wetsvina yemuromo wemukadzi, muenzaniso wetsvina yemukadzi yekubereka |
| Ct | ≤28 |
| LoD | Makopi 300/mL |
| Kunyatsojeka | Hapana mushonga wekubatanidza utachiona neUreaplasma urealyticum, Chlamydia trachomatis yenzira yekubereka, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella nedzimwe mhando dzeHPV dzisina kuvharwa nekit. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Masisitimu ePCR e480 Enguva Yechokwadi Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinorayirwa chekuburitsa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Wedzera 200μL yemunyu wakajairika kuti udzorere pellet padanho re2.1, uye wobva wabvisa zvichienderana nemirayiridzo yekushandisa reagent iyi yekuburitsa. Vhoriyamu yekubvisa inokurudzirwa i80μL.
Chinorayirwa chekuburitsa mvura chinokurudzirwa: QIAamp DNA Mini Kit (51304) kana Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Wedzera 200μL yemunyu wakajairika kuti udzorerezve pellet iri padanho re2.1, wobva wabvisa mvura yacho zvichienderana nemirayiridzo yekushandisa chinobvumirwa ichi chekuburitsa mvura. Vhoriyamu yesamples yakabviswa yese i200μL, uye vhoriyamu yekudzikisa mvura i100μL.
Reagent inokurudzirwa yekuburitsa: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Wedzera 200μL yereagent yekuburitsa sample kuti udzorere pellet padanho 2.1, uye kubvisa kunofanira kuitwa zvichienderana nemirayiridzo yekushandisa reagent iyi yekuburitsa.
